Integrative and Comparative Biology,
Год журнала:
2024,
Номер
64(2), С. 565 - 575
Опубликована: Май 17, 2024
Flight
muscle
histolysis
is
a
widespread
strategy
used
by
insects
to
break
down
functional
flight
and
modulate
the
energetic
costs
associated
with
use
maintenance.
The
variable
field
cricket,
Gryllus
lineaticeps,
undergoes
during
their
transition
between
dispersal
reproduction.
Despite
importance
of
on
insect
reproduction
fitness,
molecular
mechanisms
driving
this
breakdown
are
not
well
understood.
Here,
we
show
that
beclin-mediated
autophagy,
conserved
lysosomal-dependent
degradation
process,
drives
dorsal
longitudinal
in
female
flight-capable
G.
lineaticeps.
We
found
lineaticeps
activate
autophagy
(DLM),
but
greater
extent
than
neighboring
dorsoventral
(DVM)
histolysis.
RNA
interference
knockdown
beclin,
gene
encodes
critical
initiation
protein,
delayed
DLM
histolysis,
did
affect
DVM
This
suggests
crickets
selectively
DLMs,
while
maintaining
function
for
other
fitness-relevant
activities
such
as
walking.
Overall,
confirmed
pathway
remodel
cells
providing
novel
insights
into
underlying
major
life
history
Journal of Experimental Biology,
Год журнала:
2024,
Номер
227(12)
Опубликована: Июнь 6, 2024
ABSTRACT
Increased
average
temperatures
and
extreme
thermal
events
(such
as
heatwaves)
brought
forth
by
climate
change
impose
important
constraints
on
aerobic
metabolism.
Notably,
mitochondrial
metabolism,
which
is
affected
both
long-
short-term
temperature
changes,
has
been
put
forward
an
determinant
for
tolerance
of
organisms.
This
study
examined
the
influence
phenotypic
plasticity
metabolic
physiological
parameters
in
Drosophila
melanogaster
link
between
function
their
upper
limits.
We
showed
that
D.
acclimated
to
15°C
have
a
0.65°C
lower
critical
maximum
(CTmax)
compared
with
those
24°C.
exhibited
higher
proportion
shorter
saturated
monounsaturated
fatty
acids,
concomitant
proportions
polyunsaturated
acids.
No
quantitative
changes
(fractional
area
number)
were
detected
acclimation
groups,
but
oxidation
capacities
observed.
Specifically,
15°C-
24°C-acclimated
flies,
complex
I-induced
respiration
was
increased
when
measured
15
24°C,
drastically
declined
at
40°C.
When
succinate
glycerol-3-phosphate
added,
this
decrease
however
compensated
flies
suggesting
impact
related
tolerance.
Our
reveals
use
oxidative
substrates
high
influenced
strongly
difference
CTmax
translates
into
significant
changes.
Genes,
Год журнала:
2023,
Номер
14(12), С. 2177 - 2177
Опубликована: Дек. 4, 2023
Galeruca
daurica
(Joannis)
is
a
pest
species
with
serious
outbreaks
in
the
Inner
Mongolian
grasslands
recent
years,
and
its
larvae
eggs
are
extremely
cold-tolerant.
To
gain
deeper
understanding
of
molecular
mechanism
cold-tolerant
stress
response,
we
performed
de
novo
transcriptome
assembly
G.
via
RNA-Seq
compared
differentially
expressed
genes
(DEGs)
first-
second-instar
grown
developed
indoors
outdoors,
respectively.
The
results
show
that
cold
tolerance
associated
changes
gene
expression
mainly
involved
glycolysis/gluconeogenesis
pathway,
fatty
acid
biosynthesis
pathway
production
heat
shock
proteins
(HSPs).
Compared
control
group
(indoor),
gluconeogenesis,
HSP
were
up-regulated
outdoors.
