Tango-seq: overlaying transcriptomics on connectomics to identify neurons downstream of Drosophila clock neurons DOI Creative Commons

Alison Ehrlich,

Audrey Xu,

Sofia Luminari

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 22, 2024

Summary Knowing how neural circuits change with neuronal plasticity and differ between individuals is important to fully understand behavior. Connectomes are typically assembled using electron microscopy, but this low throughput impractical for analyzing or mutations. Here, we modified the trans -Tango genetic circuit-tracing technique identify neurons synaptically downstream of Drosophila s-LNv clock neurons, which show 24hr rhythms. target were labeled specifically in adult flies a nuclear reporter gene, facilitated their purification then single cell sequencing. We call Tango-seq, it allows transcriptomic data – thus identity be overlayed on top anatomical data. found that s-LNvs preferentially make synaptic connections subset CNMa+ DN1p these likely plastic connections. also identified mushroom body Kenyon cells. Tango-seq should useful addition connectomics toolkit.

Язык: Английский

Memory and the circadian system: Identifying candidate mechanisms by which local clocks in the brain may regulate synaptic plasticity DOI Creative Commons
Matthew J. Hartsock, Robert L. Spencer

Neuroscience & Biobehavioral Reviews, Год журнала: 2020, Номер 118, С. 134 - 162

Опубликована: Июль 23, 2020

Язык: Английский

Процитировано

35
Integration of Circadian Clock Information in the Drosophila Circadian Neuronal Network DOI Creative Commons
Myra Ahmad, Wanhe Li, Deniz Top

и другие.

Journal of Biological Rhythms, Год журнала: 2021, Номер 36(3), С. 203 - 220

Опубликована: Март 1, 2021

Circadian clocks are biochemical time-keeping machines that synchronize animal behavior and physiology with planetary rhythms. In Drosophila, the core components of clock comprise a transcription/translation feedback loop expressed in seven neuronal clusters brain. Although it is increasingly evident each regulated differently, how these communicate other across circadian network less clear. Here, we review latest evidence describes physical connectivity . Using small ventral lateral neurons as starting point, summarize one may another, highlighting signaling pathways both upstream downstream clocks. We propose additional efforts required to understand temporal information generated neuron integrated circuit regulate rhythmic behavior.

Язык: Английский

Процитировано

30
Neurofibromin 1 in mushroom body neurons mediates circadian wake drive through activating cAMP–PKA signaling DOI Creative Commons
Pedro Machado Almeida,

Blanca Lago Solis,

Luca Stickley

и другие.

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Окт. 1, 2021

Abstract Various behavioral and cognitive states exhibit circadian variations in animals across phyla including Drosophila melanogaster , which only ~0.1% of the brain’s neurons contain clocks. Clock transmit timing information to a plethora non-clock via poorly understood mechanisms. Here, we address molecular underpinning this phenomenon by profiling gene expression that constitute mushroom body, center associative learning sleep regulation. We show clocks drive rhythmic hundreds genes body neurons, Neurofibromin 1 ( Nf1 ) tumor suppressor Pka-C1 . Circadian also calcium rhythms NF1-cAMP/PKA-C1 signaling, eliciting higher activity during day than at night, thereby promoting daytime wakefulness. These findings reveal pervasive, non-cell-autonomous regulation brain its role sleep.

Язык: Английский

Процитировано

29
Circadian rhythms as modulators of brain health during development and throughout aging DOI Creative Commons
Rachel Van Drunen, Kristin Eckel‐Mahan

Frontiers in Neural Circuits, Год журнала: 2023, Номер 16

Опубликована: Янв. 19, 2023

The circadian clock plays a prominent role in neurons during development and throughout aging. This review covers topics pertinent to the of 24-h rhythms neuronal function, their tendency decline with Pharmacological or behavioral modification that augment function our internal may be central cognitive disease future chronotherapy for aging-related diseases nervous system.

Язык: Английский

Процитировано

12
Tango-seq: overlaying transcriptomics on connectomics to identify neurons downstream of Drosophila clock neurons DOI Creative Commons

Alison Ehrlich,

Audrey Xu,

Sofia Luminari

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 22, 2024

Summary Knowing how neural circuits change with neuronal plasticity and differ between individuals is important to fully understand behavior. Connectomes are typically assembled using electron microscopy, but this low throughput impractical for analyzing or mutations. Here, we modified the trans -Tango genetic circuit-tracing technique identify neurons synaptically downstream of Drosophila s-LNv clock neurons, which show 24hr rhythms. target were labeled specifically in adult flies a nuclear reporter gene, facilitated their purification then single cell sequencing. We call Tango-seq, it allows transcriptomic data – thus identity be overlayed on top anatomical data. found that s-LNvs preferentially make synaptic connections subset CNMa+ DN1p these likely plastic connections. also identified mushroom body Kenyon cells. Tango-seq should useful addition connectomics toolkit.

Язык: Английский

Процитировано

4