Proactive Resistance Management Studies Highlight the Role of Cytochrome P450 Genes in the Resistance of Tuta absoluta Against Tetraniliprole DOI Open Access
Farman Ullah, Zeeshan Ullah, Hina Gul

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5180 - 5180

Опубликована: Май 28, 2025

The diamide insecticide tetraniliprole is a valuable tool for managing major insect pests like the invasive tomato pinworm, Tuta absoluta (Meyrick). However, mechanisms underlying resistance, as well its associated fitness costs, remain unclear. In this study, we assessed of tetraniliprole-resistant (TetraRS) and susceptible (SS) strains T. conducted Illumina RNA-seq to compare their transcriptomes. We also used nanocarrier-mediated RNA interference (RNAi) knockdown P450 genes evaluate role in resistance. After eight generations selection, developed 20.80-fold resistance tetraniliprole, accompanied by costs. analysis revealed 3332 differentially expressed (DEGs), with 1707 upregulated 1625 downregulated TetraRS compared SS strain. Gene Ontology (GO) annotations showed significant enrichment categories related metabolic processes, cellular catalytic activity, anatomical entity, binding. These were identified key KEGG pathways such cytochrome P450, drug metabolism, carbon oxidative phosphorylation, fatty acid protein processing. RT-qPCR confirmed that (CYP405D1, CYP6AB269, CYP4AU1) insects, line results. Cytochrome activity was significantly higher strain than Notably, nano-encapsulated dsRNA targeting these overexpressed increased susceptibility tetraniliprole. Further, reduced following silencing genes. findings suggest multiple pathways, particularly genes, contribute absoluta. This study provides insights into molecular pest species.

Язык: Английский

Risk assessment, fitness cost and transcriptome analysis of cyantraniliprole resistance in Spodoptera frugiperda DOI
Jiaping Wei,

Shushu Lv,

Daojie Guan

и другие.

Pesticide Biochemistry and Physiology, Год журнала: 2024, Номер 203, С. 106000 - 106000

Опубликована: Июнь 21, 2024

Язык: Английский

Процитировано

6
Efficacy of the anthranilic diamide tetraniliprole against four coleopteran pests of stored grain DOI Creative Commons
Waqas Wakil, Nickolas G. Kavallieratos,

Aqsa Naeem

и другие.

Crop Protection, Год журнала: 2025, Номер unknown, С. 107200 - 107200

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Proactive Resistance Management Studies Highlight the Role of Cytochrome P450 Genes in the Resistance of Tuta absoluta Against Tetraniliprole DOI Open Access
Farman Ullah, Zeeshan Ullah, Hina Gul

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5180 - 5180

Опубликована: Май 28, 2025

The diamide insecticide tetraniliprole is a valuable tool for managing major insect pests like the invasive tomato pinworm, Tuta absoluta (Meyrick). However, mechanisms underlying resistance, as well its associated fitness costs, remain unclear. In this study, we assessed of tetraniliprole-resistant (TetraRS) and susceptible (SS) strains T. conducted Illumina RNA-seq to compare their transcriptomes. We also used nanocarrier-mediated RNA interference (RNAi) knockdown P450 genes evaluate role in resistance. After eight generations selection, developed 20.80-fold resistance tetraniliprole, accompanied by costs. analysis revealed 3332 differentially expressed (DEGs), with 1707 upregulated 1625 downregulated TetraRS compared SS strain. Gene Ontology (GO) annotations showed significant enrichment categories related metabolic processes, cellular catalytic activity, anatomical entity, binding. These were identified key KEGG pathways such cytochrome P450, drug metabolism, carbon oxidative phosphorylation, fatty acid protein processing. RT-qPCR confirmed that (CYP405D1, CYP6AB269, CYP4AU1) insects, line results. Cytochrome activity was significantly higher strain than Notably, nano-encapsulated dsRNA targeting these overexpressed increased susceptibility tetraniliprole. Further, reduced following silencing genes. findings suggest multiple pathways, particularly genes, contribute absoluta. This study provides insights into molecular pest species.

Язык: Английский

Процитировано

0