Guava Leaf Extract Exhibits Antimicrobial Activity in Extensively Drug-Resistant (XDR) Acinetobacter baumannii DOI Creative Commons
Daniela Gutiérrez Montiel, Alma L. Guerrero-Barrera, Flor Yazmín Ramírez Castillo

и другие.

Molecules, Год журнала: 2024, Номер 30(1), С. 70 - 70

Опубликована: Дек. 28, 2024

Currently, a global health crisis is being caused by microbial resistance, in which Acinetobacter baumannii plays crucial role, considered the highest-priority microorganism World Health Organization (WHO) for discovering new antibiotics. As result, phytochemicals have emerged as potential alternative to combat resistant strains, since they can exert antimicrobial activity through various mechanisms and, at same time, represent more natural and safe option. This study analyzes effects of guava leaf extract ten clinical isolates extensively drug-resistant (XDR) A. baumannii, using agar diffusion technique microdilution method determine minimum inhibitory concentrations (MICs). Additionally, possible improvements after purification polyphenolic compounds synergy with antibiotic gentamicin are examined this research. Moreover, effect plant cell line A549 derived from lung tissue was also evaluated. The exhibited against all strains studied, polyphenols along combination improved activity. presence induced morphological changes cells 24 h exposure. Therefore, Psidium guajava L. agent.

Язык: Английский

MM/GB(PB)SA integrated with molecular docking and ADMET approach to inhibit the fat-mass-and-obesity-associated protein using bioactive compounds derived from food plants used in traditional Chinese medicine DOI Creative Commons
Manisha Mandal, Shyamapada Mandal

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2024, Номер 11, С. 100435 - 100435

Опубликована: Май 3, 2024

Obesity is currently a major global concern, requiring the development of effective therapeutics. This study determines inhibitory activity against FTO (fat mass and obesity-associated protein) food plant-derived bioactive compounds TCM (traditional Chinese medicine) importance, by in silico approaches. Food phytochemicals, such as curcumin (CCMN), cajaisoflavone (CJIF), cyanidin-3-O-glucoside (C3OG) pelargonidin-3-glucoside (P3GS), were selected ligands, docked to FTO. The reference FTO-inhibitor used was meclofenmic acid (MCFA). MDS (molecular dynamic simulations) accomplished with 10 ns run for complex most potential phytocompound. phytochemicals subjected bioactivity scoring, Lipinski's rule five screening, ADMET prediction. Law Molecular docking demonstrated binding energies -9.4, -8.8, -8.7 -8.2 kcal/mol CCMN, CJIF, C3OG P3GS, respectively. inhibitor MCFA had energy -7.8 kcal/mol, therefore, inhibition capacity ligands order CCMN > P3GS CJIF MCFA. residues His231, Ser229, Val228, Leu109, Tyr108 Pro93 played crucial role protein-ligands interaction displaying hydrogen bonds hydrophobic contacts. MDS-based MM/GB(PB)SA free authenticated (-6.67/-8.77 kcal/mol) stronger than (0.19/-0.02 kcal/mol). Pharmacological properties predicted comparable standard. botanical origin are important management obesity. In current study, all ingredients, acceptable pharmacological displayed excellent affinity FTO, compared FTO_CCMN more energetic stability FTO_MCFA using Overall, this paved basis FTO-associated obesity plant-based therapeutics importance globe.

Язык: Английский

Процитировано

15

The Antimicrobial Effects of Coffee and By-Products and Their Potential Applications in Healthcare and Agricultural Sectors: A State-of-Art Review DOI Creative Commons

Rosa Castro-Díaz,

Norma Patricia Silva-Beltrán, Nohemí Gámez‐Meza

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 215 - 215

Опубликована: Янв. 21, 2025

Coffee is one of the most consumed beverages around world. Its production dominated by species Coffea arabica and canephora. However, coffee elaboration process leads to generating a significant amount waste, which arises in various stages bean processing rich natural bioactive compounds such as phenolic alkaloids. Particularly, chlorogenic caffeic acids have high antimicrobial potential been demonstrated be effective against bacteria viruses healthcare food relevance, including multi-resistant pathogens. accumulation waste negative environmental impact since they can contaminate surrounding environment due presence organic molecules caffeine tannins. In this context, exploiting resources source with of, for example, obtained from coffee, has evaluated previous works. This review aims summarize current knowledge on properties its by-products their application sector disease control agricultural crops, particular emphasis improving sustainability efficiency agriculture through making use carries importance today’s society.

