Liquid Biopsy in Cancer Management: Integrating Diagnostics and Clinical Applications
Shashwat Pandey,
Preeti Kumari Yadav
Practical Laboratory Medicine,
Год журнала:
2024,
Номер
unknown, С. e00446 - e00446
Опубликована: Дек. 1, 2024
Liquid
biopsy
is
an
innovative,
minimally
invasive
diagnostic
tool
revolutionizing
cancer
management
by
enabling
the
detection
and
analysis
of
cancer-related
biomarkers
from
bodily
fluids
such
as
blood,
urine,
or
cerebrospinal
fluid.
Unlike
traditional
tissue
biopsies,
which
require
procedures,
liquid
offers
a
more
accessible
repeatable
method
for
tracking
progression,
detecting
early-stage
cancers,
monitoring
therapeutic
responses.
The
technology
primarily
focuses
on
analyzing
circulating
tumor
cells
(CTCs),
DNA
(ctDNA),
other
cancer-derived
genetic
materials.
These
provide
critical
information
heterogeneity,
mutation
profiles,
potential
drug
resistance.
In
clinical
practice,
has
demonstrated
its
utility
in
identifying
actionable
mutations,
guiding
personalized
treatment
strategies,
assessing
minimal
residual
disease
(MRD).
While
holds
immense
promise,
challenges
related
to
sensitivity,
specificity,
standardization
remain.
Efforts
optimize
pre-analytical
analytical
processes,
along
with
establishment
robust
regulatory
frameworks,
are
crucial
widespread
adoption.
This
abstract
highlights
transformative
diagnosis,
prognosis,
monitoring,
emphasizing
role
advancing
oncology.
Further
research,
trials,
harmonization
will
be
vital
realizing
full
precision
care.
Язык: Английский
An innovative approach to development of new pyrazolylquinolin-2-one hybrids as dual EGFR and BRAFV600E inhibitors
Molecular Diversity,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 8, 2025
Novel
quinoline-based
derivatives
2a-e
and
4a-j
have
been
designed
synthesized
as
potential
antiproliferative
agents.
The
compounds
were
screened
for
their
activity
against
sixty
cell
lines
according
to
NCI
protocol.
promising
hybrids
4d-g
are
by
MTT
assays
on
three
cancer
lines:
leukemia
(MOLT-4),
lung
(HOP-92),
breast
(T47D),
with
IC50
values
ranging
from
4.982
±
0.2
36.52
1.46
µM
compared
Staurosporine,
compound
4e
being
the
most
effective.
Derivatives
evaluated
inhibitory
EGFR
BRAFV600E.
Compound
exhibited
highest
activities,
of
0.055
0.002
μM
0.068
0.003
BRAFV600E,
reference
drugs
erlotinib
(IC50
0.06
μM)
vemurafenib
0.035
0.001
μM),
respectively.
Cell
cycle
analysis
HOP-92
manifested
that
pre-G1
apoptosis
signaling
took
place
after
treatment.
Docking
simulations
employed
analyze
modes
scores
respect
results
revealed
strong
affinity
both
BRAFV600E
-
3.226
3.474
kcal/mol,
Язык: Английский
Oral Melanoma in Older Adults: Epidemiology, Molecular Landscape, and Treatment Strategies
Pigment Cell & Melanoma Research,
Год журнала:
2025,
Номер
38(3)
Опубликована: Апрель 14, 2025
ABSTRACT
Oral
melanoma
is
an
aggressive
neoplasm
arising
from
melanocytes
in
the
mucosal
epithelium,
accounting
for
0.2%–0.8%
of
all
melanomas.
Unlike
cutaneous
melanoma,
it
not
associated
with
UV
exposure,
and
its
pathogenesis
involves
complex
genetic
molecular
alterations.
This
predominantly
affects
older
adults
(≥
60
years).
Clinically,
lesions
often
present
as
macular
or
nodular
exophytic
growth
pattern,
sometimes
ulcerated,
exhibit
varied
pigmentation.
Diagnosis
further
complicated
by
non‐pigmented
(amelanotic)
variants
that
can
resemble
other
oral
pigmentations.
Wide
surgical
excision
remains
mainstay
treatment,
combined
chemotherapy;
however,
recurrence
distant
metastasis
remain
high.
While
immunotherapy
has
shown
promise
subtypes,
efficacy
uncertain.
