Neuropeptide-mediated temporal sensory filtering in a primordial nervous system DOI Creative Commons
Livia S. Wyss, Samuel R. Bray,

Bo Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 19, 2024

Abstract Sensory filtering – prioritizing relevant stimuli while ignoring irrelevant ones is crucial for animals to adapt and survive in complex environments. While this phenomenon has been primarily studied organisms with nervous systems, it remains unclear whether simpler also possess such capabilities. Here, we temporal information processing Schmidtea mediterranea , a freshwater planarian flatworm primitive system. Using long-term behavioral imaging oscillatory ultraviolet (UV) light stimulations rhythms matching the timescale of animal’s short-term memory (∼minutes), observed that planarians initially ignored rhythmic oscillations UV intensity but eventually began tracking them after several cycles, demonstrating sensory filtering. We identified two neuropeptides, knockdown which eliminated initial phase led immediate stimulus-tracking, suggesting these neuropeptides mediate an active gating mechanism preventing response transient fluctuations stimuli. Notably, when stimulation was coupled synchronous visible oscillations, tracked combined signals immediately, indicating coherence across modalities can override gating. Our findings demonstrate even simple systems filter mediated by neuropeptides. Unlike classical fast-acting small-molecule neurotransmitters, provide slower, sustained, global form modulation allows more sophisticated control processing. Significance statement show use specific achieve filtering, behavior previously thought require brain architecture. This neuropeptide-mediated reveals fundamental role processing, offering insights into mechanistic evolutionary origins attention-like behaviors.

Язык: Английский

Ultrafast distant wound response is essential for whole-body regeneration DOI Creative Commons
Yuhang Fan,

Chew Chai,

Pengyang Li

и другие.

Cell, Год журнала: 2023, Номер 186(17), С. 3606 - 3618.e16

Опубликована: Июль 21, 2023

Язык: Английский

Процитировано

30
Stem cells (neoblasts) and positional information jointly dominate regeneration in planarians DOI Creative Commons
Xuhui Chen

Heliyon, Год журнала: 2025, Номер 11(2), С. e41833 - e41833

Опубликована: Янв. 1, 2025

Regeneration is the ability to accurately regrow missing body parts. The unparalleled regenerative capacity and incredible tissue plasticity of planarians, both resulting from presence abundant adult stem cells referred as neoblasts, offer a unique opportunity investigate cellular molecular principles underlying regeneration. Neoblasts are capable self-renewal differentiation into desired cell types for correct replacement lost parts after damage. Positional information in muscle governs polarity patterning plan during homeostasis For removal neoblasts disables feats disruption positional results regeneration inappropriate regions, only combination enables Here, I summarize current state field neoblast lineage potential, subclasses specification, roles proper turnover planarians.

Язык: Английский

Процитировано

0
Invertebrate models of nervous system regeneration DOI
Alina Rashid, Lilly M. Ryll, Yuanquan Song

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 105 - 123

Опубликована: Янв. 1, 2025

Процитировано

0
Decoding Stem Cells: An Overview on Planarian Stem Cell Heterogeneity and Lineage Progression DOI Creative Commons
Maria Dolores Molina, Francesc Cebrià

Biomolecules, Год журнала: 2021, Номер 11(10), С. 1532 - 1532

Опубликована: Окт. 17, 2021

Planarians are flatworms capable of whole-body regeneration, able to regrow any missing body part after injury or amputation. The extraordinary regenerative capacity planarians is based upon the presence in adult a large population somatic pluripotent stem cells. These cells, called neoblasts, offer unique system study process cell specification and differentiation vivo. In recent years, FACS-based isolation RNAi functional analyses as well high-throughput approaches such single-cell sequencing have allowed rapid progress our understanding many different aspects neoblast biology. Here, we summarize current knowledge on molecular signatures that define planarian neoblasts heterogeneity, which includes percentage truly guide commitment into lineage-specific progenitor their specific types.

Язык: Английский

Процитировано

26
Cellular diversity and developmental hierarchy in the planarian nervous system DOI Creative Commons
Livia S. Wyss, Samuel R. Bray, Bo Wang

и другие.

