Cytotoxicity Evaluation of Microbial Sophorolipids and Glucolipids using Normal Human Dermal Fibroblasts (NHDF) in vitro DOI Creative Commons

Sergio Oliveira Formoso,

Vincent Chaleix, Niki Baccile

и другие.

Toxicology Reports, Год журнала: 2024, Номер 14, С. 101862 - 101862

Опубликована: Дек. 11, 2024

Fibroblasts are considered a key player in the wound healing process. Although this cellular family is constituted by several distinct subtypes, dermal fibroblasts crucial thanks to their ability secrete pro-regenerative growth factors, extracellular matrix (ECM) proteins and immune anti-inflammatory role. Sophorolipids (SL), sophorosides (SS) glucolipids (G), mono-unsaturated (C18:1) or saturated (C18:0), glycolipids derived from microbial fermentation of wild type engineered yeast Starmerella bombicola, constitute novel sustainable class bio-based chemicals with interesting physicochemical characteristics, which allow them form soft diverse structures hydrogels vesicles, micelles complex coacervates potential interest skin regeneration applications. In study, we first tested cytocompatibility broad set molecules on normal human (NHDF). Our results show that, up an upper threshold (0.1 % w/v), (SL-C18:1, G-C18:1, SSbola-C18:1, SL-C18:0 G-C18:0) under study were able sustain cell growth. Furthermore, selected least cytotoxic SL-C18:0) promote measuring gene expression markers (i.e. collagen, elastin, transforming factor β, fibroblast …) using qPCR. Unfortunately, none these modulated involved tissue repair. However, aims encourage community test for high-end biomedical Biosurfactants prepared natural amphiphiles growing importance, goal replacing synthetic surfactants commercial formulations. cytotoxicity profile still poorly known, especially new like single-glucose lipids bolaform sophorolipids. This wants contribute all those applications, could be developed biosurfactants contact (cosmetics, healing). We five structurally-related (C18:1 C18:0 sophorolipids, C18:1 lipids, di-sophoroside) against (NHDF) evaluate metabolic activity toxic among them. To best our knowledge, molecules, general, was never NHDF.

Язык: Английский

Bio-Based Surfactants and Biosurfactants: An Overview and Main Characteristics DOI Creative Commons
Gloria Romero Vega, Paola Gallo Stampino

Molecules, Год журнала: 2025, Номер 30(4), С. 863 - 863

Опубликована: Фев. 13, 2025

Natural surfactants are surface-active molecules synthesized from renewable resources (i.e., plants, animals, or microorganisms) and possess properties comparable to conventional surfactants, making them an environmentally friendly potential alternative petrochemical surfactants. Additionally, they exhibit biological such as anti-microbial properties, biodegradability, less toxicity, allowing their use in everyday products with minimal risk human health the environment. Based on mode of production, natural can be classified into first-generation bio-based second-generation biosurfactants, although definition may vary depending author literature. This review offers extensive classification focusing composition, sources, production methods, applications across various industries. Furthermore, main challenges future perspectives discussed.

Язык: Английский

Процитировано

2
Bacillus lipopeptides as key players in rhizosphere chemical ecology DOI Creative Commons
Guillaume Balleux, Monica Höfte, Anthony Argüelles Arias

и другие.

Trends in Microbiology, Год журнала: 2024, Номер 33(1), С. 80 - 95

Опубликована: Авг. 29, 2024

HighlightsCyclic lipopeptides (CLPs) represent unique bacterial natural products with an amazing chemical diversity driving multiple potent bioactivities.More than just lubricants, solubilizers or killing agents, CLPs retain unsuspected array of functions and feature as private tools shared goods produced on demand.Resolving the molecular rules CLP selectivity for a given function requires combined expertise in biology, structural chemistry, biophysics to comprehensively understand their intricate interactions biological membranes.Discovering new should exploit multilevel microbial involve recent advances metagenomics, genome mining, community metabolomicsDeciphering ultimate proximate aspects lipopeptide science highlights relevance ecology.AbstractMicrobial are widely explored therapeutic potential. Understanding underlying evolutionary adaptive forces production remains fundamental question biology. Amphiphilic cyclic (CLPs), prominent category specialized metabolites, show strong antimicrobial activity, particularly against phytopathogens. It is thus assumed that these compounds deployed by soil- rhizosphere-dwelling bacteria weapons competitive environments. Here, we challenge this reductionist perspective present evidence Bacillus mediators ecological interactions. They help communicate, compete, defend predators, cooperate establish mutualistic relationships other (micro)organisms. Additional parallel examples highlighted genera, such Pseudomonas. This broader underscores need further investigation into role shaping strategies key rhizobacterial species.

