Developing a Quantitative Profiling Method for Detecting Free Fatty Acids in Crude Lanolin Based on Analytical Quality by Design DOI Creative Commons
Sihan Liu,

Shaohua Wu,

Hao Zhang

и другие.

Chemosensors, Год журнала: 2025, Номер 13(4), С. 126 - 126

Опубликована: Апрель 3, 2025

In this study, a quantitative profiling method for detecting free fatty acids in crude lanolin based on the Quality by Design (QbD) concept was developed. High-performance liquid chromatography (HPLC) equipped with charged aerosol detector (CAD) and Proshell 120 EC C18 column employed separation of components. Initially, analytical target profile critical attributes were defined. Potential parameters, including temperature, flow rate, isocratic run time, gradient end organic phase ratio, identified using fishbone diagrams single-factor experiments. The definitive screening design (DSD) then utilized to screen optimize these parameters. Stepwise regression applied establish models between Subsequently, operable region (MODR) calculated successfully verified. conditions established configured 0.1% formic acid water acetonitrile serving as mobile phases. rate set at 0.8 mL/min, temperature maintained 35 °C evaporation tube also °C. An injection volume 10 μL used each analysis. elution follows: from 0 30 min, 75% solvent B used, 60 proportion increased 79%. Ten components, 12-hydroxystearic acid, 2-hexyldecanoic palmitic mass spectrometry, seven common peaks found fingerprints. contents oleic stearic quantitatively determined. Both fingerprint analysis methods validated. analyze 15 batches different sources. new can be potentially industrial applications enhance quality control lanolin.

Язык: Английский

Developing a Quantitative Profiling Method for Detecting Free Fatty Acids in Crude Lanolin Based on Analytical Quality by Design DOI Creative Commons
Sihan Liu,

Shaohua Wu,

Hao Zhang

и другие.

Chemosensors, Год журнала: 2025, Номер 13(4), С. 126 - 126

Опубликована: Апрель 3, 2025

In this study, a quantitative profiling method for detecting free fatty acids in crude lanolin based on the Quality by Design (QbD) concept was developed. High-performance liquid chromatography (HPLC) equipped with charged aerosol detector (CAD) and Proshell 120 EC C18 column employed separation of components. Initially, analytical target profile critical attributes were defined. Potential parameters, including temperature, flow rate, isocratic run time, gradient end organic phase ratio, identified using fishbone diagrams single-factor experiments. The definitive screening design (DSD) then utilized to screen optimize these parameters. Stepwise regression applied establish models between Subsequently, operable region (MODR) calculated successfully verified. conditions established configured 0.1% formic acid water acetonitrile serving as mobile phases. rate set at 0.8 mL/min, temperature maintained 35 °C evaporation tube also °C. An injection volume 10 μL used each analysis. elution follows: from 0 30 min, 75% solvent B used, 60 proportion increased 79%. Ten components, 12-hydroxystearic acid, 2-hexyldecanoic palmitic mass spectrometry, seven common peaks found fingerprints. contents oleic stearic quantitatively determined. Both fingerprint analysis methods validated. analyze 15 batches different sources. new can be potentially industrial applications enhance quality control lanolin.

Язык: Английский

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