ZnO quantum dots reduce acquisition of huanglongbing pathogen by Diaphorina citri

Pest Management Science, Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

Abstract BACKGROUND Diaphorina citri Kuwayama is the main vector of Huanglongbing (HLB) pathogen Candidatus Liberibacter asiaticus ( C Las). Therefore, reducing Las acquisition by D. crucial for controlling spread HLB. RESULTS This study aimed to investigate effect ZnO quantum dots (ZnO QDs) on . The results showed that QDs were absorbed citrus plants and , inducing reactive oxygen species generation in intestine causing intestinal cell apoptosis. uptake rate was reduced 75% 22.3% after treatment with 2 5 mg ∙ mL −1 QDs, respectively, compared 100% control group. Transcription analysis significant changes expression genes related apoptosis, oxidative stress, DNA replication repair. CONCLUSION suggested could efficiently reduce providing a safe reliable tool HLB prevention control. © 2024 Society Chemical Industry.

Язык: Английский

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Evaluation of <em>Artemisia absinthium</em>. L, Essential Oil as a Potential Novel Prophylactic Against the Asian Citrus Psyllid <em>Diaphorina citri</em> Kuwayama

Опубликована: Май 22, 2023

Interest in developing novel crop protectants has gained attention the recent decade due to hazardous effects of synthetic pesticides on humans and environment. The citrus industry worldwide is threatened by D. citri, primary vector phlo-em-limited bacterium (HLB) or greening. However, there no cure for greening available. Diaphorina citri management largely depends insecticides, but their massive application leads resistance pest populations. Therefore, alternative strategies are needed. Our results indicated fewer adults settled plants treated with ABEO than control 48 h after re-lease. psyllid fed leaves significantly secreted less honeydew control. essential oil showed potent ovicidal activity against eggs LC50 5.88 mg/mL. Furthermore, we also explore fitness ABEO-treated untreated Citrus &times; sinensis using two-sex life table tools. that intrinsic rate increase (r) was higher seedlings (0.10 d&minus;1) those a sub-lethal dose (0.07 d&minus;1). Similarly, net re-productive (R0) (14.21 offspring) (6.405 offspring). Notably, were safer Aphis melifera, ranging from 31.05 55.86 mg/mL, which relatively 24.40 mg/mL values citri. indicate exhibits toxic behavioral could be useful managing this pest.

Язык: Английский

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Effect of tree height and spraying methods on Diaphorina citri kuwayama endosymbionts in the context of Huanglongbing disease management in citrus orchards

Pest Management Science, Год журнала: 2023, Номер 80(3), С. 1484 - 1500

Опубликована: Ноя. 10, 2023

Abstract BACKGROUND Huanglongbing (HLB) (caused by Candidatus Liberibacter asiaticus) is the most damaging disease of citrus around world. This study investigated effects tree height on Diaphorina citri Kuwayama mortality, endosymbiont responses, and HLB distribution. RESULTS The results reveal that age trees plays a significant role in psyllid mortality. Interestingly, cumulative mean mortality (%) psyllids over seven‐day observation period was higher (31.50±0.03) when four‐year‐old (501A1, 502A2, 501A3) were sprayed with US‐SMART mechanical sprayer. In contrast, 0.09±0.23 for 13‐year‐old (104A2, 104A3, 104C1) sprayer 9.10±0.05 (502A2, 502B2, 502D1) fixed Our findings also revealed from both four‐ carried Carsonella ruddii species Wolbachia, primary secondary endosymbionts, respectively. Surprisingly, infection rates these endosymbionts remained consistent across different groups, as confirmed quantitative polymerase chain reaction analysis. Furthermore, our highlights significance proxy influencing occurrence. Specifically, subjected to control demonstrated effective management compared sprayers. Additionally, investigation explored impact trees, no correlation between observed, potentially due spray coverage However, sprayer, positive evident. CONCLUSION research provides valuable insights into complex interaction age, These emphasize strategies, especially orchards diverse populations, ultimately contributing long‐term sustainability cultivation. © 2023 Society Chemical Industry.

Язык: Английский

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Grove-level analysis of titer and prevalence of “CandidatusLiberibacter asiaticus” andWolbachiainDiaphorina citri,vector of citrus Huanglongbing

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 6, 2023

Abstract Huanglongbing (HLB, or citrus greening disease) affects all varieties world-wide. In the USA, Asia, and South America causal agent is “ Candidatus Liberibacter asiaticus” ( C Las), a phloem-limited, uncultured, alphaproteobacterium. The hemipteran insect vector, Diaphorina citri (Asian psyllid) acquires transmits Las in circulative, propagative manner. addition to Las, D. hosts multiple symbiotic bacterial species including Wolbachia (wDi). , wDi has been sequenced studied but specific roles biology are unknown. Using well established quantitative PCR methods we measured titer collected from four groves central Florida with distinct HLB management strategies tested whether were correlated sub-set of these insects. Grove site had largest effect on titer. Sex no titer, while higher was non-infected Our results suggest that more directed follow-up research necessary important clarify field tactics influence better understand gene-by-environment interactions among Las. Now millions trees have treated injectable formulations oxytetracycline, which likely decrease populations this study may represent last biologically meaningful snapshot grove-level vector-pathogen ecology state during epidemic.

Язык: Английский

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