Journal of the Franklin Institute, Год журнала: 2022, Номер 359(8), С. 3779 - 3807
Опубликована: Март 4, 2022
Язык: Английский
Diagnostics, Год журнала: 2023, Номер 13(20), С. 3246 - 3246
Опубликована: Окт. 18, 2023
Antibiotic resistance is a global public health concern, posing significant threat to the effectiveness of antibiotics in treating bacterial infections. The accurate and timely detection antibiotic-resistant bacteria crucial for implementing appropriate treatment strategies preventing spread resistant strains. This manuscript provides an overview current emerging technologies used bacteria. We discuss traditional culture-based methods, molecular techniques, innovative approaches, highlighting their advantages, limitations, potential future applications. By understanding strengths limitations these technologies, researchers healthcare professionals can make informed decisions combating antibiotic improving patient outcomes.
Язык: Английский
Процитировано
53
The ISME Journal, Год журнала: 2022, Номер 17(1), С. 21 - 35
Опубликована: Сен. 23, 2022
Abstract A debate is currently ongoing as to whether intensive livestock farms may constitute reservoirs of clinically relevant antimicrobial resistance (AMR), thus posing a threat surrounding communities. Here, combining shotgun metagenome sequencing, machine learning (ML), and culture-based methods, we focused on poultry farm connected slaughterhouse in China, investigating the gut microbiome livestock, workers their households, microbial communities carcasses soil. For both resistomes this study, differences are observed across environments hosts. However, at finer scale, several similar genes (ARGs) associated mobile genetic elements were found human broiler chicken samples. Next, Escherichia coli, an important indicator for surveillance AMR farm. Strains E. coli intermixed between humans chickens. We that ARGs present faecal resistome showed correlation resistance/susceptibility profiles isolates cultured from same Finally, by using environmental sensing these be correlated variations temperature humidity. Our results show importance adopting multi-domain multi-scale approach when studying complex, interconnected environments.
Язык: Английский
Процитировано
42
Antibiotics, Год журнала: 2023, Номер 12(11), С. 1580 - 1580
Опубликована: Окт. 30, 2023
Recent advancements in sequencing technology and data analytics have led to a transformative era pathogen detection typing. These developments not only expedite the process, but also render it more cost-effective. Genomic analyses of infectious diseases are swiftly becoming standard for analysis control. Additionally, national surveillance systems can derive substantial benefits from genomic data, as they offer profound insights into epidemiology emergence antimicrobial-resistant strains. Antimicrobial resistance (AMR) is pressing global public health issue. While clinical laboratories traditionally relied on culture-based antimicrobial susceptibility testing, integration AMR holds immense promise. Genomic-based furnish swift, consistent, highly accurate predictions phenotypes specific strains or populations, all while contributing invaluable surveillance. Moreover, genome assumes pivotal role investigation hospital outbreaks. It aids identification infection sources, unveils genetic connections among isolates, informs strategies The One Health initiative, with its focus intricate interconnectedness humans, animals, environment, seeks develop comprehensive approaches disease surveillance, control, prevention. When integrated epidemiological systems, forecast expansion bacterial populations species transmissions. Consequently, this provides evolution relationships pathogens, hosts, environment.
Язык: Английский
Процитировано
29
Journal of Biomolecular Structure and Dynamics, Год журнала: 2023, Номер 42(10), С. 5178 - 5196
Опубликована: Июнь 20, 2023
The cases of bacterial multidrug resistance are increasing every year and becoming a serious concern for human health. Multidrug efflux pumps key players in the formation antibiotic resistance, which transfer out broad spectrum drugs from cell convey to host. Efflux have significantly reduced efficacy previously available armory, thereby frequency therapeutic failures. In gram-negative bacteria, AcrAB-TolC pump is principal transporter substrate plays major role resistance. current work, advanced computer-aided drug discovery approaches were utilized find hit molecules library biogenic chalcones against AcrB pump. results performed computational studies
Язык: Английский
Процитировано
18
Journal of Biomolecular Structure and Dynamics, Год журнала: 2023, Номер 42(16), С. 8427 - 8440
Опубликована: Авг. 11, 2023
Antibiotic resistance has become a pressing global health crisis, with bacterial infections increasingly difficult to treat due the emergence of multidrug resistance. This study aims identify potential chalcone molecules that interact two key efflux pumps, AcrB and EmrD, Escherichia coli, using advanced computational tools.
