Engineering Bacteria for Environmental Release: Regulatory Challenges and Design Strategies

Authorea (Authorea), Год журнала: 2024, Номер unknown

Опубликована: Июнь 25, 2024

Promising many societal benefits, emergent products of biotechnology involve releasing genetically modified microbes (GMMs) into the environment.However, regulatory challenges limit their use.So far, GMMs have mainly been tested in agriculture and environmental cleanup, with few approved for commercial purposes.Current government regulations inadequately address modern genetic engineering potential gut therapeutics, skin products, self-repairing materials, ocean pollution treatment, anti-corrosion coatings, etc.This review first describes current U.S. engineered bacterial release.We analyze 57 global studies on soil-released laboratory microcosm experiments.We discuss behavior released bacteria offer guidelines to improve performance, control persistence, reduce risk.Advanced technologies that GMM function control, but lead increases scrutiny, are reviewed.Finally, we propose a new framework informed by recent data maximize benefits rapidly risks. v Box 1: Commercial releases bacteriaTo our knowledge, these examples only use.The example was not per se, it deployed field outside testing area perform its function.Supplementary Tables 2-3 describe published trials, most those commercially developed.Randomly-mutagenized included.Stopping Crown Gall disease trees antibiotic-producing bacteria.The pathogen Agrobacterium tumefaciens colonizes woody perennials, causing abnormal growth roots branches.It is killed agrocin, produced radiobacter K1026 29 , genes encoded plasmid, raising HGT concerns.Therefore, plasmid tra gene knocked out eliminate conjugative transfer 30 .In Australia 1988, became world's commercially-approved release.It 2000 United States 31 .Image crown gall taken Scot Nelson, creative commons license.Phenol remediated Pseudomonas putida.Phenol potent carcinogen due an 81-day fire at largest oil shale mine U.S.S.R. (now Estonia) 1988.Engineered P. putida containing recombinant phenol-degrading pathway spread remediate 32,33 .The were inoculated enclosures where reduced phenol levels 700-fold.Image showing diagram (6; location started), adapted from ref 34 .Bio-insecticides based Bacillus thuringiensis targeted pest control.B. produces proteins kill insects wild-type strains used bio-insecticide globally 35,36 .These disrupt different pests through variations endotoxins they produce (Cry proteins), strain may optimal combination toxins particular 36 .Mycogen Corporation developed M-Cap cloning Bt toxin B. fluorescens, facilitate use maize crops 37 before field.M-Cap 1990.Other similar developed, including prior release 22,[38][39][40] Stem borers, sensitive M-Cap, International Maize Wheat Improvement Center, license.Nitrogenous fertilizer delivery consortium.Nitrogenous consumes 2% energy major contributor runoff greenhouse gases 41 .Microbes do fixed N when conjunction synthetic unless engineered.In 1990s, Rhizobium meliloti got EPA approval legumes, never commercialized.In 2019, Pivot Bio commercialized Klebsiella variicola deliver nitrogen corn, later consortium adding Kosakonia sacchari.Their product millions acres replaced estimated 32,000 tons 2022 42 courtesy Bio.Engineered subtilis probiotic treating hangovers.Enzyme-based therapeutics often suffer inactivation stored or consumed.Activity could be improved if site needed body.Zbiotic over-thecounter liquid drink contains designed degrade gut-derived acetaldehyde builds up colon after consuming alcohol.The sale nearly 4 million bottles circulation as April 2024.Image Zbiotics.Oral cavity-reducing Streptococcus mutans lactic acid.Biofilms can cause dental cavities producing acid.An isolate S. found antibiotic mutacin 1140, increasing ability outcompete native colonize teeth 43 this strain, lactate dehydrogenase alcohol Zymomonas mobilis, using allelic exchange.The resulting stopped acid 44 .It one-time application cavity formation.While trying approve early 2000s oral therapeutic, FDA put constraints trial which completed.In 2023, Latern Bioworks brought market "cosmetic" avoid need permit.Image decay bacteria.By

Язык: Английский

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Unlocking the efficacy of cement-shell encapsulation for microbial self-healing process of concrete cracks

