Polystyrene microplastic-induced oxidative stress triggers intestinal barrier dysfunction via the NF-κB/NLRP3/IL-1β/MCLK pathway

Environmental Pollution, Год журнала: 2024, Номер 345, С. 123473 - 123473

Опубликована: Янв. 30, 2024

Язык: Английский

---

The release of polylactic acid nanoplastics (PLA-NPLs) from commercial teabags. Obtention, characterization, and hazard effects of true-to-life PLA-NPLs

Journal of Hazardous Materials, Год журнала: 2023, Номер 458, С. 131899 - 131899

Опубликована: Июнь 20, 2023

This study investigates MNPLs release from commercially available teabags and their effects on both undifferentiated monocultures of Caco-2 HT29 in the vitro model intestinal Caco-2/HT29 barrier. Teabags were subjected to mechanical thermodynamic forces simulating preparation a cup tea. The obtained dispersions characterized using TEM, SEM, DLS, LDV, NTA, FTIR. Results confirmed that particles nano-range, constituted by polylactic acid (PLA-NPLs), about one million PLA-NPLs per teabag quantified. internalization, cytotoxicity, intracellular reactive oxygen species induction, as well structural functional changes barrier assessed. show present high uptake rates, especially mucus-secretor cells, bio-persisted tissue after 72 h exposure. Although no significant cytotoxicity was observed exposure 100 µg/mL during 48 h, slight disruption could be detected at short-time periods. work reveals new insights into safety polymer-based teabags, behavior true-to-life human body, questions how repeated prolonged exposures affect structure function epithelium.

Язык: Английский

---

Journey of micronanoplastics with blood components

RSC Advances, Год журнала: 2023, Номер 13(45), С. 31435 - 31459

Опубликована: Янв. 1, 2023

Micronanoplastics (MNPs) interact with blood components, resulting in anemia, cardiovascular diseases, etc. Research gaps include toxic impacts of real-world MNPs, monomers, co-pollutants complex, and so on, emphasizing the need for more research.

Язык: Английский

---

Microplastic pollution in high-altitude Nainital lake, Uttarakhand, India

Environmental Pollution, Год журнала: 2024, Номер 346, С. 123598 - 123598

Опубликована: Фев. 16, 2024

Язык: Английский

---

Effect of micro- and nanoplastic particles on human macrophages

Journal of Hazardous Materials, Год журнала: 2024, Номер 471, С. 134253 - 134253

Опубликована: Апрель 11, 2024

Micro- and nanoplastics (MNPs) are ubiquitous in the environment, resulting uptake of MNPs by a variety organisms, including humans, leading to particle-cell interaction. Human macrophages derived from THP-1 cell lines take up Polystyrene (PS), widespread plastic. The question therefore arises whether primary human also PS micro- nanobeads (MNBs) how they react this stimulation. Major aim study is visualize validate isolation peripheral blood mononuclear cells (PBMCs) assess impact on macrophages. Uptake PBMCs was examined transmission electron microscopy (TEM), scanning live imaging. In addition, reaction analyzed terms metabolic activity, cytotoxicity, production reactive oxygen species (ROS) macrophage polarization. This first MNBs using TEM Metabolic activity size- concentration-dependent, necrosis ROS were increased. methods demonstrated outline an approach influence MNP exposure help investigating consequences worldwide plastic pollution. among particles which can be found different ecosystems (1). Because their size, enter food chain accumulate large organisms (2). Previous studies already showed cytotoxic effect murine dTHP-1 (3, 4). However more research needed distribution humans. Studying potential health risks model, supports process political decision-making addressing

Язык: Английский

---

Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

Environmental Science & Technology, Год журнала: 2024, Номер 58(6), С. 2652 - 2661

Опубликована: Янв. 31, 2024

Micro(nano)plastics (MNPs) are emerging pollutants that can adsorb in the environment and biological molecules ultimately affect human health. However, aspects of adsorption intracellular proteins onto MNPs its effects cells have not been investigated to date. The present study revealed 100 nm polystyrene nanoplastics (NPs) could be internalized by THP-1 specifically adsorbed proteins. In total, 773 NPs with high reliability were identified using proteomics approach analyzed via bioinformatics predict route distribution following cellular internalization. representative Kyoto Encyclopedia Genes Genomes pathway analysis further characterize protein effects. glycolysis through pyruvate kinase M (PKM) adsorption, trigger unfolded response ribophorin 1 (RPN1) heat shock 70 8 (HSPA8), chiefly into clathrin-mediated endocytosis concomitant clathrin heavy chain (CLTC) adsorption. Therefore, this work provides new insights research strategies for caused NPs.

