Decellularized Human Amniotic Member Hydrogel Promotes Limbal Stem Cells Proliferation DOI Creative Commons

Yongyao Tan,

Wei Wang,

Lingjuan Xu

и другие.

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114656 - 114656

Опубликована: Март 1, 2025

Allogeneic cultured limbal epithelial stem cell transplantation has shown variable clinical success in treating deficiency, low cases are likely due to insufficient quantity or functional impairment. In this study, we engineered a decellularized amniotic membrane hydrogel (dAM-gel) using freeze-thaw protocol designed retain extracellular matrix integrity. Post-processing, collagen content decreased modestly from 313.50 ± 27.89 μg/mg 284.8 14.82 (P = 0.08), while glycosaminoglycan levels shifted 7.20 1.66 6.28 0.55 0.27). Crucially, the achieved near-complete DNA removal (7.41 0.78 vs. 0.14 0.06 μg/mg) < 0.0001), ensuring minimal immunogenicity. Although dAM-gel demonstrates limited gelation capacity at lower concentrations, it achieves robust 14 mg/ml, completing process within 28.26 1.21 minutes. Furthermore, facilitates migration and proliferation of cells, particularly p63 + which known correlate with treatments. A plausible explanation for phenomenon is that contains high concentration agrin, cells preserving their stemness via Yap1-cyclin D1 signaling pathway. conclusion, derived presents therapeutic promise deficiency by enhancing maintaining phenotype.

Язык: Английский

Decellularized Human Amniotic Member Hydrogel Promotes Limbal Stem Cells Proliferation DOI Creative Commons

Yongyao Tan,

Wei Wang,

Lingjuan Xu

и другие.

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114656 - 114656

Опубликована: Март 1, 2025

Allogeneic cultured limbal epithelial stem cell transplantation has shown variable clinical success in treating deficiency, low cases are likely due to insufficient quantity or functional impairment. In this study, we engineered a decellularized amniotic membrane hydrogel (dAM-gel) using freeze-thaw protocol designed retain extracellular matrix integrity. Post-processing, collagen content decreased modestly from 313.50 ± 27.89 μg/mg 284.8 14.82 (P = 0.08), while glycosaminoglycan levels shifted 7.20 1.66 6.28 0.55 0.27). Crucially, the achieved near-complete DNA removal (7.41 0.78 vs. 0.14 0.06 μg/mg) < 0.0001), ensuring minimal immunogenicity. Although dAM-gel demonstrates limited gelation capacity at lower concentrations, it achieves robust 14 mg/ml, completing process within 28.26 1.21 minutes. Furthermore, facilitates migration and proliferation of cells, particularly p63 + which known correlate with treatments. A plausible explanation for phenomenon is that contains high concentration agrin, cells preserving their stemness via Yap1-cyclin D1 signaling pathway. conclusion, derived presents therapeutic promise deficiency by enhancing maintaining phenotype.

Язык: Английский

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