Current Opinion in Genetics & Development, Год журнала: 2022, Номер 76, С. 101960 - 101960

Опубликована: Июль 22, 2022

Язык: Английский

Процитировано

14
Pluripotency retention and exogenous mRNA introduction in planarian stem cells in culture DOI Creative Commons
Kai Lei, Wenya Zhang, Jiajia Chen

и другие.

iScience, Год журнала: 2023, Номер 26(2), С. 106001 - 106001

Опубликована: Янв. 20, 2023

Planarians possess naturally occurring pluripotent adult somatic stem cells (neoblasts) required for homeostasis and whole-body regeneration. However, no reliable neoblast culture methods are currently available, hindering mechanistic studies of pluripotency the development transgenic tools. We report robust delivery exogenous mRNAs. identify optimal media short-term maintenance neoblasts

Язык: Английский

Процитировано

8
The known, unknown, and unknown unknowns of cell-cell communication in planarian regeneration DOI Open Access
Jiajia Chen, Kai Lei

动物学研究, Год журнала: 2023, Номер 44(5), С. 981 - 992

Опубликована: Янв. 1, 2023

Planarians are the most primitive bilateral triploblastic organisms. Most planarian species retain mechanisms for whole-body regeneration; example, they can regenerate their cephalic ganglion after complete excision. This powerful regeneration capacity has made planarians model organisms in regenerative research more than 240 years. The mysteries of have been gradually uncovered while newly developed tools and techniques were applied. Exact cell-cell communication long understood to be a prerequisite regeneration. In recent decades, associated at cellular level. During homeostasis regeneration, stem cells (neoblasts) source all new cells. interplay between neoblasts somatic affects identities proportions various tissues during Here, we review discoveries that scientists sought understand communications cell compartments other types planarians, impact on We also highlight challenges future directions research, field consistently contributed valuable knowledge animal

Язык: Английский

Процитировано

7
Adaptive robustness through incoherent signaling mechanisms in a regenerative brain DOI Creative Commons
Samuel R. Bray, Livia S. Wyss,

Chew Chai

и другие.

Cell Reports, Год журнала: 2024, Номер 43(8), С. 114580 - 114580

Опубликована: Авг. 1, 2024

Язык: Английский

Процитировано

2
Regeneration leads to global tissue rejuvenation in aging sexual planarians DOI Creative Commons
Xiaoting Dai, Xinghua Li, Scott D. Pletcher

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 28, 2023

Abstract An ability to delay aging—or reverse the negative effects of aging—could prevent age-related disease and greatly enhance quality life in old age. However, whether it is possible globally physiological aging order extend healthspan unknown. The freshwater planarian Schmidtea mediterranea has been considered “immortal” due its exceptional tissue regeneration capabilities. Here, we report that a sexually reproducing lineage S. exhibits age-associated decline 12 months after birth. Age-associated changes include alterations sensory organs, loss neurons muscle, fertility, impaired motility, but no reduction stem cells at age 3 years. Differential gene expression analysis, comparing young cells, furthermore revealed cell-type-specific transcription as well classical pathways (e.g., insulin signaling). Remarkably, amputation followed by lost tissues led global reversal these changes. Older individuals underwent showed restored youthful patterns expression, cell states, composition rejuvenation whole-animal physiology. Our work reveals naturally evolved solution planaria may provide insights into anti-aging strategies humans.

Язык: Английский

Процитировано

4
A genetic and microscopy toolkit for live imaging of whole-body regeneration inMacrostomum lignano DOI Creative Commons
Richard Nelson Hall, Hongquan Li,

Chew Chai

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 28, 2024

Summary Live imaging of regenerative processes can reveal how animals restore their bodies after injury through a cascade dynamic cellular events. Here, we present comprehensive toolkit for live whole-body regeneration in the flatworm Macrostomum lignan o, including high throughput cloning pipeline, targeted ablation, and advanced microscopy solutions. Using tissue-specific reporter expression, examine various structures regenerate. Enabled by custom, low-cost luminescence/fluorescence microscope, overcome intense stress-induced autofluorescence to demonstrate first application genetic ablation flatworms limited capacity neurons essential role during wound-healing. Finally, build novel open-source tracking microscope continuously image moving throughout week-long process regeneration, quantifying kinetics wound healing, nerve cord repair, body growth, behavioral recovery. Our findings suggest that reconnection operates independently from primary axis re-establishment other downstream processes.

Язык: Английский

Процитировано

1