bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 12, 2024
Abstract
The
race-specific
resistance
gene
Lr14a
in
wheat
confers
a
unique
type
of
heterogenous
reaction.
It
encodes
an
ankyrin-repeat
transmembrane
domain
protein
that
immunity
against
the
fungal
pathogen
Puccinia
triticina
.
Here,
we
show
functions
as
calcium-permeable
channel,
mediating
cytoplasmic
Ca²⁺
influx
is
crucial
for
leaf
rust
wheat.
Infection
with
avirulent
isolates
induced
expression
predominantly
mesophyll
cells
while
triggering
cell
death
guard
This
study
revealed
mechanism
by
which
product
R
regulates
stomatal
non-cell
autonomously
through
mediation
calcium
signaling.
Nature,
Год журнала:
2024,
Номер
632(8026), С. 869 - 876
Опубликована: Июнь 12, 2024
Abstract
Nucleotide-binding
leucine-rich
repeat
(NLR)
proteins
play
a
pivotal
role
in
plant
immunity
by
recognizing
pathogen
effectors
1,2
.
Maintaining
balanced
immune
response
is
crucial,
as
excessive
NLR
expression
can
lead
to
unintended
autoimmunity
3,4
Unlike
most
NLRs,
the
required
for
cell
death
2
(NRC2)
belongs
small
group
characterized
constitutively
high
without
self-activation
5
The
mechanisms
underlying
NRC2
autoinhibition
and
activation
are
not
yet
understood.
Here
we
show
that
Solanum
lycopersicum
(tomato)
(
Sl
NRC2)
forms
dimers
tetramers
higher-order
oligomers
at
elevated
concentrations.
Cryo-electron
microscopy
shows
an
inactive
conformation
of
these
oligomers.
Dimerization
oligomerization
only
stabilize
state
but
also
sequester
from
assembling
into
active
form.
Mutations
dimeric
or
interdimeric
interfaces
enhance
pathogen-induced
Nicotiana
benthamiana
cryo-electron
structures
unexpectedly
inositol
hexakisphosphate
(IP
6
)
pentakisphosphate
bound
inner
surface
C-terminal
domain
NRC2,
confirmed
mass
spectrometry.
phosphate-binding
site
impair
phosphate
binding
NRC2-mediated
N.
Our
study
indicates
negative
regulatory
mechanism
suggests
phosphates
cofactors
NRCs.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 2, 2024
Many
disease
resistance
genes
have
been
introgressed
into
wheat
from
its
wild
relatives.
However,
reduced
recombination
within
the
segments
hinders
cloning
of
genes.
Here,
we
cloned
powdery
mildew
gene
Pm13,
which
is
Aegilops
longissima,
using
a
method
that
combines
physical
mapping
with
radiation-induced
chromosomal
aberrations
and
transcriptome
sequencing
analysis
ethyl
methanesulfonate
(EMS)-induced
loss-of-function
mutants.
Pm13
encodes
kinase
fusion
protein,
designated
MLKL-K,
an
N-terminal
domain
mixed
lineage
domain-like
protein
(MLKL_NTD
domain)
C-terminal
serine/threonine
bridged
by
brace.
The
function
validated
through
transient
stable
transgenic
complementation
assays.
Transient
over-expression
analyses
in
Nicotiana
benthamiana
leaves
protoplasts
reveal
fragment
Brace-Kinase122-476
MLKL-K
capable
inducing
cell
death,
dependent
on
functional
three
α-helices
brace
region
close
to
N-terminus
domain.
was
longissima.
authors
report
it
MLKL_NTD
domain-containing
kinase.
New Crops,
Год журнала:
2024,
Номер
1, С. 100027 - 100027
Опубликована: Май 18, 2024
Calcium
ions
(Ca2+)
serve
as
key
messengers
in
plant
immune
reactions.
A
typical
Ca2+
signaling
involves
three
steps:
encoding
specific
signatures
by
Ca2+-permeable
channels,
decoding
signals
sensors,
and
downstream
responses.
This
review
focuses
on
plasma
membrane-localized
channels
cytosolic
unraveling
their
roles
influx
during
pattern-triggered
immunity,
effector-triggered
autoimmunity.
Several
unresolved
questions
were
highlighted,
including
the
regulation
of
channel
activity
for
induction
mechanism
behind
influx-triggered
hypersensitive
response
cell
death.
concise
overview
provides
insights
into
complex
interplay
paving
way
future
investigations
molecular
plant-microbe
interactions.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 6, 2025
Abstract
The
race-specific
resistance
gene
Lr14a
in
wheat
confers
a
unique
type
of
heterogenous
reaction.
It
encodes
an
ankyrin-repeat
transmembrane
domain
protein
that
immunity
against
the
fungal
pathogen
Puccinia
triticina.
Here,
we
show
Lr14a
functions
as
calcium-permeable
channel,
mediating
cytoplasmic
Ca²⁺
influx
is
crucial
for
leaf
rust
wheat.
Infection
with
avirulent
isolates
induced
expression
predominantly
mesophyll
cells
while
triggering
cell
death
guard
This
study
revealed
mechanism
by
which
product
R
regulates
stomatal
non-cell
autonomously
through
mediation
calcium
signaling.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 3, 2025
ABSTRACT
Plants
utilize
cell
surface-localized
pattern
recognition
receptors
(PRRs)
and
intracellular
nucleotide-binding
leucine-rich
repeat
(NLR)
to
detect
non-self
elicit
robust
immune
responses.
Fine-tuning
the
homeostasis
of
these
is
critical
prevent
their
hyperactivation.
Here,
we
show
that
Arabidopsis
plants
lacking
metacaspase
1
(
At
MC1)
display
autoimmunity
dependent
on
signalling
components
downstream
NLR
PRR
activation.
Overexpression
a
catalytically
inactive
MC1
in
an
atmc1
background
triggers
severe
partially
same
components.
E3
ligase
SNIPER1,
master
regulator
homeostasis,
fully
reverts
AtMC1-dependent
phenotype,
inferring
broad
defect
turnover
may
underlie
phenotype
observed.
Catalytically
localizes
punctate
structures
are
degraded
through
autophagy.
Altogether
considering
previous
evidence
proteostatic
functions
MC1,
speculate
Wt
either
directly
or
indirectly
control
protein
levels,
thereby
preventing
autoimmunity.