Язык: Английский
Процитировано
11
Microorganisms, Год журнала: 2022, Номер 10(12), С. 2362 - 2362
Опубликована: Ноя. 29, 2022
Over the past few decades, antimicrobial resistance (AMR) has emerged as an important threat to public health, resulting from global propagation of multidrug-resistant strains various bacterial species. Knowledge intrinsic factors leading this is necessary overcome these new strains. This contributed increased use omics technologies and their extrapolation system level. Understanding mechanisms involved in acquired by microorganisms at level essential obtain answers explore options combat resistance. Therefore, robust whole-genome sequencing approaches other techniques such transcriptomics, proteomics, metabolomics provide fundamental insights into physiology To improve efficiency data obtained through approaches, thus gain a predictive understanding responses antibiotics, integration mathematical models with genome-scale metabolic (GEMs) essential. In context, here we outline recent efforts that have demonstrated technology systems biology, quantitative hypothesis-generating frameworks, can antibiotic resistance, it hoped emerging field support for efforts.
Язык: Английский
Процитировано
18
Bioinformatics Advances, Год журнала: 2022, Номер 2(1)
Опубликована: Янв. 1, 2022
With the steadily increasing abundance of omics data produced all over world under vastly different experimental conditions residing in public databases, a crucial step many data-driven bioinformatics applications is that integration. The challenge batch-effect removal for entire databases lies large number batches and biological variation, which can result design matrix singularity. This problem currently not be solved satisfactorily by any common batch-correction algorithm.We present reComBat, regularized version empirical Bayes method to overcome this limitation benchmark it against popular approaches harmonization gene-expression (both microarray bulkRNAsq) human opportunistic pathogen Pseudomonas aeruginosa. Batch-effects are successfully mitigated while biologically meaningful variation retained. reComBat fills gap applicable large-scale, opens up new avenues analysis complex processes beyond scope single study.The code available at https://github.com/BorgwardtLab/reComBat, evaluation found https://github.com/BorgwardtLab/batchCorrectionPublicData.Supplementary Bioinformatics Advances online.
Язык: Английский
Процитировано
17
Journal of Advanced Research, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
3
Healthcare, Год журнала: 2023, Номер 11(9), С. 1345 - 1345
Опубликована: Май 7, 2023
Nosocomial pneumonia is one of the most frequent hospital-acquired infections. One types nosocomial ventilator-associated pneumonia, which occurs in endotracheally intubated patients intensive care units (ICU). Ventilator-associated may be caused by multidrug-resistant pathogens, increase risk complications due to difficulty treating them. Pneumonia a respiratory disease that requires targeted antimicrobial treatment initiated as early possible have good outcome. For therapy specific and started sooner, diagnostic methods evolved rapidly, becoming quicker simpler perform. Polymerase chain reaction (PCR) rapid technique with numerous advantages compared classic plate culture-based techniques. Researchers continue improve methods; thus, newest PCR can performed at bedside, ICU, so-called point testing—PCR (POC-PCR). The purpose this review highlight benefits drawbacks PCR-based techniques managing pneumonia.
Язык: Английский
Процитировано
8
Microbial Biotechnology, Год журнала: 2023, Номер 16(10), С. 1900 - 1923
Опубликована: Июль 7, 2023
Abstract As recognized by several international agencies, antibiotic resistance is nowadays one of the most relevant problems for human health. While this problem was alleviated with introduction new antibiotics into market in golden age antimicrobial discovery, few are pipeline. Under these circumstances, a deep understanding on mechanisms emergence, evolution and transmission resistance, as well consequences bacterial physiology acquiring needed to implement novel strategies, beyond development or restriction use current ones, more efficiently treat infections. There still aspects field that not fully understood. In article, we make non‐exhaustive critical review some them consider special relevance, aim presenting snapshot studies need be done tackle resistance.
Язык: Английский
Процитировано
8