Язык: Английский

Процитировано

9
Antimicrobial Activity of Bacillus Cyclic Lipopeptides and Their Role in the Host Adaptive Response to Changes in Environmental Conditions DOI Open Access
Н. Н. Маркелова,

Anastasia Chumak

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 336 - 336

Опубликована: Янв. 2, 2025

Bacillus cyclic lipopeptides (CLP), part of the three main families—surfactins, iturins, and fengycins—are secondary metabolites with a unique chemical structure that includes both peptide lipid components. Being amphiphilic compounds, CLPs exhibit antimicrobial activity in vitro, damaging membranes microorganisms. However, concentrations used vitro are difficult to achieve natural conditions. Therefore, environment, alternative mechanisms action by more likely, such as inducing apoptosis fungal cells, preventing microbial adhesion substrate, promoting death phytopathogens stimulating plant immune responses. In addition, low act signaling molecules Bacillus’s own metabolism, when environmental conditions change, they form an adaptive response host bacterium. Namely, trigger differentiation bacterial population into various specialized cell types: competent flagellated matrix producers, spores. this review, we have summarized current understanding under experimental We also shown relationship between some regulatory pathways involved CLP biosynthesis differentiation, well role determine changes physiological state subpopulations shifts

Язык: Английский

Процитировано

1
Fungal membrane determinants affecting sensitivity to antifungal cyclic lipopeptides from Bacillus spp. DOI Creative Commons
Charlotte Bakker,

Hailey R. Graham,

Irina Anca Popescu

и другие.

Fungal Biology, Год журнала: 2024, Номер 128(7), С. 2080 - 2088

Опубликована: Авг. 15, 2024

Bacillus spp. produce numerous antimicrobial metabolites. Among these metabolites, cyclic lipopeptides (CLP) including fengycins, iturins, and surfactins are known to have varying antifungal activity against phytopathogenic fungi. The differential activities of CLP been attributed diverse mechanisms action on fungal membranes. However, the precise biochemical determinants driving their modes not conclusively identified. In this study, three plant pathogenic fungi lipopeptide sensitivities, Alternaria solani, Cladosporium cucumerinum, Fusarium sambucinum, were studied determine how cell membrane lipid compositions may confer sensitivity and/or tolerance fengycin, iturin, surfactin. Results indicated that all correlated with lower ergosterol content elevated phospholipid fatty acid unsaturation. Fungal surfactin was also notably different than fengycin as influenced more by phosphatidylethanolamine amounts, higher levels phosphatidylinositol, less acyl chain length. from study provide insight into composition A. F. C. cucumerinum specific characteristics influencing effectiveness Understanding should enable accurate prediction sensitivity-tolerance outcomes for other species exposed important CLP.

Язык: Английский

Процитировано

4
Ultrasonic preparation, structural characterization and functional enhancement of thyme essential oil-loaded microcapsules based on mannosylerythritol lipid-A DOI Creative Commons

Shunjie Kang,

Qihe Chen, Haorui Ma

и другие.

Ultrasonics Sonochemistry, Год журнала: 2025, Номер 114, С. 107265 - 107265

Опубликована: Фев. 9, 2025

Mannosylerythritol lipid-A (MEL-A) is a kind of novel biosurfactant and has great potential to apply into food pharmaceutical field with its outstanding physicochemical biological property. In this study, Thyme essential oil (TEO) microcapsules based on MEL-A were prepared through ultrasonic emulsification characterized by size, morphology, structure, antioxidant antibacterial activity. The results showed the optimal preparation condition was duration 15 min power intensities 400 W/cm2 ultrasound treatment, improving solubility applicability TEO. Further experiment explored properties activity TEO microcapsules, measuring particle size 276.19 ± 1.72 nm good dispersibility. FT-IR, X-ray, TEM confirmed successful encapsulation within microcapsules. Meanwhile, assayed 7 % exhibited better properties, while those containing 13 performance. conclusion, obvious structural stability functional enhancement in TEO-loaded indicating that applications preservation machinery sterilization are numerous.

