Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach DOI
Xiaotong Zhang,

Xuan-Pu Luan,

Jiahang Wei

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

The bean bug Riptortus pedestris is a major soybean pest and cause of the stay-green symptoms. However, molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared antennae transcriptomes starved nonstarved adult R. pedestris, identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed strong response to host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b paralog RpedOR13, with subsequent assays confirming specific 2-PE. Additionally, RNA interference experiments highlighted crucial role detecting Taken together, these findings provide new insights into highlight successful application reverse chemical ecology OR-based screening bioactive compounds.

Язык: Английский

Interactive parallel sex pheromone circuits that promote and suppress courtship behaviors in the cockroach DOI Creative Commons
Kosuke Tateishi, Takayuki Watanabe,

Mana Domae

и другие.

PNAS Nexus, Год журнала: 2024, Номер 3(4)

Опубликована: Март 28, 2024

Abstract Many animals use multicomponent sex pheromones for mating, but the specific function and neural processing of each pheromone component remain unclear. The cockroach Periplaneta americana is a model studying communication, an adult female emits major minor components, periplanone-B -A (PB PA), respectively. Attraction courtship behaviors (wing-raising abdominal extension) are strongly expressed when males exposed to PB weakly they PA. When presented together with PA, elicited by were impaired, indicating that PA can both promote suppress depending on pheromonal context. In this study, we identified receptor genes investigated effects knocking down gene activities PA- PB-responsive sensory neurons (PA- PB-SNs), their postsynaptic interneurons, as well in males. We found activate PB-SNs neurons, activation PB-processing pathway critical expression behaviors. also activates PA-SNs PA-processing pathway. simultaneously presented, undergoes inhibitory control pathway, which weakens Our data indicate physiological interactions between pathways positively negatively mediate attraction pheromones.

Язык: Английский

Процитировано

3
Neurological confirmation of periplanone-D exploitation as a primary sex pheromone and counteractions of other components in the smoky brown cockroach Periplaneta fuliginosa DOI

Mana Domae,

Masazumi Iwasaki,

Hiroshi Nishino

и другие.

Cell and Tissue Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Язык: Английский

Процитировано

0
Biogenic amines and their receptors in insects DOI
Wolfgang Blenau

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Editorial overview: Aroma nudges in bugs: Sensory perception and memory in insects DOI
Makoto Mizunami, Nobuhiro Yamagata

Current Opinion in Insect Science, Год журнала: 2024, Номер 62, С. 101165 - 101165

Опубликована: Янв. 21, 2024

Язык: Английский

Процитировано

0
Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach DOI
Xiaotong Zhang,

Xuan-Pu Luan,

Jiahang Wei

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

The bean bug Riptortus pedestris is a major soybean pest and cause of the stay-green symptoms. However, molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared antennae transcriptomes starved nonstarved adult R. pedestris, identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed strong response to host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b paralog RpedOR13, with subsequent assays confirming specific 2-PE. Additionally, RNA interference experiments highlighted crucial role detecting Taken together, these findings provide new insights into highlight successful application reverse chemical ecology OR-based screening bioactive compounds.

Язык: Английский

Процитировано

0