Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene IW2 DOI Creative Commons

Sujie Yang,

Fan Yang, Zhenglin Yang

и другие.

Plants, Год журнала: 2025, Номер 14(7), С. 1077 - 1077

Опубликована: Апрель 1, 2025

Non-glaucous wheat can reduce solar light reflection in low-light cultivation regions, enhancing photosynthetic efficiency and potentially increasing yield. In previous work, a non-glaucous cuticular line, YL-429, was discovered derivatives of pentaploid hybrids by crossing the synthetic LM/AT23 (non-glaucous cuticular) with its tetraploid donor parent LM (glaucous) selfing to F7 generations. present study, multicolor fluorescence situ hybridization used characterize karyotype YL-429 line; genome resequencing performed identify breakpoint 2D-2A chromosome translocation YL-429; bulk sequencing analysis conducted detect SNP translocated fragment accordingly develop specific kompetitive allele-specific PCR markers for use breeding. The line preliminarily determined as 2DS 2AS (LM T2DS-2AS.2AL) through karyotyping. Genome alignment identified an approximately 13.8 Mb segment, including wax inhibitor gene Iw2, telomeric region arm replacing 16.1 segment that arm. According DNA data, 27 KASP were developed detecting from Aegilops tauschii. T2DS-2AS.2AL could be helpful improving photosynthesis durum cultivated regions. assist screening

Язык: Английский

Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene IW2 DOI Creative Commons

Sujie Yang,

Fan Yang, Zhenglin Yang

и другие.

Plants, Год журнала: 2025, Номер 14(7), С. 1077 - 1077

Опубликована: Апрель 1, 2025

Non-glaucous wheat can reduce solar light reflection in low-light cultivation regions, enhancing photosynthetic efficiency and potentially increasing yield. In previous work, a non-glaucous cuticular line, YL-429, was discovered derivatives of pentaploid hybrids by crossing the synthetic LM/AT23 (non-glaucous cuticular) with its tetraploid donor parent LM (glaucous) selfing to F7 generations. present study, multicolor fluorescence situ hybridization used characterize karyotype YL-429 line; genome resequencing performed identify breakpoint 2D-2A chromosome translocation YL-429; bulk sequencing analysis conducted detect SNP translocated fragment accordingly develop specific kompetitive allele-specific PCR markers for use breeding. The line preliminarily determined as 2DS 2AS (LM T2DS-2AS.2AL) through karyotyping. Genome alignment identified an approximately 13.8 Mb segment, including wax inhibitor gene Iw2, telomeric region arm replacing 16.1 segment that arm. According DNA data, 27 KASP were developed detecting from Aegilops tauschii. T2DS-2AS.2AL could be helpful improving photosynthesis durum cultivated regions. assist screening

Язык: Английский

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