Язык: Английский

Процитировано

0
Eco-friendly coating engineered with antimicrobial lipopeptides maintains freshness and induces genes expression in anthocyanin biosynthesis of blueberry DOI

Zihang Cai,

Longyu Wang,

Qiuqin Zhang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141590 - 141590

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Biomimetic surfactants for tunable interfacial properties in drug delivery, biomedical coatings and tissue engineering DOI

Karuppiah Nagaraj,

Ganesh Kumar Anbazhagan,

Rekha Shree Govindasamy

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер 677, С. 125658 - 125658

Опубликована: Апрель 30, 2025

Язык: Английский

Процитировано

0
Phospholipid membrane permeabilization and leakage of cell content by surfactin-C15 from novel B. Subtilis B-11 strain: a computational and experimental analysis DOI
Asma Sani, Jiayi Li,

Chaudhry Haider Ali

и другие.

Опубликована: Июнь 5, 2025

Abstract Surfactin-C15 produced by novel Bacillus Subtilis B-11 strain has potential to inhibit phytopathogens permeabilizing their phospholipid cell membranes at the water/bilayer interface. This permeabilization leads disintegration of membranes, thus inhibiting growth, replication, and pathogenicity phytopathogens. Model dipalmitoyl phosphocholine (DPPC) vesicles for pathogenic were prepared liposomal assays used as representatives bilayer membrane. Results show that hydrophobic fatty acid tail surfactin-C15 binds with acyl chains DPPC membrane rather than hydrophilic head groups which tilt these chains, causing lipid headgroups reorient forming pores in AFM results structural disorderness increases nanoscale, specifically within range 0 3 nm. The fluorescence intensity encapsulated carboxyfluorescein probe a concentration-dependent manner 25 µM, 50 75 measured constant concentration 10 showing an emission increase from 200 800 Heat flow decreases DPPC: (100:0) pretransition temperature Tm 42.2 ± 0.1 (Tonset 40.9 0.1) (10:90) 39.2 36.9 0.1). An cholesterol causes size 240 nm 285 These confirm larger exhibit higher interfacial activity compared smaller vesicles, due greater surface area exposed membrane-water vesicle content is likely cholesterol’s ability modulate fluidity packing, resulting altered morphology. provide more extensive contact molecules interface, facilitating stronger interactions disrupt integrity enhance antimicrobial efficacy. study suggests could be exploited developing major biocontrol strategies agriculture field.

Язык: Английский

Процитировано

0
Biosurfactants and bioamphiphiles, survey, perspectives and applicative potential from a colloid science point of view DOI
Niki Baccile, Jochen Kleinen

Current Opinion in Colloid & Interface Science, Год журнала: 2024, Номер unknown, С. 101891 - 101891

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

2
Deciphering the mechanisms involved in reduced sensitivity to azoles and fengycin lipopeptide in Venturia inaequalis DOI Creative Commons

Aline Leconte,

Justine Jacquin, Matthieu Duban

и другие.

Microbiological Research, Год журнала: 2024, Номер 286, С. 127816 - 127816

Опубликована: Июнь 27, 2024

Apple scab, caused by the hemibiotrophic fungus Venturia inaequalis, is currently most common and damaging disease in apple orchards. Two strains of V. inaequalis (S755 Rs552) with different sensitivities to azole fungicides bacterial metabolite fengycin were compared determine mechanisms responsible for these differences. Antifungal activity tests showed that Rs552 had reduced sensitivity tebuconazole tetraconazole, as well alone or a binary mixture other lipopeptides (iturin A, pumilacidin, lichenysin). S755 was highly sensitive fengycin, whose close tebuconazole. Unlike from iturin family (mycosubtilin, A) similar on both strains, while those surfactin (lichenysin, pumilacidin) not active, except mixtures fengycin. The varies according their structure. Analyses difference azoles (which target CYP51 enzyme involved ergosterol biosynthesis pathway) linked (i) constitutive increased expression Cyp51A gene insertions upstream region (ii) greater efflux membrane pumps involvement ABC transporters. Microscopic observations revealed known interact plasma membranes, induced morphological cytological changes cells strains. Sterol phospholipid analyses higher level ergosta-7,22-dien-3-ol lower PI(C16:0/C18:1) S755. These differences could therefore influence composition explain differential However, antifungal activities mycosubtilin A two indirectly indicate sterols are probably resistance mechanism. This leads conclusion susceptibility studied.

Язык: Английский

Процитировано

1