Journal of Materials Research and Technology, Год журнала: 2024, Номер 32, С. 2733 - 2742

Опубликована: Авг. 23, 2024

Язык: Английский

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Numerical Modeling of the pH Effect on the Calcium Carbonate Precipitation by Sporosarcina pasteurii

Lecture notes in civil engineering, Год журнала: 2025, Номер unknown, С. 141 - 153

Опубликована: Янв. 1, 2025

Язык: Английский

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Self-healing properties of cement-based materials in different matrix based on microbial mineralization coupled with bimetallic hydroxide

Construction and Building Materials, Год журнала: 2023, Номер 400, С. 132686 - 132686

Опубликована: Авг. 5, 2023

Язык: Английский

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Effect of bacteria on uncracked concrete mechanical properties correlated with damage self-healing efficiency – A critical review

Developments in the Built Environment, Год журнала: 2023, Номер 17, С. 100301 - 100301

Опубликована: Дек. 16, 2023

Self-healing concrete has established its effectiveness in repairing cracks, ultimately leading to improved durability. Additionally, there is a need ensure that other crucial mechanical properties, particularly the strength of intact (uncracked) material, remain uncompromised. Despite CaCO3 precipitation being primary component bacterial reaction product responsible for healing cracks and enhancing strength, leveraging effect bacteria on concrete's properties could be subjective, given several factors influence either positive or an adverse manner. Existing reviews topic predominantly focus self-healing performance (post-damage recovery), with none published dedicated, purely, cementitious/concrete material. This paper impact control (intact) mortar under various conditions, without incorporation materials such as fibers, carriers, aggregate replacement, cementitious material replacement. Since concretes strong attributes but poor would unsuitable structural applications, detailed correlation between before after activation discussed. Research gaps are identified, outlook future research presented, along perspective concrete/cementitious production robust properties. The study also contributes addressing one identified – generalized index incorporates both efficiency proposes typical model illustrates application using numerous existing experimental results performance. sheds light overall

Язык: Английский

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Microbial self-healing of cracks in cement-based materials and its influencing factors

Frontiers of Structural and Civil Engineering, Год журнала: 2023, Номер 17(11), С. 1630 - 1642

Опубликована: Ноя. 1, 2023

Язык: Английский

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A state-of-the-art review of the development of self-healing concrete for resilient infrastructure

Frontiers of Structural and Civil Engineering, Год журнала: 2024, Номер 18(2), С. 151 - 169

Опубликована: Фев. 1, 2024

Язык: Английский

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Trends and opportunities for greener and more efficient microbially induced calcite precipitation pathways: a strategic review

Geotechnical Research, Год журнала: 2024, Номер 11(3), С. 161 - 185

Опубликована: Июль 29, 2024

Microbially induced carbonate precipitation (MICP) is extensively used for soil stabilisation, sand erosion control, bioremediation and concrete repair. However, detailed bibliometric studies on MICP are scarce, particularly regarding industrial feedstocks, such as urea ureolytic bacteria calcium chloride, the associated ammonia gas production, which poses challenges large-scale applications. This study reviews 857 articles from Scopus database (2000–2024), using VOSviewer software to visualise data publications, citations, authors, countries, journals, trending keywords research topics. Significant global collaboration evident, led by China USA. The most cited papers discuss microorganisms enhancing properties, basic process, reducing damage, improving groundwater systems impacts of hydrolysis production. Key terms ‘calcium carbonate’, ‘ammonia’ ‘Sporosarcina pasteurii’ central research. Co-occurrence analysis highlights thematic clusters microbial cementation geological features, emphasising multidisciplinary role in tackling environmental problems. review need mitigate impacts, those production energy use, MICP. Life-cycle assessments reveal that while reduces carbon dioxide emissions, it often consumes more energy, necessitating alternative nutrient sources cost-effective practices

Язык: Английский

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A comprehensive review on the strength, durability, and microstructural analysis of bacterial concrete

Structures, Год журнала: 2024, Номер 68, С. 107078 - 107078

Опубликована: Авг. 27, 2024

Язык: Английский

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Recent advances in microbiology in producing self-healing concrete

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 63 - 91

Опубликована: Янв. 1, 2025

Язык: Английский

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