Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 23, 2024
Identifying
the
signatures
of
intestinal
dysbiosis
caused
by
common
stresses
is
fundamental
to
establishing
efficient
health
monitoring
strategies
for
sea
cucumber.
This
study
investigated
impact
six
stress
experienced
frequently
in
aquaculture
on
growth
performance,
homeostasis
and
microbiota
cucumber,
including
thermal
(23°C),
hypoosmotic
(22‰
salinity),
ammonium
(0.5
mg/L
NH
4
+
-N),
nitrite
(0.25
NO
2
−
-N)
exposure
30
days,
as
well
starvation
crowding
(6
kg/m
3
density)
60
days.
Results
demonstrated
that
all
led
reduced
performance
digestive
capacity
along
with
varying
degrees
oxidative
immune
responses.
Various
significantly
altered
diversity,
community
structure
(except
stress),
composition
microbiota.
The
ratios
Bacteroidota:
Proteobacteria
(B:
P)
Firmicutes:
(F:
declined
markedly
compared
control.
Potentially
pathogenic
bacteria
Shewanellaceae,
Vibrionaceae,
Moraxellaceae
increased
under
crowding,
ammonium,
stress,
respectively,
whereas
beneficial
microbes
Achromobacter
Rhodobacteraceae
were,
enriched
stresses.
complexity
stability
microbial
ecological
networks
were
further
these
KEGG
predictions
revealed
functional
pathways
involved
host
immunity
different
Correlation
analysis
confirmed
a
strong
link
between
response
abundance
Verrucomicrobia
species
could
also
be
identified
sensitive
indicator
diagnosing
whether
was
stressful
pressure
random
forest
analysis.
Ecotoxicology and Environmental Safety,
Год журнала:
2023,
Номер
262, С. 115174 - 115174
Опубликована: Июнь 22, 2023
Owing
to
their
potential
adverse
health
effects,
global
contamination
by
microplastics
(MPs)
has
attracted
increased
scientific
and
societal
concerns.
However,
in
vivo
studies
on
MP
toxicity,
along
with
its
effects
underlying
mechanisms,
remain
limited.
We
recently
found
that
non-coding
RNA
(ncRNAs)
contribute
MP-mediated
vascular
toxicity.
Moreover,
previous
have
identified
N6-methyladenosine
(m6A)
modifications
ncRNAs
as
influencing
factors
cardiovascular
disease.
whether
how
m6A
are
affected
MP-induced
cardiotoxicity
unknown.
Herein,
we
profiled
differentially
expressed
related
modification
profiles
MP-exposed
myocardial
tissue
using
sequencing
(RNA-seq)
methylated
immunoprecipitation
(MeRIP-seq).
First,
observed
MPs
accumulated
different
organs
upregulated
apoptosis
the
heart,
liver,
spleen,
kidney
cells.
Furthermore,
total
METTL3
levels
myocardium
after
exposure
MPs.
RNA-seq
results
revealed
392
lncRNAs
302
circRNAs
were
MP-treated
mouse
compared
control
group.
Gene
Ontology
Kyoto
Encyclopedia
of
Genes
Genomes
enrichment
analyses
showed
these
altered
closely
associated
endocytosis,
cellular
senescence,
cell
cycle
signaling
pathways,
which
may
cause
cardiotoxicity.
MeRIP-seq
data
distributions
abundances
circRNAs.
Additionally,
through
conjoint
analysis
two
high-throughput
datasets
both
expression
circ-Arfgef2
lncG3bp2
This
suggests
involved
Our
findings
a
better
understanding
new
molecular
targets
for
treating
cardiac
injury.