Treatment
particularly
challenging
due
to
comorbidities
treatment‐related
morbidity.
review
summarizes
epidemiology,
clinical
features,
current
treatment
strategies
adults.
Key
advances
mechanisms
underlying
this
are
also
outlined.
As
a
strategic
approach,
integrating
screening
into
routine
geriatric
dental
care,
supported
diagnostic
algorithms,
may
improve
early
detection,
prognosis,
survival
outcomes
vulnerable
population.
Язык: Английский
In Silico Analysis of Novel circRNA‐miRNA‐mRNA Axis in BRAFV600E Melanoma: Implications for Primary to Metastasis Transformation and TIME Modulation
The Journal of Gene Medicine,
Год журнала:
2025,
Номер
27(6)
Опубликована: Май 28, 2025
ABSTRACT
Background
The
BRAF
V600E
mutation
drives
metastatic
transition
by
altering
the
tumor
immune
microenvironment
(TIME)
through
transcriptional
and
post‐transcriptional
regulation.
Recent
studies
identify
circular
RNA
(circRNA)
micro‐RNA
(miRNA)
as
key
regulators
in
melanoma.
This
study
uses
silico
analysis
to
explore
circRNA‐miRNA‐mRNA
axis
melanoma
progression.
Methods
We
analyzed
primary
(
n
=
42)
89)
samples
from
TCGA
(SKCM),
including
their
status,
markers
both
types
explored
association
with
cells
within
TIME.
regulatory
were
verified
using
ROC
curve
analysis,
IHC
data
HPA
database,
an
external
dataset.
Further,
was
established
Cytoscape.
Results
Our
comprehensive
identifies
SYK,
HMOX1,
IL33,
CFH,
FGF1,
COL8A1,
ADAMTS3
gene
signatures,
while
VCAM‐1
SNAI2
dominate
prominent
associated
subtype.
Among
infiltrating
group,
macrophages
M1
M2,
CD8
+
T‐cells,
T
regs
show
a
positive
correlation
significant
infiltration
screened
miRNA
targets
sponges,
built
network
involving
two
circRNAs
(hsa‐circ‐0011617
hsa‐circ‐0011623)
three
miRNAs
(hsa‐miR‐1246,
hsa‐miR‐346,
hsa‐miR‐1197)
connected
identified
signature.
Conclusion
Overall,
our
reveals
that
might
have
potential
role
metastasis
BRAF‐mutated
TIME
via
circRNA–miRNA–mRNA
axis.
Язык: Английский
Withaferin-A induced Vimentin S56 phosphorylation dissociates NEDD9 signaling loop to regress progressive metastatic melanoma into lung adenocarcinoma.
Chemico-Biological Interactions,
Год журнала:
2024,
Номер
unknown, С. 111319 - 111319
Опубликована: Ноя. 1, 2024
Язык: Английский
Prognostic Role of Clinicopathological Characteristics and Serum Markers in Metastatic Melanoma Patients Treated with BRAF and MEK Inhibitors
Cancers,
Год журнала:
2024,
Номер
16(17), С. 2981 - 2981
Опубликована: Авг. 27, 2024
Prognostic
studies
can
provide
important
information
about
disease
biology
and
improve
the
use
of
biomarkers
to
optimize
treatment
decisions.
Язык: Английский
Therapeutic Significance of NLRP3 Inflammasome in Cancer: Friend or Foe?
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13689 - 13689
Опубликована: Дек. 21, 2024
Besides
various
infectious
and
inflammatory
complications,
recent
studies
also
indicated
the
significance
of
NLRP3
inflammasome
in
cancer
progression
therapy.
NLRP3-mediated
immune
response
pyroptosis
could
be
helpful
or
harmful
cancer,
depend
on
nature
tumor
microenvironment.
The
activation
increase
surveillance
efficacy
immunotherapy.
It
can
lead
to
removal
cells
by
recruitment
phagocytic
macrophages,
T-lymphocytes,
other
site.
On
hand,
harmful,
as
chronic
inflammation
driven
supports
creating
an
environment
that
facilitates
cell
proliferation,
migration,
invasion,
metastasis.
release
pro-inflammatory
cytokines
such
IL-1β
IL-18
promote
growth
angiogenesis,
while
sustained
may
suppression,
hindering
effective
anti-tumor
responses.
In
this
review
article,
we
discuss
role
inflammasome-mediated
pathophysiology
types;
understanding
is
essential
for
development
innovative
therapeutic
strategies
spread.
Язык: Английский