Perspective Chapter: Molecular Diagnostics in Viral Outbreak Surveillance DOI
Jennifer Giandhari, Amsha Viraragavan, Michelle Gordon

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Understanding and adopting various methods for monitoring viral outbreaks is required pathogen surveillance. Accurate diagnosis can play a significant role in the safe effective treatment prescribed. Africa remains burdened with host of infectious diseases, which challenges healthcare systems proper management diseases. Surveillance are implemented some parts that have infrastructure funding to perform routine testing screening. However, not all this continent equipped necessary tools support. With travel, tourism goods exchange, diseases spread across borders rapidly, posing threat global health, emphasising unified need efforts prevent, detect, act on disease through improved access vaccinations treatments. Effective response depends several elements, including laboratory capacity, skilled health staff, surveillance detect limit illnesses rapidly. Traditional molecular such as genotyping polymerase chain reaction (PCR)-based detection now being complemented like next-generation sequencing clustered regularly interspaced short palindromic repeats (CRISPR). This book chapter aims summarise current broadly describes issue climate change its impact outbreaks.

Язык: Английский

Emerging Trends in Antimicrobial Resistance in Polar Aquatic Ecosystems DOI Creative Commons
Melissa Bisaccia, Francesca Berini, Flavia Marinelli

и другие.

Antibiotics, Год журнала: 2025, Номер 14(4), С. 394 - 394

Опубликована: Апрель 10, 2025

The global spread of antimicrobial resistance (AMR) threatens to plummet society back the pre-antibiotic era through a resurgence common everyday infections’ morbidity. Thus, studies investigating antibiotic genes (ARGs) and antibiotic-resistant bacteria (ARB) in urban, agricultural, clinical settings, as well extreme environments, have become increasingly relevant One Health perspective. Since Antarctic Arctic regions are considered amongst few remaining pristine environments on Earth, characterization their native resistome appears be utmost importance understand whether how it is evolving result anthropogenic activities climate change. In present review, we report phenotypic (e.g., disk diffusion test) genotypic PCR, metagenomics) approaches used study AMR aquatic environment polar regions, water represents one main dissemination routes nature. Their advantages limits described, emerging trends resulting from analysis ARB ARGs waters discussed. detected these mostly comparable those more anthropized areas, with predominance tetracycline, β-lactam, sulfonamide (and related ARGs). Indeed, is, all cases, consistently highlighted sites impacted by human wildlife respect ones. Surprisingly, aminoglycoside fluroquinolone determinants seem an even higher incidence compared that other areas world, corroborating need for thorough surveillance regions.

Язык: Английский

Процитировано

0

Metagenomics as a Transformative Tool for Antibiotic Resistance Surveillance: Highlighting the Impact of Mobile Genetic Elements with a Focus on the Complex Role of Phages DOI Creative Commons
Nikoline S. Olsen, Leise Riber

Antibiotics, Год журнала: 2025, Номер 14(3), С. 296 - 296

Опубликована: Март 12, 2025

Extensive use of antibiotics in human healthcare as well agricultural and environmental settings has led to the emergence spread antibiotic-resistant bacteria, rendering many infections increasingly difficult treat. Coupled with limited development new antibiotics, rise antimicrobial resistance (AMR) caused a major health crisis worldwide, which calls for immediate action. Strengthening AMR surveillance systems is, therefore, crucial global national efforts combating this escalating threat. This review explores potential metagenomics, sequenced-based approach analyze entire microbial communities without need cultivation, transformative rapid tool improving strategies compared traditional cultivation-based methods. We emphasize importance monitoring mobile genetic elements (MGEs), such integrons, transposons, plasmids, bacteriophages (phages), relation their critical role facilitating dissemination determinants via horizontal gene transfer (HGT) across diverse environments clinical settings. In context, strengths limitations current bioinformatic tools designed detect AMR-associated MGEs metagenomic datasets, including emerging predictive machine learning models, are evaluated. Moreover, controversial phages transmission is discussed alongside phage therapy promising alternative conventional antibiotic treatment.

Язык: Английский

Процитировано

0

Artificial intelligence and its application in clinical microbiology DOI
Assia Mairi,

Lamia Hamza,

Abdelaziz Touati

и другие.

Expert Review of Anti-infective Therapy, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Traditional microbiological diagnostics face challenges in pathogen identification speed and antimicrobial resistance (AMR) evaluation. Artificial intelligence (AI) offers transformative solutions, necessitating a comprehensive review of its applications, advancements, integration clinical microbiology. This examines AI-driven methodologies, including machine learning (ML), deep (DL), convolutional neural networks (CNNs), for enhancing detection, AMR prediction, diagnostic imaging. Applications virology (e.g. COVID-19 RT-PCR optimization), parasitology malaria detection), bacteriology automated colony counting) are analyzed. A literature search was conducted using PubMed, Scopus, Web Science (2018-2024), prioritizing peer-reviewed studies on AI's accuracy, workflow efficiency, validation. AI significantly improves precision operational efficiency but requires robust validation to address data heterogeneity, model interpretability, ethical concerns. Future success hinges interdisciplinary collaboration develop standardized, equitable tools tailored global healthcare settings. Advancing explainable federated frameworks will be critical bridging current implementation gaps maximizing potential combating infectious diseases.

Язык: Английский

Процитировано

0

Perspective Chapter: Molecular Diagnostics in Viral Outbreak Surveillance DOI
Jennifer Giandhari, Amsha Viraragavan, Michelle Gordon

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Understanding and adopting various methods for monitoring viral outbreaks is required pathogen surveillance. Accurate diagnosis can play a significant role in the safe effective treatment prescribed. Africa remains burdened with host of infectious diseases, which challenges healthcare systems proper management diseases. Surveillance are implemented some parts that have infrastructure funding to perform routine testing screening. However, not all this continent equipped necessary tools support. With travel, tourism goods exchange, diseases spread across borders rapidly, posing threat global health, emphasising unified need efforts prevent, detect, act on disease through improved access vaccinations treatments. Effective response depends several elements, including laboratory capacity, skilled health staff, surveillance detect limit illnesses rapidly. Traditional molecular such as genotyping polymerase chain reaction (PCR)-based detection now being complemented like next-generation sequencing clustered regularly interspaced short palindromic repeats (CRISPR). This book chapter aims summarise current broadly describes issue climate change its impact outbreaks.

Язык: Английский

Процитировано

0