Transcriptomics pave the way into mechanisms of cobalt and nickel toxicity: Nrf2-mediated cellular responses in liver carcinoma cells DOI Creative Commons
Alicia Thiel, Franziska Drews, Marcello Pirritano

и другие.

Redox Biology, Год журнала: 2024, Номер 75, С. 103290 - 103290

Опубликована: Июль 27, 2024

Cobalt (Co) and Nickel (Ni) are used nowadays in various industrial applications like lithium-ion batteries, raising concerns about their environmental release public health threats. Both metals potentially carcinogenic may cause neurological cardiovascular dysfunctions, though underlying toxicity mechanisms have to be further elucidated. This study employs untargeted transcriptomics analyze downstream cellular effects of individual combined Co Ni human liver carcinoma cells (HepG2). The results reveal a synergistic effect Ni, leading significantly higher number differentially expressed genes (DEGs) compared exposure. There was clear enrichment Nrf2 regulated linked pathways such as glycolysis, iron glutathione metabolism, sphingolipid confirmed by targeted analysis. exposure alone caused nuclear translocation, while only affects evidenced upregulation HMOX-1 storage protein FTL. impact increasing dihydroceramide levels decreasing ceramides, sphingosine lactosylceramides, along with diacylglycerol accumulation. By combining analytical methods, this provides valuable insights into molecular toxicity, paving the way for understanding metal stress.

Язык: Английский

The adsorption behavior and mechanism of Cu(Ⅱ), Fe(Ⅱ) and Co(Ⅱ) on straw biochar and their Fenton-like performance for ciprofloxacin decontamination DOI
Qinyu Yang, Jie Gao, Ting Guo

и другие.

Journal of Environmental Management, Год журнала: 2025, Номер 373, С. 123962 - 123962

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Genotoxicity Assessment of Co(II) and Ni(II) in HepG2 Cells: Insights into Combined Metal Exposure DOI
Alicia Thiel,

Sarah Heider,

Kira Bieck

и другие.

Chemical Research in Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

The usage of cobalt (Co) and nickel (Ni) in numerous commercial, industrial, military applications causes widespread exposure nowadays, concerns are rising about adverse impacts on human health. Emphasis is the respiratory system, with both metals classified as (possibly) carcinogenic upon inhalation by International Agency for Research Cancer (IARC), but limited data available oral exposure. Therefore, this study aims to evaluate vitro genotoxicity Co(II) Ni(II) their combination HepG2 cells, since those environmental pollutants occurs realistically concert. Here, led induction single-strand breaks oxidative DNA damage detected Comet assay FPG-sensitive sites, while increased abundance γ-H2AX, an indicator double-strand breaks. Notably, combined resulted enhanced damage, especially at chromosomal level, formation micronuclei well polynucleated indicating a stronger effect compared single Furthermore, induced response pathway PARylation. As process involves consumption large amounts cellular NAD+ after energy state was assessed Ni(II). Current indicate that altered state. This reveals distinct mechanisms exhibited Ni(II), which were treatment. highlights need further research estimate genotoxic potential targeting cells intake increasing entry.

Язык: Английский

Процитировано

0

Fractionation, spatial distribution, ecological and health risk assessment of cobalt and nickel in surface sediment of a bay along the southeast coast of China DOI
Yang Liu,

Weiming Kuang,

Xiuwu Sun

и другие.

Marine Pollution Bulletin, Год журнала: 2024, Номер 206, С. 116786 - 116786

Опубликована: Авг. 1, 2024

Язык: Английский

Процитировано

3

Transcriptomics pave the way into mechanisms of cobalt and nickel toxicity: Nrf2-mediated cellular responses in liver carcinoma cells DOI Creative Commons
Alicia Thiel, Franziska Drews, Marcello Pirritano

и другие.

Redox Biology, Год журнала: 2024, Номер 75, С. 103290 - 103290

Опубликована: Июль 27, 2024

Cobalt (Co) and Nickel (Ni) are used nowadays in various industrial applications like lithium-ion batteries, raising concerns about their environmental release public health threats. Both metals potentially carcinogenic may cause neurological cardiovascular dysfunctions, though underlying toxicity mechanisms have to be further elucidated. This study employs untargeted transcriptomics analyze downstream cellular effects of individual combined Co Ni human liver carcinoma cells (HepG2). The results reveal a synergistic effect Ni, leading significantly higher number differentially expressed genes (DEGs) compared exposure. There was clear enrichment Nrf2 regulated linked pathways such as glycolysis, iron glutathione metabolism, sphingolipid confirmed by targeted analysis. exposure alone caused nuclear translocation, while only affects evidenced upregulation HMOX-1 storage protein FTL. impact increasing dihydroceramide levels decreasing ceramides, sphingosine lactosylceramides, along with diacylglycerol accumulation. By combining analytical methods, this provides valuable insights into molecular toxicity, paving the way for understanding metal stress.

Язык: Английский

Процитировано

2