Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production DOI Creative Commons
Ying Han, Lin Zhang, Deming Rao

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 29, 2025

Chymosin (EC3.4.23.4), primarily sourced from calf abomasum, serves as a conventional coagulant in milk curdling during cheese production. To improve the enzymatic properties and enhance expression of chymosin (BtChy) Kluyveromyces lactis to meet demands industry, silico engineering via hotspot scanning molecular dynamics analysis was adopted. This approach improved activity BtChy on increased its sensitivity at 65°C. Multiple strategies were utilized develop an environmentally friendly method for These included screening constitutive promoters signal peptides, well vitro construction concatemer gene. The optimal combination, comprising P TDH3 promoter, invertase peptide, four-copy gene integrated into yeast genome, identified. After high-density cultivation 5-L bioreactor, recombinant achieved 42,000 SU/mL, 52.5-fold increase over original wild-type

Язык: Английский

Physics-based modeling in the new era of enzyme engineering DOI
Christopher Jurich, Qianzhen Shao, Xinchun Ran

и другие.

Nature Computational Science, Год журнала: 2025, Номер 5(4), С. 279 - 291

Опубликована: Апрель 24, 2025

Язык: Английский

Процитировано

1
Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production DOI Creative Commons
Ying Han, Lin Zhang, Deming Rao

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 29, 2025

Chymosin (EC3.4.23.4), primarily sourced from calf abomasum, serves as a conventional coagulant in milk curdling during cheese production. To improve the enzymatic properties and enhance expression of chymosin (BtChy) Kluyveromyces lactis to meet demands industry, silico engineering via hotspot scanning molecular dynamics analysis was adopted. This approach improved activity BtChy on increased its sensitivity at 65°C. Multiple strategies were utilized develop an environmentally friendly method for These included screening constitutive promoters signal peptides, well vitro construction concatemer gene. The optimal combination, comprising P TDH3 promoter, invertase peptide, four-copy gene integrated into yeast genome, identified. After high-density cultivation 5-L bioreactor, recombinant achieved 42,000 SU/mL, 52.5-fold increase over original wild-type

Язык: Английский

Процитировано

0