Chromatin Regulation of Acetic Acid Stress Tolerance by Ino80 in Budding Yeast Saccharomyces cerevisiae

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

Enhanced environmental stress tolerance is important for microbial production of biofuels and biobased chemicals. However, the roles chromatin regulation in bioproduction remain unclear. Here, we explore effects Ino80, core subunit INO80 remodeling complex, on yeast adaptation. We found that deletion led to increased sensitivity budding Saccharomyces cerevisiae acetic acid, which a common inhibitor lignocellulosic biomass also serves as food preservative. Integrated ATAC-seq RNA-seq analyses further showed deleting resulted extensive changes accessibility gene expression cell wall-related genes. Genetic interaction between AHC2, ADA acetyltransferase complex was proved, direct role AHC2 transcription confirmed. These findings benefit development robust strains preservatives by targeting regulation.

Язык: Английский

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Differential global gene transcription of Saccharomyces cerevisiae by zinc sulfate addition under acetic acid stress and identification of novel zinc and stress-responsive genes related to cell wall function

Fungal Biology, Год журнала: 2025, Номер unknown, С. 101573 - 101573

Опубликована: Апрель 1, 2025

Язык: Английский

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The Impact of Selenium on the Physiological Activity of Yeast Cells Saccharomyces cerevisiae ATCC 7090 and Rhodotorula glutinis CCY 20-2-26

Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(1)

Опубликована: Янв. 16, 2025

Background: This study investigated the selenium-binding capacity of biomass two yeast strains, Saccharomyces cerevisiae American Type Culture Collection (ATCC) 7090 and Rhodotorula glutinis CCY 20-2-26. Methods: The studies carried out methods bioaccumulation by biomass. Inorganic selenium was added to culture media as an aqueous solution Na2SeO3 at concentrations ranging from 0 40 mg Se4+/L. Results: addition >0.5 mg/L significantly reduced yield compared with control in case S. cerevisiae. A significant reduction R. observed only doses >30 mg/L. found that for cerevisiae, cultivation should occur 24 h a medium initial concentration 20 achieve most efficient accumulation Under these conditions, could accumulate 4.27 Se4+/g. For red glutinis, optimal binding conditions were achieved cultivating 48 ion strain more resistant high doses, accumulating 7.53 Se4+/L highest tested dose (40 Se4+/L). Selenium supplementation 72 caused changes morphology cells (e.g., increased surface area control). 20–40 after results cells. Conclusions: impacted carotenoid pigment production, levels decreasing increased. Furthermore, range protein content cellular but did not affect intracellular lipid production.

Язык: Английский

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Optimizing lipid production in oleaginous yeasts for sustainable bioenergy: A review of process parameters, cultivation strategies, and machine learning integration

Biomass and Bioenergy, Год журнала: 2025, Номер 197, С. 107810 - 107810

Опубликована: Март 29, 2025

Язык: Английский

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Synthetic Engineering of Microbes for Production of Terpenoid Food Ingredients

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Terpenoids are a class of chemicals comprising many food ingredient chemicals. Synthetic biology and metabolic engineering have been performed to produce microbial cell factories for their production. For improved production various terpenoid ingredients, heterologous synthetic pathways can be optimized at multiple dimensions. Optimizing chassis precursor supply overcoming the host's inherent rigidity crucial enhancing overall efficiency Integrating regulatory circuits facilitate staged programming precise optimization endogenous metabolism. Engineering long-term genetic stability is essential successful scale-up commercial Maximizing in will rely on interdisciplinary tools advance state-of-the-art capabilities streamlined design construction complex genotypes chassis.

Язык: Английский

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Efficiency and transcriptomic analysis reveal the mechanism of fermentation residue biochar and dosage on enhancing food waste high solid bioethanol fermentation

Journal of Cleaner Production, Год журнала: 2025, Номер unknown, С. 145031 - 145031

Опубликована: Фев. 1, 2025

Язык: Английский

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Introduction of human m6Am methyltransferase PCIF1 facilitates the biosynthesis of terpenoids in Saccharomyces cerevisiae

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Апрель 2, 2025

Язык: Английский

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Expanding the Horizons of Saccharomyces cerevisiae: Nutrition, Oenology, and Bioethanol Production

Sustainability, Год журнала: 2024, Номер 16(24), С. 11151 - 11151

Опубликована: Дек. 19, 2024

Specialty Saccharomyces cerevisiae strains have emerged as key contributors to innovations across various industries, offering unique functionalities that extend beyond conventional applications. This review explores the diverse roles of specialty S. in nutrition, winemaking, and bioethanol production. In field yeast biomass serves a sustainable nutrient-dense source proteins, vitamins, bioactive compounds, presenting potential functional food ingredient. can bioaccumulate trace elements like selenium, zinc, chromium, health benefits, but challenges toxicity recovery must be addressed for safe use supplements. enhances flavor profiles, improves fermentation efficiency, reduces undesirable contributing premium wine quality. The novel applications is vast, including development low-alcohol wines, cryotolerant improved at lower temperatures, reduced chemical additives, highlighting its versatility enhancing quality sustainability. Furthermore, plays pivotal role production, with strain selection further improvement leading enhanced yield particularly from lignocellulosic biomass. By examining latest each these areas, this highlights advancing product sectors.

Язык: Английский

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Cyanamide-Inducible Expression of Homing Nuclease I-Scei for Iterative Genome Engineering and Parallel Promoter Characterisation in Saccharomyces Cerevisiae

Опубликована: Янв. 1, 2024

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Язык: Английский

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Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance

Synthetic and Systems Biotechnology, Год журнала: 2024, Номер 9(4), С. 723 - 732

Опубликована: Май 29, 2024

Acetic acid is a common inhibitor present in lignocellulose hydrolysate, which inhibits the ethanol production by yeast strains. Therefore, cellulosic industry requires strains that can tolerate acetic stress. Here we demonstrate overexpressing native arginase-encoding gene, CAR1, renders Saccharomyces cerevisiae tolerance. Specifically, yield increased 27.3% CAR1-overexpressing strain compared to control under 5.0 g/L The global intracellular amino level and compositions were further analyzed, found CAR1 overexpression reduced total content response Moreover, showed ATP improved cell membrane integrity. Notably, demonstrated effect of was independent spermidine proline metabolism, indicates novel mechanisms for enhancing stress Our studies also suggest genetic element be used synthetic biology efficient fuel ethanol.

Язык: Английский

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