Stress responsive glycosylphosphatidylinositol-anchored protein SsGSP1 contributes to sclerotinia sclerotiorum virulence DOI Creative Commons
Hongmei Liao, Yangui Chen,

Yujia He

и другие.

Virulence, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Fungal cell wall acts as a defence barrier, shielding the from varying environmental stresses. Cell proteins, such glycosylphosphatidylinositol (GPI)-anchored are involved in swift and appropriate responses to minor changes fungi. However, roles of these proteins pathogenic Sclerotinia sclerotiorum remain largely unexplored. Here, we identified novel GPI-anchored protein S. sclerotiorum, SsGSP1, comprising Kre9_KNH domain. SsGSP1 was upregulated during infection, loss-of-function mutants exhibited compromised integrity reduced β-glucan content. During inoculation on Arabidopsis thaliana, Nicotiana benthamiana, Brassica napus, SsGSP1-deletion strains demonstrated decreased virulence. The transgenic A. thaliana line carrying sRNA targeting enhanced resistance via Host-Induced Gene Silencing (HIGS). SsGSP1-deficient displayed heightened sensitivity various stresses, including osmotic pressure, oxidative stress, heat shock. yeast two-hybrid BiFC assays confirmed that interacted with key stress-related catalase SsCat2, shock Sshsp60, ABC transporter SsBMR1. Accordingly, transcriptome analysis revealed disruption downregulated expression genes stress response, chemical agent resistance. These results collectively delineate intricate role β-glucan, virulence, may act potential surface sensor elicit signal transduction response stresses sclerotiorum.

Язык: Английский

Unveiling the role of microRNAs in nonhost resistance to Sclerotinia sclerotiorum: Rice‐specific microRNAs attack the pathogen via cross‐kingdom RNAi DOI Open Access
Jiaqin Mei,

Shuxian Yang,

Yanxia Linghu

и другие.

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

The development of rapeseed with high resistance against the pathogen Sclerotinia sclerotiorum is impeded by lack effective resources within host species. Unraveling molecular basis nonhost (NHR) holds substantial value for improvement in crops. In present study, small RNA sequencing and transcriptome were carried out between rice (a species S. sclerotiorum) during infection, revealing involvement miRNAs on translation-related processes both pathogen. Specifically, rice-specific potential capability cross-kingdom RNAi explored, which Os-miR169y was selected as a representative case to elucidate its role sclerotiorum. silence decreased level sclerotiorum, heterologous expression Arabidopsis significantly enhanced resistance. dual-luciferase reporter assay indicates that targets 60S ribosomal protein L19 (SsRPL19). Overexpressing (OEss-miR169y) SsRPL19 (RNAiss-RPL19) impaired growth pathogenicity pathogen, while overexpressing exhibited contrast effect. Yeast-two-hybridization revealed an interlinking multiple large subunits, indicating important translation. Proteome detected amount proteins transformants OEss-miR169y RNAiss-RPL19 significant suppression key metabolic pathways such carbon nitrogen metabolisms. Collectively, this study suggests can secrete specific suppress genes essential Os-miR169y, suppresses thus impairs synthesis This sheds light intrinsic mechanisms NHR further demonstrates using nonhost-specific "pathogen-attacking" improving

Язык: Английский

Процитировано

2
Identifying the role of cellulase gene CsCEL20 upon the infection of Xanthomonas citri subsp. citri in citrus DOI
Yi Li, Heqiang Lou,

Hongyan Fu

и другие.

Molecular Breeding, Год журнала: 2025, Номер 45(1)

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
New insights into plant cell wall functions DOI
Lanjun Zhang, Chengxu Gao, Yihong Gao

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Stress responsive glycosylphosphatidylinositol-anchored protein SsGSP1 contributes to sclerotinia sclerotiorum virulence DOI Creative Commons
Hongmei Liao, Yangui Chen,

Yujia He

и другие.

Virulence, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Fungal cell wall acts as a defence barrier, shielding the from varying environmental stresses. Cell proteins, such glycosylphosphatidylinositol (GPI)-anchored are involved in swift and appropriate responses to minor changes fungi. However, roles of these proteins pathogenic Sclerotinia sclerotiorum remain largely unexplored. Here, we identified novel GPI-anchored protein S. sclerotiorum, SsGSP1, comprising Kre9_KNH domain. SsGSP1 was upregulated during infection, loss-of-function mutants exhibited compromised integrity reduced β-glucan content. During inoculation on Arabidopsis thaliana, Nicotiana benthamiana, Brassica napus, SsGSP1-deletion strains demonstrated decreased virulence. The transgenic A. thaliana line carrying sRNA targeting enhanced resistance via Host-Induced Gene Silencing (HIGS). SsGSP1-deficient displayed heightened sensitivity various stresses, including osmotic pressure, oxidative stress, heat shock. yeast two-hybrid BiFC assays confirmed that interacted with key stress-related catalase SsCat2, shock Sshsp60, ABC transporter SsBMR1. Accordingly, transcriptome analysis revealed disruption downregulated expression genes stress response, chemical agent resistance. These results collectively delineate intricate role β-glucan, virulence, may act potential surface sensor elicit signal transduction response stresses sclerotiorum.

Язык: Английский

Процитировано

0