bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 8, 2023
Abstract
Ceramides
are
lipids
that
play
vital
roles
in
complex
lipid
synthesis,
membrane
function,
and
cell
signaling.
Disrupted
ceramide
homeostasis
is
implicated
cell-death
several
neurologic
diseases.
often
analyzed
tissue,
but
this
approach
fails
to
resolve
cell-type
differences
likely
essential
understanding
non-cell
autonomous
contributions
neurodegeneration.
We
show
human
iPSC-derived
neurons
glia
differ
their
rate
of
isoform
composition,
responses
altered
levels.
RNA-sequencing
cells
treated
increase
or
decrease
ceramides
revealed
connections
inflammation,
ER
stress,
apoptosis.
Moreover,
introducing
labeled
sphinganine
showed
readily
synthesize
de
novo
relatively
more
sensitive
toxicity.
Our
findings
provide
a
framework
for
diseases
with
sphingolipid
alternations
insights
designing
therapeutics
target
treating
them.
Cells,
Год журнала:
2024,
Номер
13(19), С. 1655 - 1655
Опубликована: Окт. 5, 2024
Peroxisomes
are
organelles
involved
in
many
cellular
metabolic
functions,
including
the
degradation
of
very-long-chain
fatty
acids
(VLCFAs;
C
≥
22),
initiation
ether-phospholipid
synthesis,
and
metabolism
reactive
oxygen
species.
All
these
processes
essential
for
maintenance
lipid
redox
homeostasis,
their
perturbation
can
trigger
inflammatory
response
immune
cells,
central
nervous
system
(CNS)
resident
microglia
astrocytes.
Consistently,
peroxisomal
disorders,
a
group
congenital
diseases
caused
by
block
biogenesis
or
impairment
one
enzymes,
associated
with
neuroinflammation.
Peroxisomal
function
is
also
dysregulated
neurodegenerative
during
brain
aging,
both
which
This
suggests
that
deciphering
role
peroxisomes
neuroinflammation
may
be
important
understanding
age-related
dysfunction.
In
this
review,
we
discuss
current
advances
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(24), С. 17243 - 17243
Опубликована: Дек. 8, 2023
To
investigate
the
effect
of
therapeutic
treatment
immunopeptide,
peptide
inhibitor
trans-endothelial
migration
(PEPITEM)
on
severity
disease
in
a
mouse
model
experimental
autoimmune
encephalomyelitis
(EAE)
as
for
human
multiple
sclerosis
(MS),
series
experiments
were
conducted.
Using
C57BL/6
female
mice,
we
dosed
PEPITEM
EAE
via
IP
after
observing
first
sign
inflammation.
The
was
induced
using
MOG35-55
and
complete
Freund's
adjuvants
augmented
with
pertussis
toxin.
score
recorded
daily
until
end
experiment
(21
days).
histological
immunohistochemistry
analysis
conducted
spinal
cord
sections.
A
Western
blot
performed
to
measure
protein
concentration
MBP,
MAP-2,
N-Cadherin,
ELISA
kits
used
IL-17
FOXP3
serum
lysate.
reduced
CNS
infiltration
T
cells,
decreased
levels
concertations
N-Cadherin
observed,
addition
FOXP3.
alleviated
symptoms
model.
Our
study
revealed
potential
control
inflammation
MS
patients
reduce
harmful
effects
synthetic
drugs.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 8, 2023
Abstract
Ceramides
are
lipids
that
play
vital
roles
in
complex
lipid
synthesis,
membrane
function,
and
cell
signaling.
Disrupted
ceramide
homeostasis
is
implicated
cell-death
several
neurologic
diseases.
often
analyzed
tissue,
but
this
approach
fails
to
resolve
cell-type
differences
likely
essential
understanding
non-cell
autonomous
contributions
neurodegeneration.
We
show
human
iPSC-derived
neurons
glia
differ
their
rate
of
isoform
composition,
responses
altered
levels.
RNA-sequencing
cells
treated
increase
or
decrease
ceramides
revealed
connections
inflammation,
ER
stress,
apoptosis.
Moreover,
introducing
labeled
sphinganine
showed
readily
synthesize
de
novo
relatively
more
sensitive
toxicity.
Our
findings
provide
a
framework
for
diseases
with
sphingolipid
alternations
insights
designing
therapeutics
target
treating
them.
