Journal of Functional Biomaterials,
Год журнала:
2022,
Номер
13(4), С. 242 - 242
Опубликована: Ноя. 15, 2022
Currently,
nanoparticles
and
nanomaterials
are
widely
used
for
biomedical
applications.
In
the
present
study,
silver
(AgNPs)
were
successfully
biosynthesized
using
a
cell-free
extract
(CFE)
of
Bacillus
thuringiensis
MAE
6
through
green
ecofriendly
method.
The
size
AgNPs
was
32.7
nm,
their
crystalline
nature
confirmed
by
XRD,
according
to
characterization
results.
A
surface
plasmon
resonance
spectrum
obtained
at
420
nm.
Nanoparticles
further
characterized
DLS
FTIR
analyses,
which
provided
information
on
size,
stability,
functional
groups.
revealed
less
cytotoxicity
against
normal
Vero
cell
line
[IC50
=
155
μg/mL].
Moreover,
exhibited
promising
antifungal
activity
four
most
common
Aspergillus,
including
Aspergillus
niger,
A.
terreus,
flavus,
fumigatus
concentrations
500
μg/mL
where
inhibition
zones
16,
20,
26,
19
mm,
respectively.
addition,
MICs
125,
62.5,
15.62,
62.5
μg/mL,
Furthermore,
ultrastructural
study
effect
AgNPs,
wall's
integrity
homogeneity
lost;
membrane
had
separated
from
wall
intruded
into
cytoplasm.
conclusion,
CFE
B.
can
be
as
agent
species
causing
Aspergillosis.
RSC Advances,
Год журнала:
2019,
Номер
9(42), С. 24539 - 24559
Опубликована: Янв. 1, 2019
Nanoparticles
(NPs)
are
new
inspiring
clinical
targets
that
have
emerged
from
persistent
efforts
with
unique
properties
and
diverse
applications.
However,
the
main
methods
currently
utilized
in
their
production
not
environmentally
friendly.
With
aim
of
promoting
a
green
approach
for
synthesis
NPs,
this
review
describes
eco-friendly
preparation
biogenic
NPs
known
mechanisms
biosynthesis.
Natural
plant
extracts
contain
many
different
secondary
metabolites
biomolecules,
including
flavonoids,
alkaloids,
terpenoids,
phenolic
compounds
enzymes.
Secondary
can
enable
reduction
metal
ions
to
one-step
synthetic
processes.
Moreover,
using
often
obviates
need
stabilizing
capping
agents
yields
biologically
active
shape-
size-dependent
products.
Herein,
we
formation
metallic
induced
by
natural
list
used
NPs.
In
addition,
use
bacterial
fungal
is
highlighted,
parameters
influence
rate
particle
production,
size,
morphology
discussed.
Finally,
importance
uniqueness
NP-based
products
illustrated,
commercial
applications
various
fields
briefly
featured.
Nanomaterials,
Год журнала:
2020,
Номер
10(6), С. 1146 - 1146
Опубликована: Июнь 11, 2020
A
large
number
of
metallic
nanoparticles
have
been
successfully
synthesized
by
using
different
plant
extracts
and
microbes
including
bacteria,
fungi
viruses
microalgae.
Some
these
showed
strong
antimicrobial
activities
against
phytopathogens.
Here,
we
summarized
green-synthesized
from
plants
their
applications
in
the
control
pathogens.
We
also
discussed
potential
deleterious
effects
on
beneficial
microbial
communities
associated
with
plants.
Overall,
this
review
calls
for
attention
regarding
use
controlling
diseases
clarification
risks
to
plants,
plant-associated
communities,
environments
before
them
agriculture.
PLoS ONE,
Год журнала:
2019,
Номер
14(6), С. e0216496 - e0216496
Опубликована: Июнь 20, 2019
In
this
study,
we
report
on
the
synthesis
of
silver
nanoparticles
(AgNPs)
from
leaf
extracts
Cynara
scolymus
(Artichoke)
using
microwave
irradiation
and
evaluation
its
anti-cancer
potential
with
photodynamic
therapy
(PDT).
Silver
formation
was
characterized
by
scanning
electron
microscopy
energy
dispersive
x-ray
spectroscopy
Fourier
transform
infrared
(FTIR)
spectroscopy.
also
investigated
surface
charge,
particle
size
distribution
zetasizer
analysis.
The
cytotoxic
effect
AgNPs
and/or
PDT
studied
MTT
assay
migration
scratch
assay.
apoptotic
inducing
ability
intracellular
ROS
analysis,
antioxidant
enzyme
levels
(SOD,
CAT,
GPx
GSH),
Hoechst
staining
Bax/Bcl-2
analysis
western
blotting.
mean
produced
found
98.47±2.04
nm
low
polydispersity
(0.301±0.033).
Zeta
values
show
-32.3±
0.8
mV.
These
results
clearly
indicate
successful
for
cellular
uptake.
Mitochondrial
damage
production
were
observed
upon
treatment
(10μg/mL)
(0.5
mJ/cm2)
showed
significant
reducing
cell
migration,
expression
Bax
suppression
Bcl-2.
Significantly,
biosynthesized
a
broad-spectrum
activity
therefore
represent
promoting
generation
modulating
mitochondrial
apoptosis
induction
in
MCF7
breast
cancer
cells.
Molecules,
Год журнала:
2021,
Номер
26(4), С. 844 - 844
Опубликована: Фев. 5, 2021
The
nanomaterial
industry
generates
gigantic
quantities
of
metal-based
nanomaterials
for
various
technological
and
biomedical
applications;
however,
concomitantly,
it
places
a
massive
burden
on
the
environment
by
utilizing
toxic
chemicals
production
process
leaving
hazardous
waste
materials
behind.
Moreover,
employed,
often
unpleasant
can
affect
biocompatibility
generated
particles
severely
restrict
their
application
possibilities.
On
these
grounds,
green
synthetic
approaches
have
emerged,
offering
eco-friendly,
sustainable,
nature-derived
alternative
methods,
thus
attenuating
ecological
footprint
industry.
In
last
decade,
plethora
biological
has
been
tested
to
probe
suitability
synthesis.
Although
most
were
successful,
large
body
evidence
indicates
that
material
or
entity
used
would
substantially
define
physical
chemical
properties
as
consequence,
activities
obtained
nanomaterials.
present
review
provides
comprehensive
collection
recent
methodologies,
surveys
major
nanoparticle
characterization
techniques
screens
effects
triggered
in
living
systems
give
an
impression
potential
synthesized
silver
gold
nanoparticles.