Язык: Английский

---

Genotoxic and neurotoxic potential of intracellular nanoplastics: A review

Journal of Applied Toxicology, Год журнала: 2024, Номер 44(11), С. 1657 - 1678

Опубликована: Март 17, 2024

Abstract Plastic waste comprises polymers of different chemicals that disintegrate into nanoplastic particles (NPLs) 1–100‐nm size, thereby littering the environment and posing a threat to wildlife human health. Research on NPL contamination has up now focused ecotoxicology effects pollution rather than health risks. This review aimed speculate about possible properties carcinogenic neurotoxic as pollutants. Given their low‐dimensional size high surface ratio, NPLs can easily penetrate biological membranes cause functional structural damage in cells. Once inside cell, interrupt autophagy flux cellular debris, alter proteostasis, provoke mitochondrial dysfunctions, induce endoplasmic reticulum stress. Harmful metabolic processes induced by include oxidative stress (OS), ROS generation, pro‐inflammatory reactions. Depending cell cycle status, may direct DNA damage, tumorigenesis, lately carcinogenesis tissues with self‐renewal capabilities like epithelia. In cells able live longest neurons, could trigger neurodegeneration promoting toxic proteinaceous aggregates, OS, chronic inflammation. genotoxicity neurotoxicity are discussed based gathered evidence, when available, within context intracellular uptake these newcomer nanoparticles. summary, this explains how risk evaluation for benefit from accurately monitoring toxicokinetics toxicodynamics at resolution level.

Язык: Английский

---

Photoaging Promotes Toxic Micro/Nanoplastics Release from PLA/PBAT Biodegradable Plastic in Gastrointestinal Condition

Environment & Health, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

The release of micro/nanoplastics (MNPs) from biodegradable plastics in gastrointestinal environments due to photoaging, along with their associated mechanisms and potential cytotoxicity, is largely unknown. Here, we show that poly(lactic acid)/poly(butylene adipate-co-terephthalate) (PLA/PBAT) films undergo ultraviolet resulting increased surface roughness a higher quantity MNPs on the surface. This aging process involves generation carbon- oxygen-centered free radicals, chain scission, formation oxidation products hydroxyl carbonyl groups. These can be released under water shear force, significantly increasing normalized mass loss aged approximately 0.128 mg/cm2 (18 times than unaged water). In environment, further increases about 0.196 (28 higher), likely enzymatic digestion ion-swelling effects. MNPs, primarily composed PLA, are smaller carry more negative charges conditions. THP-1 cell model, these affect viability dose-dependent manner. obtained through ultrafiltration, compared those collected via centrifugation, display broader size distribution induce pronounced toxicity cells, an EC50 243 mg/L. Preliminary comparative analysis indicates PLA/PBAT-derived present risks comparable to, or greater than, conventional plastic MNPs. findings underscore hazards plastics.

Язык: Английский

---

Potential lifetime effects caused by cellular uptake of nanoplastics: A review

Environmental Pollution, Год журнала: 2023, Номер 329, С. 121668 - 121668

Опубликована: Апрель 20, 2023

Язык: Английский

---

Harmful effects of true-to-life nanoplastics derived from PET water bottles in human alveolar macrophages.

Environmental Pollution, Год журнала: 2024, Номер 348, С. 123823 - 123823

Опубликована: Март 19, 2024

The increasing presence of secondary micro/nanoplastics (MNPLs) in the environment requires knowing if they represent a real health concern. To such end, an important point is to test representative MNPLs as denominated true-to-life MNPLs, resulting from degradation plastic goods lab conditions. In this study, we have used polyethylene terephthalate (PET) NPLs PET water bottles. Since inhalation exposure route environmental MNPLS, mouse alveolar macrophages (MH-S) target cell, and study focused only on cells that internalized them. This type approach novel it may capture realistic adverse effects PETNPLs cells, thereby mitigating any biases while assessing risk these MNPLs. Furthermore, utilized set biomarkers including intracellular reactive oxygen species (ROS) levels, variations mitochondrial membrane potential values, macrophage polarization M1 (pro-inflammatory response) M2 (anti-proinflammatory possible cellular due PETNPLs. After exposures lasting for 3 24 h range concentrations (0, 25, 50, 100 μg/mL) results indicate no toxicity was induced despite 100% internalization observed at highest concentration. Significant levels ROS were observed, mainly h, indirect concentration-effect relationship. Interestingly, reduction but without clear Finally, PETNPL shows significant M0 subtypes. Polarization stage) more marked occurred both times. (anti-inflammatory after h. Due relevance described biomarkers, our underscore need further research, better understand implications associated with MNPL exposure.

Язык: Английский

---