bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 26, 2023
Abstract
Clostridioides
difficile
represents
a
major
burden
to
public
health.
As
well-known
nosocomial
pathogen
whose
occurrence
is
highly
associated
with
antibiotic
treatment,
most
examined
C.
strains
originated
from
clinical
specimen
and
were
isolated
under
selective
conditions
emplyoing
antibiotics.
This
suggests
significant
bias
among
analysed
strains,
which
impedes
holistic
view
on
this
pathogen.
In
order
support
extensive
isolation
of
environmental
samples,
we
designed
detection
PCR
that
targets
the
hpdBCA
operon
thereby
identifies
low
abundances
in
samples.
Amplicon-based
analyses
diverse
samples
demonstrated
specific
for
successfully
detected
despite
its
absence
general
16S
rRNA
gene-based
strategies.
Further
revealed
potential
sequence
initial
phylogenetic
classification,
allows
assessing
diversity
via
amplicon
sequencing.
Our
findings
furthermore
showed
treatment
originally
dominated
by
other
according
results.
provided
evidence
cultivation
under-represented
but
antibiotic-resistant
isolates.
Thereby,
substantial
research.
Importance
mainly
responsible
hospital-acquired
infections
after
serious
morbidity
mortality
worldwide.
Research
virulence
focused
bacterial
implies
strains.
Comprehensive
studies
however
require
an
unbiased
strain
collection,
accomplished
avoiding
antibiotic-based
enrichment
Thus,
can
significantly
benefit
our
-specific
PCR,
rapidly
verifies
presence
further
estimation
using
NGS.
eJHaem,
Год журнала:
2024,
Номер
5(3), С. 505 - 515
Опубликована: Апрель 9, 2024
Abstract
Background
Patients
with
hematological
malignancies
(HM)
have
a
high
risk
of
severe
coronavirus
disease
2019
(COVID‐19),
also
in
the
Omicron
period.
Material
and
methods
Retrospective
single‐center
study
including
HM
patients
acute
respiratory
syndrome
Coronavirus
2
(SARS‐CoV2)
infection
from
January
2022
to
March
2023.
Study
outcomes
were
failure
(RF),
mechanical
ventilation
(MV),
COVID‐related
mortality,
comparing
according
SARS‐CoV2
serology.
Results
Note
that,
112
included:
39%
had
negative
Seronegative
older
(71.5
vs.
65.0
years,
p
=
0.04),
more
often
lymphoid
neoplasm
(88.6%
69.1%,
0.02),
underwent
anti‐CD20
therapy
(50.0%
30.9%
0.04)
frequently
(23.0%
3.0%,
0.02)
than
seropositive.
Kaplan‐Meier
showed
higher
for
seronegative
RF
(
0.014),
MV
0.044),
mortality
0.021).
Negative
serostatus
resulted
factor
(hazards
ratio
[HR]
2.19,
95%
confidence
interval
[CI]
1.03–4.67,
(HR
3.37,
CI
1.06–10.68,
4.26,
1.09–16.71,
0.04).
Conclusions
:
serology,
despite
vaccinations
previous
infections,
worse
clinical
compared
seropositive
era.
The
use
serology
diagnosis
could
be
an
easy
tool
identify
prone
developing
complications.
Journal of Infection and Public Health,
Год журнала:
2024,
Номер
17(10), С. 102526 - 102526
Опубликована: Авг. 24, 2024
Patients
with
hematological
malignancies
are
at
increased
risk
of
persistent
coronavirus
disease
2019
(COVID-19)
infection,
a
unique
clinical
condition,
for
which
the
optimal
treatment
is
unknown.
Here
we
report
case
COVID-19
in
acute
lymphoblastic
leukemia
(ALL)
patient
who
successfully
responded
to
extended
course
nirmatrelvir/ritonavir.
Multiple Sclerosis Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 9, 2024
Organizing
pneumonia
(OP),
an
interstitial
lung
disease,
has
been
observed
in
patients
with
inflammatory
demyelinating
diseases
(IDDs)
treated
anti-CD20,
particularly
after
COVID-19,
but
data
are
limited.
medRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 12, 2023
Abstract
Background
Little
data
exists
to
guide
the
treatment
of
persistent
COVID-19
in
immunocompromised
patients.
We
have
employed
a
unique
protocol
combining
tixegavimab/cilgavimab,
and
short-term
combination
antivirals
including
remdesivir.
Methods
A
retrospective
single-center
analysis
Response
was
assessed
by
symptom
resolution,
declining
C-reactive
protein
(CRP)
levels
increasing
SARS-CoV-2-PCR
cycle-threshold
(Ct)
values.
Results
Fourteen
patients
were
included,
2
kidney
transplant
recipients,
11
with
B-cell
lymphoproliferative
disease,
treated
anti-CD20
or
ibrutinib,
1
rheumatoid
arthritis,
anti-CD20.
Median
Ct-value
27
(interquartile
range
(IQR):24-32).
All
received
tixegavimab/cilgavimab
5-day
course
Eleven
also
nirmaltrevir/ritonavir
one
molnupiravir.
follow-up
45
days
(IQR:12-89).
had
complete
responses
decrease
CRP,
increase
Ct
values
(all
either
negative
PCR
value>30
on
day
4-16).
Three
partial
response
relapses
requiring
re-admission.
One
died,
two
responded
prolonged
antiviral
treatments.
Conclusions
monoclonal
antibodies
has
led
resolution
most
severely-immunocompromised
Controlled
studies
will
further
direct
these
patients,
while
more
effective
are
urgently
needed.
Key
points
Some
develop
symptomatic
SARS-CoV-2
infection.
Combination
plus
cured
11/14
Non-responders
benefitted
from
treatment.
trials
needed
find
optimal
COVID-19.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 26, 2023
Abstract
Clostridioides
difficile
represents
a
major
burden
to
public
health.
As
well-known
nosocomial
pathogen
whose
occurrence
is
highly
associated
with
antibiotic
treatment,
most
examined
C.
strains
originated
from
clinical
specimen
and
were
isolated
under
selective
conditions
emplyoing
antibiotics.
This
suggests
significant
bias
among
analysed
strains,
which
impedes
holistic
view
on
this
pathogen.
In
order
support
extensive
isolation
of
environmental
samples,
we
designed
detection
PCR
that
targets
the
hpdBCA
operon
thereby
identifies
low
abundances
in
samples.
Amplicon-based
analyses
diverse
samples
demonstrated
specific
for
successfully
detected
despite
its
absence
general
16S
rRNA
gene-based
strategies.
Further
revealed
potential
sequence
initial
phylogenetic
classification,
allows
assessing
diversity
via
amplicon
sequencing.
Our
findings
furthermore
showed
treatment
originally
dominated
by
other
according
results.
provided
evidence
cultivation
under-represented
but
antibiotic-resistant
isolates.
Thereby,
substantial
research.
Importance
mainly
responsible
hospital-acquired
infections
after
serious
morbidity
mortality
worldwide.
Research
virulence
focused
bacterial
implies
strains.
Comprehensive
studies
however
require
an
unbiased
strain
collection,
accomplished
avoiding
antibiotic-based
enrichment
Thus,
can
significantly
benefit
our
-specific
PCR,
rapidly
verifies
presence
further
estimation
using
NGS.