Theranostics,
Год журнала:
2024,
Номер
15(4), С. 1524 - 1551
Опубликована: Дек. 31, 2024
Aims:
Lower
vertebrates
and
some
neonatal
mammals
are
known
to
possess
the
ability
regenerate
cardiomyocyte
fully
recover
after
heart
injuries
within
a
limited
period.
Understanding
molecular
mechanisms
of
regeneration
exploring
new
ways
enhance
cardiac
hold
significant
promise
for
therapeutic
intervention
failure.
Sphingosine
1-phospahte
receptor
1
(S1PR1)
is
highly
expressed
in
cardiomyocytes
plays
crucial
role
development
pathological
remodeling.
However,
effect
cardiomyocyte-expressing
S1PR1
on
has
not
yet
been
elucidated.
This
study
aims
investigate
following
injuries.
Methods
Results:
We
generated
(CM)-specific
S1pr1
knock-out
mice
demonstrated
that
CM-specific
loss-of-function
severely
reduced
proliferation
inhibited
apex
resection
mice.
Conversely,
AAV9-mediated
gain-of-function
significantly
enhanced
regeneration.
identified
activated
AKT/mTORC1/CYCLIN
D1
BCL2
signaling
pathways
promote
inhibit
apoptosis.
Moreover,
CM-targeted
gene
delivery
system
via
AAV9
overexpress
increased
improved
functions
myocardial
infarction
adult
mice,
suggesting
potential
method
improve
function
injured
heart.
Conclusions:
demonstrates
CM-S1PR1
an
essential
research
provides
strategy
by
overexpression
as
STAR Protocols,
Год журнала:
2024,
Номер
5(3), С. 103204 - 103204
Опубликована: Июль 21, 2024
Here,
we
present
a
protocol
for
the
quantitative
assessment
of
rat
and
mouse
cardiomyocyte
proliferation
both
in
vitro
vivo.
For
vivo
approach,
describe
steps
isolation
neonatal
cardiomyocytes
employment
various
indicators
to
quantify
cell
proliferation.
We
then
detail
procedures
that
incorporate
comprehensive
assays
genetic
lineage
tracing
strategy
evaluate
endogenous
This
can
be
modified
investigate
other
mammalian
complete
details
on
use
execution
this
protocol,
please
refer
Ji
et
al.
Pharmacological Research,
Год журнала:
2024,
Номер
unknown, С. 107539 - 107539
Опубликована: Дек. 1, 2024
Long
non-coding
RNAs
(lncRNAs)
have
emerged
as
pivotal
regulators
of
cancer
pathogenesis,
influencing
various
cellular
processes
and
contributing
to
tumorigenesis.
Sphingolipid
metabolism
has
garnered
interest
a
potential
target
for
therapy
owing
its
considerable
diagnostic
prognostic
value.
Recent
studies
demonstrated
that
lncRNAs
regulate
tumor-associated
metabolic
reprogramming
via
sphingolipid
metabolism.
However,
the
precise
nature
interactions
between
remains
unclear.
This
review
summarizes
key
roles
in
We
emphasize
interaction
influences
their
impact
on
both
prognosis
drug
resistance.
These
findings
suggest
lncRNA-sphingolipid
holds
great
newl
treatment.
Theranostics,
Год журнала:
2024,
Номер
15(4), С. 1524 - 1551
Опубликована: Дек. 31, 2024
Aims:
Lower
vertebrates
and
some
neonatal
mammals
are
known
to
possess
the
ability
regenerate
cardiomyocyte
fully
recover
after
heart
injuries
within
a
limited
period.
Understanding
molecular
mechanisms
of
regeneration
exploring
new
ways
enhance
cardiac
hold
significant
promise
for
therapeutic
intervention
failure.
Sphingosine
1-phospahte
receptor
1
(S1PR1)
is
highly
expressed
in
cardiomyocytes
plays
crucial
role
development
pathological
remodeling.
However,
effect
cardiomyocyte-expressing
S1PR1
on
has
not
yet
been
elucidated.
This
study
aims
investigate
following
injuries.
Methods
Results:
We
generated
(CM)-specific
S1pr1
knock-out
mice
demonstrated
that
CM-specific
loss-of-function
severely
reduced
proliferation
inhibited
apex
resection
mice.
Conversely,
AAV9-mediated
gain-of-function
significantly
enhanced
regeneration.
identified
activated
AKT/mTORC1/CYCLIN
D1
BCL2
signaling
pathways
promote
inhibit
apoptosis.
Moreover,
CM-targeted
gene
delivery
system
via
AAV9
overexpress
increased
improved
functions
myocardial
infarction
adult
mice,
suggesting
potential
method
improve
function
injured
heart.
Conclusions:
demonstrates
CM-S1PR1
an
essential
research
provides
strategy
by
overexpression
as
