Algorithms,
Год журнала:
2023,
Номер
16(12), С. 571 - 571
Опубликована: Дек. 17, 2023
At
present,
synthetic
biology
applications
are
based
on
the
programming
of
bacteria
with
custom-designed
genetic
circuits
through
application
a
top-down
strategy.
These
programs
that
implement
certain
algorithm,
bacterium
being
agent
or
shell
responsible
for
execution
program
in
given
environment.
In
this
work,
we
study
possibility
instead
synthesized
circuit,
it
is
circuit
itself
which
emerges
as
result
evolution
simulated
an
evolutionary
algorithm.
This
conducted
by
performing
silico
experiments
community
composed
one
species
strain
behaves
pathogenic
against
rest
non-pathogenic
also
part
bacterial
consortium.
The
goal
eradication
agents
bacteria.
results
obtained
suggest
plausibility
design
appropriate
resulting
from
bottom-up
strategy
and
therefore
experimental
feasibility
Biotechnology Advances,
Год журнала:
2024,
Номер
74, С. 108401 - 108401
Опубликована: Июнь 27, 2024
Metabolic
burden
is
defined
by
the
influence
of
genetic
manipulation
and
environmental
perturbations
on
distribution
cellular
resources.
The
rewiring
microbial
metabolism
for
bio-based
chemical
production
often
leads
to
a
metabolic
burden,
followed
adverse
physiological
effects,
such
as
impaired
cell
growth
low
product
yields.
Alleviating
imposed
undesirable
changes
has
become
an
increasingly
attractive
approach
constructing
robust
factories.
In
this
review,
we
provide
brief
overview
engineering,
focusing
specifically
recent
developments
strategies
diminishing
while
improving
robustness
yield.
A
variety
examples
are
presented
showcase
promise
engineering
in
facilitating
design
construction
Finally,
challenges
limitations
encountered
discussed.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 22, 2024
Although
CRISPR-dCas13,
the
RNA-guided
RNA-binding
protein,
was
recently
exploited
as
a
translation-level
gene
expression
modulator,
it
has
still
been
difficult
to
precisely
control
level
due
lack
of
detailed
characterization.
Here,
we
develop
synthetic
tunable
CRISPR
interference
(Tl-CRISPRi)
system
based
on
engineered
guide
RNAs
that
enable
precise
and
predictable
down-regulation
mRNA
translation.
First,
optimize
Tl-CRISPRi
for
specific
multiplexed
repression
genes
at
translation
level.
We
also
show
is
more
suitable
independently
regulating
each
in
polycistronic
operon
than
transcription-level
CRISPRi
(Tx-CRISPRi)
system.
further
engineer
handle
structure
RNA
various
Escherichia
coli
Vibrio
natriegens.
This
applied
increase
production
3-hydroxypropionic
acid
(3-HP)
by
14.2-fold
via
redirecting
metabolic
flux,
indicating
usefulness
this
flux
optimization
microbial
cell
factories
RNA-targeting
machinery.
Microbiology Research,
Год журнала:
2024,
Номер
15(3), С. 1709 - 1727
Опубликована: Авг. 29, 2024
The
broad
range
of
applications
offered
by
synthetic
biology
and
bioengineering
has
revolutionized
the
ability
to
design
redesign
microorganisms
express
specific
functions,
overcoming
limitations
natural
biological
systems.
This
advancement
been
achieved
through
use
mathematical
models
genetic
circuits,
enabling
precise
microbial
communities.
These
are
defined
as
artificially
created
communities
co-cultures
selected
species
that
share
similar
characteristics
environments.
Reprogramming
an
organism
is
carried
out
inserting
which
designed
in
a
controlled
manner
obtain
biotechnological
products
beneficial
humans,
their
health,
environment.
potential
medicine,
bioremediation,
industry,
pharmaceuticals
make
research
promising
field
for
future.
However,
implementation
carries
risks,
such
horizontal
gene
transfer
possible
environmental
impacts.
It
crucial
carefully
evaluate
these
functions
considering
biocontainment
associated
ethical
ecological
implications.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(9), С. 8384 - 8384
Опубликована: Май 7, 2023
Heme
has
a
variety
of
functions,
from
electronic
reactions
to
binding
gases,
which
makes
it
useful
in
medical
treatments,
dietary
supplements,
and
food
processing.
In
recent
years,
whole-cell
system-based
heme
biosynthesis
methods
have
been
continuously
explored
optimized
as
an
alternative
the
low-yield,
lasting,
adverse
ecological
environment
chemical
synthesis
methods.
This
method
relies
on
two
biosynthetic
pathways
microbial
precursor
5-aminolevulinic
acid
(C4,
C5)
three
known
downstream
heme.
paper
reviews
genetic
metabolic
engineering
strategies
for
production
years
by
optimizing
culture
conditions
techniques
different
microorganisms.
Specifically,
we
summarized
analyzed
possibility
using
biosensors
explore
new
perspective
synthetic
biology,
providing
direction
future
exploration.
Green Energy and Environmental Technology,
Год журнала:
2023,
Номер
2
Опубликована: Окт. 25, 2023
The
biodegradation
of
diverse
organic
compounds
plays
a
pivotal
role
in
environmental
sustainability
and
waste
management.
In
recent
years,
genetically
engineered
microbial
cells
have
emerged
as
promising
tool
to
enhance
capabilities.
This
study
presents
an
innovative
approach
produce
amino
acids
nucleic
by
manipulating
genomes,
shedding
light
on
their
relevance
biodegradation.
Through
the
power
genetic
engineering,
can
be
harnessed
efficiently
degrade
wide
range
pollutants,
contributing
cleaner
healthier
environment.
explores
transformative
potential
engineering
unlocking
biodegradative
capacity
highlights
its
significance
production
acids.
By
leveraging
these
advancements,
we
pave
way
towards
sustainable
management
remediation,
ensuring
greener
more
future.
