bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 26, 2024
Abstract
This
study
investigated
the
development
and
characterization
of
decellularized
extracellular
matrix
(dECM)
hydrogels
tailored
for
bio-fabrication
female
reproductive
tissues,
specifically
targeting
cortex,
endometrium,
medulla,
oviduct
tissues.
We
aimed
to
evaluate
cytocompatibility,
biomechanical
properties,
overall
efficacy
these
dECMs
in
promoting
cell
viability,
proliferation,
differentiation.
Our
findings
revealed
that
exhibited
high
biocompatibility
with
embryo
supporting
micro
vascularization
cellular
differentiation
without
need
external
growth
factors.
These
displayed
properties
closely
mimicked
native
which
was
vital
maintaining
their
functional
integrity
activities.
The
printability
assessments
showed
dECMs,
particularly
those
from
cortex
achieved
precision
replicating
intended
structures,
though
challenges
such
as
low
porosity
remained.
bioprinted
constructs
demonstrated
robust
growth,
over
97%
viability
observed
by
day
7,
indicating
suitability
culture.
work
represented
a
significant
advancement
tissue
bio-fabrication,
demonstrating
potential
dECM-based
creating
structurally
functionally
viable
constructs.
By
tailoring
each
dECM
match
unique
different
we
paved
way
more
effective
reliable
applications
medicine
engineering.
Highlights
Developed
bio-inks
Demonstrated
viability.
Achieved
accurate
bioprinting,
structural
integrity.
Promoted
added
Acta Biomaterialia,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 1, 2025
This
study
investigated
the
development
and
characterization
of
decellularized
extracellular
matrix
(dECM)
hydrogels
tailored
for
biofabrication
female
reproductive
tissues,
specifically
targeting
ovarian
cortex,
endometrium,
medulla,
oviduct
tissues.
We
aimed
to
evaluate
cytocompatibility,
biomechanical
properties,
overall
efficacy
these
dECMs
in
promoting
cell
viability,
proliferation,
morphology
using
bovine
model.
Bovine
species
provide
a
valuable
model
due
their
accessibility
from
slaughterhouse
offering
practical
alternative
human
samples,
which
are
often
limited
availability.
Additionally,
tissue
closely
mirrors
certain
physiological
biological
characteristics
humans,
making
it
relevant
translational
research.
Our
findings
revealed
that
exhibited
high
biocompatibility
with
embryo
supporting
micro
vascularization
cellular
without
need
external
growth
factors.
It
is
important
note
addition
alginate
was
crucial
maintaining
structural
integrity
hydrogel
during
long-term
cultures.
These
displayed
properties
mimicked
native
vital
functional
activities.
The
printability
assessments
showed
dECMs,
particularly
those
cortex
achieved
precision
replicating
intended
structures,
though
challenges
such
as
low
porosity
remained.
bioprinted
constructs
demonstrated
robust
growth,
over
97%
viability
observed
by
day
7,
indicating
suitability
culture.
work
represented
significant
advancement
biofabrication,
demonstrating
potential
dECM-based
creating
structurally
viable
constructs.
By
tailoring
each
dECM
match
unique
different
we
paved
way
more
effective
reliable
applications
medicine
engineering.
STATEMENT
OF
SIGNIFICANCE:
research
explores
use
bio-inks
Ovarian
endometrium
essential
processes.
Notably,
microvascularization
differentiation
supplemental
successful
bioprinting
underscores
complex
models.
represents
engineering,
promising
new
avenues
medicine.
Microbiology Research,
Год журнала:
2025,
Номер
16(3), С. 68 - 68
Опубликована: Март 17, 2025
Tilapia
Parvovirus
(TiPV)
is
a
rising
pathogen
responsible
for
high
mortality
in
tilapia
aquaculture.
Understanding
TiPV’s
pathogenesis
crucial
developing
effective
management
strategies.
This
study
aimed
to
elucidate
TiPV
by
evaluating
its
cytotoxic
effects
on
Danio
rerio
gill
(DRG)
cell
monolayers
and
impact
host
immune
responses.
PCR-confirmed
TiPV-infected
DRG
were
subjected
an
MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide)
assay
at
24,
48,
72,
96
h
post-infection
assess
viability
cytotoxicity.
