Nanomaterials, Год журнала: 2024, Номер 14(24), С. 2017 - 2017

Опубликована: Дек. 15, 2024

Our group has synthesized a pleiotropic synthetic nanozyme redox mediator we term “pleozyme” that displays multiple enzymatic characteristics, including acting as superoxide dismutase mimetic, oxidizing NADH to NAD+, and H2S polysulfides thiosulfate. Benefits have been seen in acute chronic neurological disease models. The molecule is sourced from coconut-derived activated charcoal undergone harsh oxidization with fuming nitric acid, which alters the structure chemical yielding 3–8 nm discs broad potential. Prior work showed pleozymes localize mitochondria increase oxidative phosphorylation glycolysis. Here, measured cellular NAD+ levels after pleozyme treatment observed increased total but not levels. A 13C-glucose metabolic flux analysis suggested stimulate generation of pyruvate lactate glycolytically tricarboxylic acid (TCA) cycle, pointing malate decarboxylation. Analysis intracellular fatty abundances suggests β-oxidation, concomitant succinyl- acetyl-CoA. Pleozymes ATP, potentially via flexible enhancement NAD+-dependent catabolic pathways such glycolysis, through TCA cycle. These effects may be favorable for pathologies compromise metabolism brain injury.

Язык: Английский