Revista Eletrônica Multidisciplinar de Investigação Científica, Год журнала: 2023, Номер 2(5), С. 1 - 11
Опубликована: Июль 7, 2023
valia para entendimento dos casos, uma vez que RNA de vírus pode ser encontrado em amostras esgoto sanitário.A metodologia com análise artigos sites e repositórios também traz grande relevância cientifica se torna algo a explorado cada mais no ambiente pesquisa.
The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953
Опубликована: Март 21, 2023
Язык: Английский
Процитировано
65
The Science of The Total Environment, Год журнала: 2024, Номер 924, С. 171566 - 171566
Опубликована: Март 9, 2024
Wastewater surveillance is a valuable tool that can be used to track infectious diseases in community. In September 2020, the Centers for Disease Control and Prevention (CDC) established National Surveillance System (NWSS) coordinate build nation's capacity detect quantify concentrations of SARS-CoV-2 RNA U.S. wastewater. This first summary NWSS, covering 1, 2020 December 31, 2022. Through partnerships with state, tribal, local, territorial health departments, NWSS became national platform readily expanded adapted meet changing public needs. Beginning 209 sampling sites rapidly >1500 by 2022, ≈47 % population. As >152,000 unique wastewater samples have been collected partners, primarily from treatment plants (WWTPs). WWTPs participating tend larger than average WWTP serve more populated communities. ≈8 nearly 16,000 were NWSS. partners variety methods testing samples; however, progress being made standardize these methods. July 2021, started submitting genome sequencing data October monkeypox virus testing, plans include additional disease targets future. rapid implementation expansion important lessons learned. programs should consider both surge long-term capacities when developing an plan, early standardization facilitate comparisons across sites. has proven flexible sustainable system will continue useful complement case-based guiding action.
Язык: Английский
Процитировано
25
The Science of The Total Environment, Год журнала: 2025, Номер 970, С. 178939 - 178939
Опубликована: Март 1, 2025
Multiplex digital PCR (dPCR) approaches are commonly employed in wastewater-based epidemiology (WBE) studies. However, optimizing the dPCR workflow is a critical step to ensure its reliability and accuracy before application. In this study, 6-plex Crystal Digital PCR® (cdPCR) was optimized for simultaneous detection of six epidemiologically important pathogens, including three enteric viruses, noroviruses genogroups I II (NoV-GI GII) enteroviruses (EnV), respiratory severe acute syndrome coronavirus 2 (SARS-CoV-2), which causative agent disease 2019 (COVID-19), influenza A virus (InfA), syncytial B (RSVB), wastewater. Four cDNA input ratios (20 %-70 %) two extraction kits were evaluated optimization, with 30 % AllPrep PowerViral DNA/RNA Kit (Qiagen) exhibiting optimal performance. The cdPCR assay applied year-long wastewater surveillance study Japan (n = 52), revealing distinct trends prevalence viruses. NoV-GII detected 96 samples highest mean concentration (6.1 ± 0.6 log10 copies/L), while SARS-CoV-2 InfA 60 50 samples, respectively, reflected circulation these pathogens within community. Notably, RSVB less frequently (25 %), line fewer cases reported during period. concentrations EnV showed significant positive correlations hand foot mouth herpangina cases, respectively. no observed RSV COVID-19, possibly due testing RSVA more prevalent also cluster outbreaks. These findings demonstrated utility detecting provided insights into community trends, representing an advancement WBE.
Язык: Английский
Процитировано
1
The Science of The Total Environment, Год журнала: 2023, Номер 882, С. 163487 - 163487
Опубликована: Апрель 15, 2023
A rapid virus concentration method is needed to get high throughput. Reliable results of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) detection in wastewater are necessary for applications wastewater-based epidemiology. In this study, an automated filtration using a concentrating pipette (CP Select; Innovaprep) was applied detect SARS-CoV-2 samples with several modifications increase its sensitivity and The performance the CP Select compared other methods (polyethylene glycol precipitation direct capture silica column) evaluate applicability wastewater. RNA successfully detected six eight method, whereas could all samples. Enteric viruses, such as noroviruses genogroups I (NoVs-GI) II (NoVs-GII) enteroviruses, were tested, resulting 100 % NoVs-GII methods. As NoVs-GI gave comparable number This study showed that optimized less sensitive than methods, applicable or recover viruses
Язык: Английский
Процитировано
15
The Science of The Total Environment, Год журнала: 2024, Номер 945, С. 174140 - 174140
Опубликована: Июнь 19, 2024
Язык: Английский
Процитировано
5
The Science of The Total Environment, Год журнала: 2023, Номер 900, С. 166136 - 166136
Опубликована: Авг. 10, 2023
Язык: Английский
Процитировано
10
Microorganisms, Год журнала: 2024, Номер 12(7), С. 1430 - 1430
Опубликована: Июль 14, 2024
Wastewater monitoring provides essential information about water quality and the degree of contamination. Monitoring these waters helps identify manage risks to public health, prevent spread disease, protect environment. Standardizing appropriate most accurate methods for isolation identification viruses in wastewater is necessary. This review aims present major classes wastewater, as well concentration, isolation, assess health implement corrective measures control viral infections. Last but not least, we propose evaluate current strategies treatment new alternative disinfection.
Язык: Английский
Процитировано
4
Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(48), С. 106660 - 106670
Опубликована: Сен. 21, 2023
Crucial information on the pandemic's spread has been gathered by monitoring trend of SARS-CoV-2 in wastewater. This surveillance highlighted that initial concentration is a critical step analytical procedure due to low viral titer may be present this matrix. paper presents results evaluation two different wastewater protocols determine most efficient and cost-effective. The methods tested were following: (a) biphasic separation system with PEG-dextran (b) PEG/NaCl precipitation protocol. Other aspects detection method also investigated including influence storage temperature virus recovery heat treatment pasteurization, which aims make samples safer for operators environment. was found perform better than system, allowing more sensitive identification presence higher identified all results. Storage at 4.3±0.2°C up 3 weeks did not adversely affect pasteurization pre-treatment increases operator safety maintains concentration.
Язык: Английский
Процитировано
9
Journal of Virological Methods, Год журнала: 2024, Номер 327, С. 114918 - 114918
Опубликована: Март 29, 2024
Язык: Английский
Процитировано
3
Human Genomics, Год журнала: 2024, Номер 18(1)
Опубликована: Май 20, 2024
Abstract Background After the occurrence of COVID-19 pandemic, detection other disseminated respiratory viruses using highly sensitive molecular methods was declared essential for monitoring spread health-threatening in communities. The development multiplex assays are simultaneous such even at low concentrations. In present study, a and specific one-step droplet digital PCR (RT-ddPCR) assay developed absolute quantification influenza A (IAV), B (IBV), syncytial virus (RSV), beta-2-microglobulin transcript as an endogenous internal control (IC B2M). Results first evaluated analytical sensitivity specificity, linearity, reproducibility, recovery rates with excellent performance characteristics then applied to 37 wastewater samples previously commercially available in-house quantitative real-time reverse transcription (RT-qPCR) assays. IAV detected 16/37 (43%), IBV 19/37 (51%), RSV 10/37 (27%) samples. Direct comparison RT-qPCR showed statistically significant high agreement (kappa Cohen’s correlation coefficient: 0.834, p = 0.001) (kappa: 0.773, between two assays, while results 0.355, 0.27) good without statistical significance. Conclusions Overall, ddPCR is cost-effective, specific, can simultaneously detect three common complex matrix Due its resistance inhibitors, could be further used early warning system monitoring.
Язык: Английский
Процитировано
3