Establishment and application of novel transient cotyledon and seed transformation systems in Tartary buckwheat
Plant Cell Tissue and Organ Culture (PCTOC),
Год журнала:
2025,
Номер
160(2)
Опубликована: Фев. 1, 2025
Язык: Английский
Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing
Plants,
Год журнала:
2025,
Номер
14(6), С. 889 - 889
Опубликована: Март 12, 2025
Recent
years
have
witnessed
remarkable
progress
in
plant
biology,
driven
largely
by
the
rapid
evolution
of
CRISPR/Cas-based
genome
editing
(GE)
technologies.
These
tools,
including
versatile
CRISPR/Cas
systems
and
their
derivatives,
such
as
base
editors
prime
editors,
significantly
enhanced
universality,
efficiency,
convenience
functional
genomics,
genetics,
molecular
breeding.
However,
traditional
genetic
transformation
methods
are
essential
for
obtaining
GE
plants.
depend
on
tissue
culture
procedures,
which
time-consuming,
labor-intensive,
genotype-dependent,
challenging
to
regenerate.
Here,
we
systematically
outline
current
advancements
simplifying
GE,
focusing
optimization
process
through
developmental
regulators,
development
planta
methods,
establishment
nanomaterial-
viral
vector-based
delivery
platforms.
We
also
discuss
critical
challenges
future
directions
achieving
genotype-independent,
culture-free
GE.
Язык: Английский
Optimization of the method of Agrobacterium-mediated in planta transformation of winter wheat genotypes
Faktori eksperimental noi evolucii organizmiv,
Год журнала:
2024,
Номер
34, С. 206 - 211
Опубликована: Сен. 25, 2024
Aim.
Optimization
of
the
Agrobacterium-mediated
in
planta
transformation
winter
wheat
genotypes.
Methods.
Genetic
transformation,
molecular
genetic
analysis;
mathematical
statistics.
Results.
The
influence
composition
inoculation
medium
and
method
application
agrobacterium
cell
suspension
on
frequency
seed
setting
formation
transgenic
plants
new
promising
genotypes
was
studied.
dependence
obtaining
amount
grain
transformants
genotypic
characteristics
when
using
different
conditions
procedures
established.
higher
efficiency
MC-32
medium,
which
additionally
contained
MES,
magnesium
sulfate,
ammonium
chloride,
shown
compared
to
MC-31
supplemented
with
sodium
thiosulfate.
A
significant
difference
rate
revealed
methods
applying
A.
tumefacins
suspension.
Method
I
proved
be
most
effective,
a
bacterial
culture
applied
pistil
receptacle,
after
liquid
agrobacteria
were
resuspended
completely
dried,
pollination
carried
out
pollen
obtained
from
an
intact
ear
same
plant.
Conclusions.
Selected
for
efficient
gene
transfer
process
Язык: Английский