Lipo-Chitooligosaccharides Induce Specialized Fungal Metabolite Profiles That Modulate Bacterial Growth

mSystems, Год журнала: 2022, Номер 7(6)

Опубликована: Дек. 1, 2022

The activation of silent biosynthetic gene clusters (BGC) for the identification and characterization novel fungal secondary metabolites is a perpetual motion in natural product discoveries. Here, we demonstrated that one best-studied symbiosis signaling compounds, lipo-chitooligosaccharides (LCOs), play role activating some these BGCs, resulting production known, putative, unknown with biological activities.

Язык: Английский

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Ecology shapes the genomic and biosynthetic diversification of Streptomyces bacteria from insectivorous bats

Microbial Genomics, Год журнала: 2024, Номер 10(4)

Опубликована: Апрель 16, 2024

Streptomyces are prolific producers of secondary metabolites from which many clinically useful compounds have been derived. They inhabit diverse habitats but rarely reported in vertebrates. Here, we aim to determine what extent the ecological source (bat host species and cave sites) influence genomic biosynthetic diversity bacteria. We analysed draft genomes 132 isolates sampled 11 insectivorous bats six sites Arizona New Mexico, USA. delineated 55 based on genome-wide average nucleotide identity core genome phylogenetic tree. that colonize same bat or site exhibit greater overall similarity than they do with other sites. However, when considering gene clusters (BGCs) alone, BGC distribution is not structured by geographical carry them. Each carried between 19–65 BGCs (median=42.5) varied even among members species. Nine major classes were detected ten all sites: terpene, non-ribosomal peptide synthetase, polyketide synthase, siderophore, RiPP-like, butyrolactone, lanthipeptide, ectoine, melanin. Finally, multiple hybrid consisting signature domains two seven distinct classes. Taken together, our results bring critical insights understanding -bat ecology may contribute health augmenting current efforts natural product discovery, especially underexplored overlooked environments.

Язык: Английский

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Combined effect of simulated microgravity and low-dose ionizing radiation on structure and antibiotic resistance of a synthetic community model of bacteria isolated from spacecraft assembly room

Life Sciences in Space Research, Год журнала: 2023, Номер 38, С. 29 - 38

Опубликована: Апрель 30, 2023

Язык: Английский

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Development of an Experimental Approach to Achieve Spatially Resolved Plant Root-Associated Metaproteomics Using an Agar-Plate System

Molecular Plant-Microbe Interactions, Год журнала: 2022, Номер 35(8), С. 639 - 649

Опубликована: Март 29, 2022

Plant-microbe interactions in the rhizosphere play a vital role plant health and productivity. The composition function of root-associated microbiomes is strongly influenced by their surrounding environment, which often customized host. How change with respect to space time across roots remains poorly understood, methodologies that facilitate spatiotemporal metaproteomic studies are yet be realized. Here, we developed method provides spatially resolved metaproteome measurements along embedded agar-plate culture systems, have long been used study plants. Spatially defined agar "plugs" interest were excised subsequently processed using novel peptide extraction prior metaproteomics, was infer both microbial community function. As proof-of-principle, previously studied 10-member constructed from Populus root system grown an plate 3-week-old trichocarpa plant. Metaproteomics performed two points (24 48 h) for three distinct locations (root base, tip, region distant root). spatial resolution these evidence microbiome expression changes interface. Interrogation individual proteomes revealed functional profiles related behavioral associations root, chemotaxis augmented metabolism likely supported predominance most abundant member. This demonstrated studying unsuitable (meta)proteomic measurements.

Язык: Английский

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Identification and functional characterization of effector proteins from the fungal soil-borne pathogens Verticillium dahliae and Rosellinia necatrix

Опубликована: Янв. 1, 2024

Plant pathogens secrete effector molecules during host invasion to promote colonization.However, some of these effectors become recognized by receptors mount a defence response and establish immunity.Recently, novel resistance was identified in wild tomato, mediated the single dominant V2 locus, control strains soilborne vascular wilt fungus Verticillium dahliae that belong race 2. With comparative genomics 2 resistance-breaking 3 strains, we avirulence activates resistance, termed Av2.We 277 kb 2-specific sequence comprising only two genes encoding predicted secreted proteins are expressed tomato colonization.Subsequent functional analysis based on genetic complementation into isolates targeted deletion from 1 isolate JR2 TO22 confirmed one candidates encodes Av2 is plants.Two allelic variants were encode differ acid.Thus far, role virulence could not be demonstrated for either variants.populations as their deployment monoculture-based cropping systems selects pathogen overcome immunity (Stukenbrock McDonald, 2008;Dangl et al., 2013).Such breaking occurs upon purging Avr gene, diversification, or employment subvert immune (Stergiopoulos 2007;Cook 2015).The molecular cloning first bacterial gene Pseudomonas syringae pv.glycinea reported 1984 (Staskawicz 1984), fungal

Язык: Английский

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