Journal of Neuroscience,
Год журнала:
2024,
Номер
44(23), С. e0107242024 - e0107242024
Опубликована: Май 7, 2024
Drugs
of
abuse
cause
changes
in
the
prefrontal
cortex
(PFC)
and
associated
regions
that
impair
inhibitory
control
over
drug-seeking.
Breaking
contingencies
between
drug-associated
cues
delivery
reward
during
extinction
learning
reduces
relapse.
Vagus
nerve
stimulation
(VNS)
has
previously
been
shown
to
enhance
reduce
Here
we
determined
effects
VNS-mediated
release
brain-derived
neurotrophic
factor
(BDNF)
on
cue-induced
reinstatement
male
rats
trained
self-administer
cocaine.
Pairing
10
d
training
with
VNS
facilitated
reduced
drug-seeking
behavior
reinstatement.
Rats
received
a
single
session
showed
elevated
BDNF
levels
medial
PFC
as
via
an
enzyme-linked
immunosorbent
assay.
Systemic
blockade
tropomyosin
receptor
kinase
B
(TrkB)
receptors
extinction,
TrkB
antagonist
ANA-12,
decreased
Whole-cell
recordings
brain
slices
cocaine
self-administration
induced
alterations
ratio
AMPA
NMDA
receptor-mediated
currents
Layer
5
pyramidal
neurons
infralimbic
(IL).
reversed
cocaine-induced
glutamatergic
transmission
by
enhancing
AMPAR
currents,
this
effect
was
blocked
ANA-12.
Our
study
suggests
consolidates
reversing
drug-induced
synaptic
IL,
is
abolished
blocking
highlighting
potential
mechanism
for
therapeutic
addiction.
Physiological Reports,
Год журнала:
2025,
Номер
13(9)
Опубликована: Май 1, 2025
Abstract
The
gastrointestinal
(GI)
tract
plays
a
critical
role
in
nutrient
absorption,
immune
responses,
and
overall
health.
Traditional
models
such
as
two‐dimensional
cell
cultures
have
provided
valuable
insights
but
fail
to
replicate
the
dynamic
complex
microenvironment
of
human
gut.
Gut‐on‐a‐chip
platforms,
which
incorporate
cells
located
gut
into
microfluidic
devices
that
simulate
peristaltic
motion
fluid
flow,
represent
significant
advancement
modeling
GI
physiology
diseases.
This
review
discusses
evolution
gut‐on‐a‐chip
technology,
from
simple
cellular
mono‐cultures
more
sophisticated
systems
incorporating
bi‐cultures
tri‐cultures
enable
studies
drug
metabolism,
disease
modeling,
gut–microbiome
interactions.
Although
challenges
remain,
including
maintaining
long‐term
viability
replicating
these
platforms
hold
great
potential
for
advancing
personalized
medicine
improving
discovery
efforts
targeting
disorders.
Journal of Neuroscience,
Год журнала:
2024,
Номер
44(23), С. e0107242024 - e0107242024
Опубликована: Май 7, 2024
Drugs
of
abuse
cause
changes
in
the
prefrontal
cortex
(PFC)
and
associated
regions
that
impair
inhibitory
control
over
drug-seeking.
Breaking
contingencies
between
drug-associated
cues
delivery
reward
during
extinction
learning
reduces
relapse.
Vagus
nerve
stimulation
(VNS)
has
previously
been
shown
to
enhance
reduce
Here
we
determined
effects
VNS-mediated
release
brain-derived
neurotrophic
factor
(BDNF)
on
cue-induced
reinstatement
male
rats
trained
self-administer
cocaine.
Pairing
10
d
training
with
VNS
facilitated
reduced
drug-seeking
behavior
reinstatement.
Rats
received
a
single
session
showed
elevated
BDNF
levels
medial
PFC
as
via
an
enzyme-linked
immunosorbent
assay.
Systemic
blockade
tropomyosin
receptor
kinase
B
(TrkB)
receptors
extinction,
TrkB
antagonist
ANA-12,
decreased
Whole-cell
recordings
brain
slices
cocaine
self-administration
induced
alterations
ratio
AMPA
NMDA
receptor-mediated
currents
Layer
5
pyramidal
neurons
infralimbic
(IL).
reversed
cocaine-induced
glutamatergic
transmission
by
enhancing
AMPAR
currents,
this
effect
was
blocked
ANA-12.
Our
study
suggests
consolidates
reversing
drug-induced
synaptic
IL,
is
abolished
blocking
highlighting
potential
mechanism
for
therapeutic
addiction.
