Comparison of different T cell assays for the retrospective determination of SARS-CoV-2 infection DOI
Benjamin A. Krishna, Marina Metaxaki, Marianne Perera

и другие.

Journal of General Virology, Год журнала: 2024, Номер 105(12)

Опубликована: Дек. 20, 2024

It is important to be able retrospectively determine severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections with high accuracy, both for post-coronavirus disease 2019 (COVID-19) epidemiological studies, and distinguish between Long COVID other multi-syndromic diseases that have overlapping symptoms. Although serum antibody levels can measured diagnose SARS-CoV-2 infections, peptide stimulation of memory T cell responses a more sensitive approach. This because robust cells are generated after infection persist even antibodies wane below detectability thresholds. In this study, we compare using FluoroSpot-based methods overnight whole blood peptides followed by an ELISA. Both approaches comparable sensitivity specificity but require different equipment samples used. Furthermore, the elimination cross-react coronaviruses increases assay trades off some sensitivity. Finally, approach used on archival, cryopreserved PBMCs. work shows comparative advantages several measure could utilized any laboratory studying effects pandemic.

Язык: Английский

Comparison of different T cell assays for the retrospective determination of SARS-CoV-2 infection DOI
Benjamin A. Krishna, Marina Metaxaki, Marianne Perera

и другие.

Journal of General Virology, Год журнала: 2024, Номер 105(12)

Опубликована: Дек. 20, 2024

It is important to be able retrospectively determine severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections with high accuracy, both for post-coronavirus disease 2019 (COVID-19) epidemiological studies, and distinguish between Long COVID other multi-syndromic diseases that have overlapping symptoms. Although serum antibody levels can measured diagnose SARS-CoV-2 infections, peptide stimulation of memory T cell responses a more sensitive approach. This because robust cells are generated after infection persist even antibodies wane below detectability thresholds. In this study, we compare using FluoroSpot-based methods overnight whole blood peptides followed by an ELISA. Both approaches comparable sensitivity specificity but require different equipment samples used. Furthermore, the elimination cross-react coronaviruses increases assay trades off some sensitivity. Finally, approach used on archival, cryopreserved PBMCs. work shows comparative advantages several measure could utilized any laboratory studying effects pandemic.

Язык: Английский

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