Язык: Английский

Процитировано

2

MurG as a potential target of quercetin in Staphylococcus aureus supported by evidence from subtractive proteomics and molecular dynamics DOI Creative Commons
Dweipayan Goswami, Jignesh Prajapati, Milan Dabhi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 1, 2025

The rise of methicillin-resistant Staphylococcus aureus (MRSA) as a major public health threat underscores critical need for new antibacterial strategies. Quercetin is naturally occurring flavonoid with range bioactivities, including activity against S. aureus. However, how quercetin inhibits and binds to its potential molecular target not well understood. Understanding the interaction bacterial targets may provide crucial insights developing modified derivatives better drug-like properties. To investigate in aureus, we employed targeted subtractive proteomics approach, which identified glycosyltransferase MurG novel target. Through rigorous docking extensive 250 ns dynamics simulations, was shown bind stably MurG, suggesting mechanism that interferes peptidoglycan biosynthesis pathway. Molecular Mechanics Generalized Born Surface Area (MM-GBSA) analyses provided quantitative evidence complex's stability, indicating strong stable therapeutic implications. Principal Component Analysis (PCA) further validated reduction MurG's structural flexibility upon binding, reinforcing hypothesis this could effectively inhibit biological function. identification provides foundation development novel, more effective agents. This strategy, facilitated by proteomics, also be adapted other resistant pathogens, demonstrating broad applicability fight antibiotic resistance.

Язык: Английский

Процитировано

2

Evaluation of inhibitory efficacy of plantaricin JK against NSP1 from SARS-CoV-2 by in silico methods DOI Creative Commons
Manisha Mandal, Shyamapada Mandal

Aspects of Molecular Medicine, Год журнала: 2025, Номер unknown, С. 100080 - 100080

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1

Antibacterial activity analysis of kaempferol and its derivatives targeting virulence and quorum sensing associated proteins by in silico methods DOI Creative Commons
Golak Majumdar, Shyamapada Mandal

The Microbe, Год журнала: 2025, Номер unknown, С. 100259 - 100259

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1

Quercetin and its nanoformulations as promising agents for lung cancer treatment: A focus on molecular mechanisms DOI
Reza Arefnezhad,

Sara Sepehri Amin,

Azam Mohammdi

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 99, С. 105933 - 105933

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

3

Anti-dengue Viral Protein NS5 and Antibacterial Efficacy of Andrographolide from Andrographis paniculata: An In Silico Approach DOI
Kandasamy Selvam, Chinnappan Sudhakar,

Arunagiri Ragu Prasath

и другие.

Applied Biochemistry and Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Язык: Английский

Процитировано

0

The Impact of Plant-Derived Polyphenols on Combating Efflux-Mediated Antibiotic Resistance DOI Open Access
Anna Duda-Madej, Szymon Viscardi,

Piotr Niezgódka

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4030 - 4030

Опубликована: Апрель 24, 2025

The global healthcare system is increasingly challenged by the rising prevalence of multidrug-resistant bacteria and limited therapeutic options for related infections. Efflux-mediated antibiotic resistance represents a significant obstacle, primarily due to absence drugs specifically designed target bacterial efflux pumps. Recent research has identified polyphenols, broad class plant-derived organic compounds, as potential inhibitors pump activity. This review consolidates data on inhibitory properties eight widely distributed polyphenols: curcumin, quercetin, luteolin, tannic acid, naringenin, epigallocatechin-3-gallate, ellagic resveratrol. These compounds have demonstrated capacity inhibit pumps, either through direct interference with protein function or downregulating expression genes encoding subunits. Importantly, several polyphenols exhibit synergistic interactions antibiotics, including colistin, ciprofloxacin, tetracycline. For instance, quercetin shown potency comparable that established such verapamil reserpine. findings suggest represent promising candidates development novel inhibitors. However, further required validate their efficacy safety facilitate translation into clinical applications combating resistance.

Язык: Английский

Процитировано

0

Antimicrobial Efficacy of Fluoride Varnish with Quercetin against Candida albicans and Enterococcus faecalis: An In Vitro Study DOI Creative Commons

Bhuvaneswari Natarajan,

Ganesh Jeevanandan, Prabhadevi C Maganur

и другие.

European Journal of General Dentistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Abstract Objective Fluoride varnish is a preventive management of dental caries. Matrix metalloproteinases (MMPs) play major role in the degradation extracellular matrix process caries progression. Quercetin, natural flavonoid, has literature background with marked MMP inhibitor activity. Till date, there no fluoride that both remineralizing effect and prevention dentin breakdown at same time. Therefore, novel containing tricalcium phosphate, quercetin, sodium formulated to have greater action while also preventing deterioration dentinal matrix. So, this study aimed evaluate antimicrobial efficacy quercetin against Enterococcus faecalis Candida albicans. Materials Methods The was prepared tested for potential applications. activity different concentrations (25, 50, 100 μL) oral pathogens (E. C. albicans) evaluated using agar well diffusion method, protein leakage assay, cytoplasmic time kill curve assay. Results showed significant inhibition zone albicans E. method. assays dose-dependent relationships increased higher (p < 0.05). Conclusion better than commercial varnish.

Язык: Английский

Процитировано

0

The Antibacterial Activity of Cornus mas L. Against Uropathogenic Escherichia coli Strains Producing Extended-Spectrum β-Lactamase DOI
Gülsen Kendir, Suna Kızılyıldırım, Tülay Kandemir

и другие.

Microbial Pathogenesis, Год журнала: 2025, Номер 205, С. 107677 - 107677

Опубликована: Май 6, 2025

Язык: Английский

Процитировано

0