Traditional Mongolian Medicine Qiqirigan-8 alleviates non-alcoholic fatty liver disease via restoring gut microbiota and metabolism

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 27, 2025

Mongolian Medicine Qiqirigan-8 (MMQ-8) is a traditional medicine formula used to treat fatty liver disease. However, the material basis and in vivo metabolic process of therapeutic effect MMQ-8 on non-alcoholic disease (NAFLD) remain unclear. The chemical composition was determined using Ultra-high-performance liquid chromatography-quadrupole Exactive Mass spectrometry analysis (UHPLC-QE-MS). C57BL/6J mice were fed choline-deficient diet for 12 weeks induce NAFLD model. Hematoxylin Eosin (H&E)-staining, combined with serum biochemical indexes, observe appearance characterize pathological changes functions liver. HE staining Alcian Blue-Phosphoric Acid Schiff (AB-PAS) colon, along ZO-1 immunofluorescence expression colon reveal disruption intestinal epithelial mucosal barrier NAFLD. tight junction genes analyzed by qRT-PCR protective against disruption. Fecal metagenomics non-targeted metabolomics effects gut microbiota metabolism Finally, we emphasize interaction between metabolites through Spearman correlation coefficient analysis. contains 17 active ingredients, which can reduce hepatic steatosis lobular inflammation NAFLD, have injury. reduced infiltration inflammatory cells epithelium model while restoring number goblet cells. significantly enhanced protein as well mRNA both Occludin. results showed that Bacillota/Bacteroidota ratio mice. Increased abundance beneficial bacteria such Porphyromonadaceae, Prevotella, Bacteroidota. suppressed dysfunctional bacteria, Bacillota, Acetatifactor, Erysipelotrichaceae. Furthermore, studies revealed intervention regulated related glutathione metabolism, butyric acid sphingolipid glycerophospholipid compared group. These pathways play key roles According Spearman's analysis, up-regulation Bacteroidota after negatively correlated LPC levels pathways, positively PC levels. In contrast, relationship Bacillota down-regulated intervention, opposite. addition, fumaric acid, 2-oxoglutaric adenosine, L-glutathione levels, those Acetatifactor all above metabolites. Thus, microbial ecosystem are tightly intertwined this process. summary, these findings indicate has synergistic anti-NAFLD its multi-component, multi-target, microbiota-modulating multi pathway characteristics. host's regulation specific involvement multiple may be one important mechanisms exerts It worth noting closely

Язык: Английский

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Obesity alters testicular gene expression in mice, monkeys and humans

Zygote, Год журнала: 2025, Номер unknown, С. 1 - 7

Опубликована: Март 21, 2025

Abstract Obesity, a global health issue, is associated with numerous diseases and has been shown to affect male reproductive by inducing endocrine hormonal changes, chronic inflammation, oxidative stress epigenetic alterations in cells. This study investigates the impact of obesity on testicular gene expression across mice, monkeys humans, identifying 730 conserved testis-specific genes. High-fat diet-induced upregulates GNG5, INHA, MSH5, SLC30A8 SLC7A4 testes, suggesting their potential as regulatory targets damage obesity. Single-cell analysis reveals species-conserved patterns Sertoli cells SPG It also confirmed that were significantly upregulated testes spontaneously obese mice. The findings highlight these genes obesity-related dysfunction, providing insights into impairments caused

Язык: Английский

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Tripeptide DT-109 (Gly-Gly-Leu) attenuates atherosclerosis and vascular calcification in nonhuman primates

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Апрель 7, 2025

Abstract Advanced atherosclerotic lesions and vascular calcification substantially increase the risk of cardiovascular events. However, effective strategies for preventing or treating advanced atherosclerosis are currently lacking. This study investigated efficacy DT-109 (Gly-Gly-Leu) in attenuating nonhuman primates, exploring its broader therapeutic potential. In this study, twenty male cynomolgus monkeys were administered a cholesterol-rich diet ad libitum 10 months. Then, animals treated either orally with (150 mg/kg/day) vehicle (H 2 O) 5 months while continuing on same diet. Plasma lipid levels measured monthly at end experiment, pathological examinations aortas coronary arteries RNA sequencing performed. To explore possible molecular mechanisms, effects smooth muscle cells (SMCs) examined vitro. We found that administration significantly suppressed lesion formation both aorta arteries. Pathological revealed treatment reduced lesional macrophage content calcification. analysis showed downregulated pro-inflammatory factors NLRP3 , AIM2 CASP1 oxidative stress NCF2 NCF4 osteogenic RUNX2 COL1A1 MMP2 MMP9 simultaneously upregulating expression SMCs contraction markers ACTA2 CNN1 TAGLN . Furthermore, inhibited SMC inflammasome activation These results demonstrate effectively suppresses findings, conjunction insights from our previous studies, position as novel multifaceted agent diseases.

Язык: Английский

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Gut microbiota in patients with metabolic, dysfunction-associated steatotic liver disease

Current Opinion in Clinical Nutrition & Metabolic Care, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

Purpose of review Metabolic dysfunction-associated steatotic liver disease (MASLD) is a highly prevalent condition that can progress to fibrosis, steatohepatitis, and hepatocellular carcinoma. This examines recent advances concerning the role gut microbiota in MASLD microbiota-focused interventions positively impact outcome. Recent findings Dysbiotic compromised barrier facilitate translocation microbial-associated molecular patterns harmful metabolites into portal circulation liver, where they exacerbate inflammatory fibrogenic processes. Conversely, other bacterial have protective effects liver. Therefore, homeostasis essential for maintaining health. Summary Levels including ethanol, NH 3 , trimethylamine-L-oxide, 2-oleylglycerol, litocholic acid are often increased patients with MASLD. short-chain fatty acids, indole derivatives, histidine, acids taurodeoxycholic, 3-succinylcholic, hyodeoxycholic decreased. The main aim current interventions/treatments reduce increase beneficial ones. These include drugs (pemafibrate, metformin, obeticholic acid), natural compounds (silymarin, lupeol, dietary fiber, peptides), exogenous bacteria (probiotics, symbionts), special diets (Mediterranean diet, time-restricted feeding), as well transplantation, phage therapy. Most improve permeability, inflammation, fibrosis through regulation, promising alternatives MASLFD management. However, most results come from animal studies, while clinical trials lacking. Further research therefore needed this area.

Язык: Английский

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Mongolian Medicine Qiqirigan-8 Inhibition of P13K/AKT/mTOR Signaling Pathway Activation to Ameliorate Non-alcoholic Steatohepatitis

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2025, Номер unknown, С. 116975 - 116975

Опубликована: Май 1, 2025

Язык: Английский

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