Pt@ZnCo2O4 Microspheres as Peroxidase Mimics: Enhanced Catalytic Activity and Application for L-Cysteine Detection
Molecules,
Год журнала:
2025,
Номер
30(1), С. 187 - 187
Опубликована: Янв. 5, 2025
Compared
to
natural
enzymes,
the
development
of
efficient
artificial
simulated
such
as
those
based
on
bimetallic
materials
with
high
catalytic
activity
and
good
stability,
is
an
important
way
until
now.
Herein,
we
employed
ZnCo2O4
microspheres
carriers
synthesize
Pt-doped
composites
different
amounts
using
a
one-pot
method.
The
morphology
structure
synthesized
were
characterized
XRD,
SEM,
BET,
FT-IR,
XPS,
Zeta
potential
techniques.
It
was
found
that
Pt0
adhered
well
surface
microspheres,
12.5%
Pt
doped
ratio
exhibiting
abundant
oxygen
vacancies,
excellent
substrate
affinity,
peroxidase-like
activity.
Using
fluorescent
probes
electrochemical
methods,
mechanism
has
been
explored
Pt@ZnCo2O4
can
accelerate
electron
transfer
between
H2O2
3,3',5,5'-tetramethylbenzidine
(TMB).
Based
optimal
loading
Pt@ZnCo2O4,
colorimetric
sensor
for
visual
detection
L-cysteine
(L-Cys)
constructed,
wide
linear
range
0.1~50
µM
low
limit
0.0163
µM.
possesses
selectivity,
reusability,
usage
which
be
applied
determination
L-Cys
in
health
product
capsules
recovery
rates
96.9%~103.7%
RSD
1.07%~6.50%.
This
work
broadens
application
prospects
spinel
field
biological
analysis
also
provides
inspiration
new
enzymes.
Язык: Английский
Research Progress on the Application of Metal Porphyrin Electrochemical Sensors in the Detection of Phenolic Antioxidants in Food
Polymers,
Год журнала:
2025,
Номер
17(6), С. 789 - 789
Опубликована: Март 16, 2025
This
paper
reviews
the
application
of
metal
porphyrin
electrochemical
sensors
in
detection
phenolic
antioxidants
food,
focusing
on
latest
progress
and
innovative
applications
this
field.
Phenolic
are
widely
used
food
can
effectively
prolong
shelf
life
but
their
excessive
use
may
cause
potential
harm
to
human
health,
so
content
is
very
important.
In
recent
years,
analysis
technology
has
gradually
become
an
emerging
method
for
quantitative
due
its
advantages
sensitivity,
simplicity
high
selectivity.
As
a
new
type
sensor,
have
been
excellent
performance
By
modifying
nanomaterials,
these
significantly
improved.
first
introduces
basic
concepts
physicochemical
properties
antioxidants,
analyzes
hazards
discusses
relevant
regulations
limit
requirements.
Then,
existing
methods
compared,
development
trend
traditional
technologies
reviewed.
Subsequently,
discussed
depth,
working
principle
expounded
research
results
summarized.
Finally,
metalloporphyrins
nanocomposites
introduced
detail.
The
unique
highlighted,
future
direction
laid
out.
Язык: Английский
Immobilization of Cholesterol oxidases on functionalized Silica Nanoparticles for biotransformation of cholesterol and 7-ketocholesterol
Shubhrima Ghosh,
Razi Ahmad,
Vikas Kumar Gautam
и другие.
The Journal of Steroid Biochemistry and Molecular Biology,
Год журнала:
2025,
Номер
unknown, С. 106774 - 106774
Опубликована: Май 1, 2025
Cholesterol
oxidation
leads
to
the
development
of
several
oxysterols
such
as
7-ketocholesterol
(7KC),
which
are
linked
various
age-related
conditions.
An
approach
reduce
their
toxicity
is
proposed
using
enzymes
from
microbial
sources
degrade
them.
Our
earlier
studies
identified
Pseudomonas
aeruginosa
PseA
and
Rhodococcus
erythropolis
MTCC
3951
potential
strains
capable
7KC
sole
carbon
source.
These
produced
cholesterol
oxidase
primary
enzyme
in
degradation
pathway.
To
enhance
applicability,
(ChOx)
P.
(ChOxP),
R.
(ChOxR),
a
commercial
variant
Streptomyces
sp.
(ChOxS)
were
immobilized
on
silane
functionalized
silica
nanoparticles
(SNP)
covalent-coupling
methods.
The
immobilization
efficiency
was
68%,
86%,
83%
for
ChOxP,
ChOxR,
ChOxS
respectively.
catalytic
nearly
twice
that
free
enzyme,
with
increased
stability
across
wide
range
temperatures
(10-70°C)
pH
levels
(4.0-9.0),
although
optimum
(7.5)
temperature
(30°C)
remained
unchanged.
nano-immobilized
oxidases
reusable
up
10
cycles.
Further,
confirmed
by
FTIR,
SEM,
TEM.
Biotransformation
nanobioconjugates
pharmaceutically
important
molecules
4-cholesten-3-one
4-cholesten-3,7-dione
Язык: Английский