Meat-derived broth as a novel culture medium for metabolomic study of bacteria in meat DOI Creative Commons
Hye-Jin Kim, Hyun‐Jun Kim, Heesang Hong

и другие.

LWT, Год журнала: 2024, Номер 201, С. 116254 - 116254

Опубликована: Май 28, 2024

Traditional culture media often fail to mimic the natural environment of food-borne bacteria, thereby impeding accurate metabolic profiling. This study developed a novel chicken meat-derived broth (CMB) using various sterilization methods. Metabolic profiles CMBs and bacteria were analyzed UHPLC-Q-Exactive Orbitrap Mass Spectrometry. Among methods tested, filtered pasteurized CMB (FP-CMB) demonstrated highest metabolomic similarity meat. FP-CMB effectively supported microbial growth without inducing changes in turbidity or contamination. The metabolites played role altering energy synthesis glutathione metabolism showcasing distinctions from those observed commercial media. unveils potential as an innovative, representative, efficient medium for microbiological studies, providing precise insights into bacterial metabolomics meat products.

Язык: Английский

Enhancement of highland barley nutrition and flavor via liquid fermentation with Hirsutella sinensis and multi-strains synergistic fermentation DOI
Qingchao Gao, Chen Yang, Huicui Liu

и другие.

Innovative Food Science & Emerging Technologies, Год журнала: 2025, Номер unknown, С. 104059 - 104059

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

The Impact of Biocontrol Agents on the Metabolome of Penicillium nordicum Strains and Its Relation to Ochratoxin A Production on Dry-Cured Ham DOI Creative Commons
Eva Cebrián, Elia Roncero, João Luz

и другие.

Toxins, Год журнала: 2025, Номер 17(5), С. 236 - 236

Опубликована: Май 9, 2025

Throughout the process of dry-cured ham, moulds such as P. nordicum, a producer ochratoxin A (OTA), grow on its surface. The use combined biocontrol agents (BCAs) is promising strategy for controlling this hazard. goal study to assess effect D. hansenii, S. xylosus, and chrysogenum BCAs metabolome two strains nordicum understand differences between both strains. Each ochratoxigenic strain was inoculated individually in combination with onto ham 30 days under environmental conditions experienced during traditional ripening. Untargeted metabolomics performed through mass spectrometry using Q-Exactive Plus Orbitrap. caused alterations metabolomes moulds, mainly phenylalanine catabolism valine, leucine, isoleucine biosynthesis pathways, although some differences. In absence BCAs, types were globally changed, despite these being same species. conclusion, data help us OTA-producing confirm need demonstrate efficacy against wide range toxigenic before they can be used minimise OTA contamination meat industry.

Язык: Английский

Процитировано

0

Metabolomic analysis reveals the positive effects of Rhizopus oryzae fermentation on the nutritional and functional constituents of adlay millet seeds DOI Creative Commons
Caihua Liu, Jian Wei,

Mingde Shi

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 29, 2024

Adlay millet seeds are well known for excellent health benefits. However, using fungal fermentation to improve their nutritional and functional constituents the underlying mechanisms has not been thoroughly investigated. Herein, we used Rhizopus oryzae as starter applied metabolomics combining with quantitative verification understand changes of profiles adlay seeds. Results showed that a total 718 metabolites from 18 compound classes were identified. The R. varied 203 differential metabolites, which 184 became more abundant 19 got less abundant, many components such amino acids, nucleotides, vitamins, flavonoids, terpenoids, phenols significantly increased after process. Interestingly, found synthesized high levels two important beneficial compounds, S-adenosylmethionine (SAMe) β-Nicotinamide mononucleotide (β-NMN), contents 0.56 370.26 μg/g 0.55 8.32 μg/g, respectively. Kyoto Encyclopedia Genes Genomes (KEGG) analysis enriched revealed acid metabolic pathways conversion primary secondary metabolites. Specifically, aspartate can up-regulate biosynthesis SAMe β-NMN. These findings improved our understanding into effects on enhancing values cereal foods.

Язык: Английский

Процитировано

2

Untargeted metabolomics to relate changes produced by biocontrol agents against Aspergillus westerdijkiae and Penicillium nordicum in vitro on dry-cured ham DOI
Eva Cebrián, Elia Roncero, João Luz

и другие.

International Journal of Food Microbiology, Год журнала: 2024, Номер 430, С. 111036 - 111036

Опубликована: Дек. 22, 2024

Язык: Английский

Процитировано

2

Meat-derived broth as a novel culture medium for metabolomic study of bacteria in meat DOI Creative Commons
Hye-Jin Kim, Hyun‐Jun Kim, Heesang Hong

и другие.

LWT, Год журнала: 2024, Номер 201, С. 116254 - 116254

Опубликована: Май 28, 2024

Traditional culture media often fail to mimic the natural environment of food-borne bacteria, thereby impeding accurate metabolic profiling. This study developed a novel chicken meat-derived broth (CMB) using various sterilization methods. Metabolic profiles CMBs and bacteria were analyzed UHPLC-Q-Exactive Orbitrap Mass Spectrometry. Among methods tested, filtered pasteurized CMB (FP-CMB) demonstrated highest metabolomic similarity meat. FP-CMB effectively supported microbial growth without inducing changes in turbidity or contamination. The metabolites played role altering energy synthesis glutathione metabolism showcasing distinctions from those observed commercial media. unveils potential as an innovative, representative, efficient medium for microbiological studies, providing precise insights into bacterial metabolomics meat products.

Язык: Английский

Процитировано

0