While
these
related
to
physiological
metabolism
growth
insects,
it
was
hypothesized
encoded
by
play
an
important
role
insects.
In
addition,
also
investigated
metabolic
HSPs,
HSP-related
significantly
outdoors
indoor
group.
Finally,
chose
induce
significant
differences
Hsp70
(Hsp70A1,
Hsp70-2
Hsp70-3)
RNAi
further
illustrate
on
larvae.
separate
mixed
injections
dsHSP70A1,
dsHsp70-2
dsHsp70-3
reduced
levels
target
super-cooling
point
(SCP)
body
fluid
freezing
(FP)
test
determined
after
using
thermocouple
method,
found
silencing
increased
SCP
FP
larvae,
which
validated
resistance
Our
findings
provide
theoretical
basis
for
excavating
key
response
environments
analyzing
adaptation
insects
harsh
environments.
Frontiers in Physiology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 7, 2024
Background:
Many
insect
species
have
evolved
the
ability
to
survive
extracellular
freezing.
The
search
for
underlying
principles
of
their
natural
freeze
tolerance
remains
hampered
by
our
poor
understanding
mechanistic
nature
freezing
damage
itself.
Objectives:
Here,
in
potential
primary
cellular
targets
damage,
we
compared
mitochondrial
responses
(changes
morphology
and
physical
integrity,
respiratory
chain
protein
functionality,
inner
membrane
(IMM)
permeability)
freeze-sensitive
vs.
freeze-tolerant
phenotypes
larvae
drosophilid
fly,
Chymomyza
costata.
Methods:
Larvae
were
exposed
stress
at
-30°C
1
h,
which
is
invariably
lethal
phenotype
but
readily
survived
phenotype.
Immediately
after
melting,
metabolic
activity
muscle
cells
was
assessed
Alamar
Blue
assay,
mitochondria
examined
transmission
electron
microscopy,
functionality
oxidative
phosphorylation
system
measured
Oxygraph-2K
microrespirometry.
Results:
remained
morphologically
functionally
intact
stress.
In
contrast,
most
swollen,
matrix
diluted
enlarged
volume,
structure
IMM
cristae
lost.
Despite
this
morphological
transfer
proteins
partially
functional
lethally
frozen
larvae,
still
exhibiting
strong
specific
substrates
transferring
electrons
oxygen.
However,
coupling
ATP
synthesis
severely
impaired.
Based
on
these
results,
formulated
a
hypothesis
linking
observed
swelling
sudden
loss
barrier
function
IMM.
Insects,
Год журнала:
2024,
Номер
15(7), С. 503 - 503
Опубликована: Июль 5, 2024
Insects’
growth
and
development
are
highly
dependent
on
energy
supply,
with
sugar
metabolism
playing
a
pivotal
role
in
maintaining
homeostasis
regulating
physiological
processes.
The
present
study
investigated
the
effects
of
exendin-4,
glucagon-like
peptide-1
receptor
(GLP-1R)
agonist,
growth,
development,
glycolysis,
fourth-instar
larvae
fall
webworm,
Hyphantria
cunea.
We
determined
impact
exendin-4
larval
nutritional
indices,
analyzed
responses
glycolytic
metabolic
pathways,
revealed
underlying
regulatory
mechanisms.
Exendin-4
treatment
significantly
decreased
influenced
activity
digestive
enzymes,
induced
changes
metabolite
profiles,
particularly
affecting
substance
metabolism.
observed
an
increase
glycogen
content
decrease
glucose
trehalose
levels
hemolymph,
suggesting
effect
blood
homeostasis.
Furthermore,
promoted
glycolysis
by
enhancing
activities
expressions
key
leading
to
pyruvate
production.
This
was
accompanied
reduction
ATP
activation
AMP-activated
protein
kinase
(AMPK),
which
may
underlie
arrest
larvae.
Our
findings
provide
novel
insights
into
insect
from
perspective
contribute
GLP-1R
agonists
for
pest
management.
