Integrative biomonitoring in Litopenaeus vannamei: Metal analysis and biochemical markers

Marine Pollution Bulletin, Год журнала: 2025, Номер 212, С. 117544 - 117544

Опубликована: Янв. 14, 2025

Язык: Английский

Emerging Resistance and Virulence Patterns in Salmonella enterica: Insights into Silver Nanoparticles as an Antimicrobial Strategy

Antibiotics, Год журнала: 2025, Номер 14(1), С. 46 - 46

Опубликована: Янв. 7, 2025

Background/Objectives: This study aims to characterize antibiotic resistance (AR) and virulence markers in Salmonella spp. isolated from Romanian outpatients’ stool samples. Methods: In 2019, community-acquired strains were collected identified using MALDI-TOF mass spectrometry, susceptibility profiles have been determined with the MicroScan system, soluble factors evaluated specific culture media, while biofilm formation was quantified 96-well plates. Molecular analysis targeted genes for β-lactams (e.g., blaTEM blaSHV); tetracyclines tet(A)); sulphonamides; quinolones, as well invA, spvC, pldA, held). Whole-genome sequencing (WGS) performed on 19 selected isolates. A silver nanoparticles (AgNPsol) alternative conventional antibiotics tested effectiveness against multidrug-resistant (MDR) Results: From total of 309 isolates (65.05% children under 4 years age) belonging four subtypes serovars, 27.86% showed at least one antibiotic, most frequently tetracycline, ampicillin, piperacillin. The expressed haemolysin (67%), aesculinase (65%), gelatinase (62%). Resistance trimethoprim-sulfamethoxazole encoded by sul1 gene 44.83% tet(A) (59.52%). ESBL blaTEM, blaSHV, blaCTX-M detected PCR 16.18%, 2.91%, 0.65% strains, respectively. Additionally, 98.63% carried invA marker, notable positive associations between qnrB, spvC. Conclusions: present findings revealed significant patterns isolates, subtypes, AR, virulence, emphasising need continuous surveillance infections. potential AgNPs an treatment option demonstrated, particularly paediatric S. enterica

Язык: Английский

Effects of environmental factors on the oxidative status of Anemonia viridis in aquaculture systems

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, Год журнала: 2024, Номер unknown, С. 111042 - 111042

Опубликована: Окт. 1, 2024

Язык: Английский