The
revealed
progressive
decline
over
time,
with
viable
percentages
decreasing
from
66.71%
24
31.28%
cultures,
compared
consistently
controls.
Simultaneously,
quantitative
real-time
PCR
(qPCR)
was
used
the
expression
of
key
immune-related
genes,
including
Interleukins
(IL-1β,
IL-8),
Toll-like
receptor
7
(TLR7),
Major
Histocompatibility
Complex
II
(MHC-II),
Tumor
Necrosis
Factor
α
(TNF-α),
Nuclear
Kappa
B
(NF-κB),
Chemokine
Receptors
(CRs).qPCR
analysis
showed
upregulation
IL-8,
IL-1β,
TNF-α,
CRs,
indicating
early
inflammatory
response.
However,
significant
downregulation
TLR7,
MHC-II,
NF-κB
suggests
ability
modulate
results
highlight
that
induces
cytotoxicity
cells,
leading
severe
cellular
damage.
virus
also
alters
responses
modulating
which
may
contribute
virulence
persistence.
These
findings
enhance
our
understanding
need
targeted
research
develop
control
strategies
aquaculture
systems.
Journal of Hazardous Materials,
Год журнала:
2023,
Номер
462, С. 132763 - 132763
Опубликована: Окт. 12, 2023
Multi-walled
carbon
nanotubes
(MWCNTs),
widely
used
in
several
industrial
fields,
are
not
readily
degradable
thus,
persist
environmental
matrices,
serving
as
a
source
of
toxicity
to
organisms.
However,
the
effects
weathering
on
nanomaterial
remain
unclear.
Herein,
we
prepared
aged-MWCNTs
(a-CNTs)
by
incubating
commercial
pristine-MWCNTs
(p-CNTs)
for
two
years
and
compared
their
changes
physicochemical
properties
toxic
zebrafish.
The
characterization
a-CNTs
transmission
electron
microscopy,
X-ray
photoelectron
spectra,
Raman
spectroscopy,
Fourier-transform
infrared
spectroscopy
showed
an
increased
surface
area,
pore
size,
structural
defects,
oxidation
than
those
p-CNTs.
Zebrafish
were
exposed
100
mg/L
p-CNT
a-CNT
four
days.
Subsequently,
mRNA
expression
antioxidant
enzymes,
including
cat,
gst,
sod,
group
1.5-
1.7-fold,
consistent
with
genes
associated
inflammation
(interleukin-8)
apoptosis
(p53)
control.
higher
zebrafish
might
be
due
oxidative
potential
altered
properties.
These
findings
provide
new
insights
into
risk
assessment
management
MWCNTs
aquatic
environment.
further
testing
at
environmentally
relevant
doses,
different
exposure
durations,
diverse
parameters
is
warranted.
Journal of Fish Diseases,
Год журнала:
2024,
Номер
47(5)
Опубликована: Янв. 29, 2024
Abstract
A
permanent
cell
line,
SPB
(Snubnose
pompano
brain)
was
established
from
Trachinotus
blochii
by
the
explant
culture
method.
It
has
been
sub‐cultured
more
than
75
passages
and
showed
optimal
growth
at
28°C
using
L‐15
medium
supplemented
with
15%
to
20%
FBS.
The
cells
were
cryopreserved
different
passage
levels
for
various
applications.
composed
of
fibroblastic
epithelial‐like
cells.
tested
mycoplasma
contamination
which
found
be
negative.
origin
line
T.
confirmed
amplification
mitochondrial
cytochrome
oxidase
I
(COI)
gene.
transfection
efficiency
is
assessed
expression
green
fluorescent
protein
pEGFP‐N1
plasmid.
In
addition,
two
CMV
promotor
plasmids
pFNCPE42‐DNA
pcDNAVP28
transfected
it
shows
high
FNCP
fish
nodavirus
VP28
white
spot
syndrome
virus
immunostaining.
susceptibility
SJNNV
infection
RT‐PCR,
Western
blot,
ELISA,
TCID
50
RT‐qPCR.
Experimental
carried
out
in
propagated
100%
mortality
clinical
signs.
RT‐PCR.
can
used
propagation
viral
pathogens
production
recombinant
proteins.
