Nanomaterials,
Год журнала:
2024,
Номер
14(24), С. 2017 - 2017
Опубликована: Дек. 15, 2024
Our
group
has
synthesized
a
pleiotropic
synthetic
nanozyme
redox
mediator
we
term
“pleozyme”
that
displays
multiple
enzymatic
characteristics,
including
acting
as
superoxide
dismutase
mimetic,
oxidizing
NADH
to
NAD+,
and
H2S
polysulfides
thiosulfate.
Benefits
have
been
seen
in
acute
chronic
neurological
disease
models.
The
molecule
is
sourced
from
coconut-derived
activated
charcoal
undergone
harsh
oxidization
with
fuming
nitric
acid,
which
alters
the
structure
chemical
yielding
3–8
nm
discs
broad
potential.
Prior
work
showed
pleozymes
localize
mitochondria
increase
oxidative
phosphorylation
glycolysis.
Here,
measured
cellular
NAD+
levels
after
pleozyme
treatment
observed
increased
total
but
not
levels.
A
13C-glucose
metabolic
flux
analysis
suggested
stimulate
generation
of
pyruvate
lactate
glycolytically
tricarboxylic
acid
(TCA)
cycle,
pointing
malate
decarboxylation.
Analysis
intracellular
fatty
abundances
suggests
β-oxidation,
concomitant
succinyl-
acetyl-CoA.
Pleozymes
ATP,
potentially
via
flexible
enhancement
NAD+-dependent
catabolic
pathways
such
glycolysis,
through
TCA
cycle.
These
effects
may
be
favorable
for
pathologies
compromise
metabolism
brain
injury.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 13, 2024
ECHS1
Deficiency
(ECHS1D)
is
a
rare
and
devastating
pediatric
disease
that
currently
has
no
defined
treatments.
This
disorder
results
from
missense
loss-of-function
mutations
in
the
Acta Pharmaceutica Sinica B,
Год журнала:
2024,
Номер
14(12), С. 5181 - 5185
Опубликована: Сен. 27, 2024
Reductive
stress,
characterized
by
rising
level
of
NADH
(nicotinamide
adenine
dinucleotide)
for
a
status
NADH/NAD+
ratio
elevation,
has
been
reported
in
obesity
and
cancer.
However,
the
mechanism
significance
reductive
stress
remain
to
be
established
obesity.
This
perspective
is
prepared
address
issue
with
new
insights
published
recently.
used
production
NADPH,
glutathione,
ATP
heat
classical
biochemistry.
In
obesity,
elevation
ratio,
likely
from
overproduction
due
substrate
overloading,
found
liver
insulin
resistance
gluconeogenesis.
New
evidence
demonstrates
that
may
induce
lipogenesis,
purine
biosynthesis
gluconeogenesis
through
activation
transcription
factors
ChREBP
NRF2.
cancer
cells,
under
Warburg
effect
primarily
derived
decreased
consumption
mitochondrial
respiration.
Alternatively,
NRF2
overactivation
gene
mutation
represents
another
cells.
The
required
quick
proliferation
cells
induction
essential
molecules.
It
appears
causes
are
different
between
cancer,
while
its
impact
anabolism
similar
two
conditions.
Abstract
Identification
of
therapeutic
targets
can
directly
elucidate
the
mechanism
and
effect
drug
therapy,
which
is
a
central
step
in
development.
The
disconnect
between
protein
phenotypes
under
complex
mechanisms
hampers
comprehensive
target
understanding.
Metabolomics,
as
systems
biology
tool
that
captures
phenotypic
changes
induced
by
exogenous
compounds,
has
emerged
valuable
approach
for
identification.
A
overview
was
provided
this
review
to
illustrate
principles
advantages
metabolomics,
delving
into
application
metabolomics
This
outlines
various
metabolomics‐based
methods,
such
dose–response
stable
isotope‐resolved
multiomics,
identify
key
enzymes
metabolic
pathways
affected
substances
through
dose‐dependent
metabolite–drug
interactions.
Emerging
techniques,
including
single‐cell
artificial
intelligence,
mass
spectrometry
imaging,
are
also
explored
their
potential
enhance
discovery.
emphasizes
metabolomics'
critical
role
advancing
our
understanding
disease
accelerating
targeted
development,
while
acknowledging
current
challenges
field.
