Distributed coding of choice, action and engagement across the mouse brain

Nature, Год журнала: 2019, Номер 576(7786), С. 266 - 273

Опубликована: Ноя. 27, 2019

Язык: Английский

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Natural behavior is the language of the brain

Current Biology, Год журнала: 2022, Номер 32(10), С. R482 - R493

Опубликована: Май 1, 2022

Язык: Английский

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Why is everyone talking about brain state?

Trends in Neurosciences, Год журнала: 2023, Номер 46(7), С. 508 - 524

Опубликована: Май 8, 2023

The rapid and coordinated propagation of neural activity across the brain provides foundation for complex behavior cognition. Technical advances neuroscience subfields have advanced understanding these dynamics, but points convergence are often obscured by semantic differences, creating silos subfield-specific findings. In this review we describe how a parsimonious conceptualization state as fundamental building block whole-brain offers common framework to relate findings scales species. We present examples diverse techniques commonly used study states associated with physiology higher-order cognitive processes, discuss integration them will enable more comprehensive mechanistic characterization dynamics that crucial survival disrupted in disease.

Язык: Английский

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The Cortical States of Wakefulness

Frontiers in Systems Neuroscience, Год журнала: 2019, Номер 12

Опубликована: Янв. 8, 2019

Cortical neurons process information on a background of spontaneous, ongoing activity with distinct spatiotemporal profiles defining different cortical states. During wakefulness, states alter constantly in relation to behavioral context, attentional level or general motor activity. In this review, we will discuss our current understanding awake rodents, how they are controlled, their impact sensory processing, and highlight areas for future research. A common observation rodents is the rapid change spontaneous from high-amplitude, low-frequency fluctuations, when animals quiet, faster smaller fluctuations active. This transition typically thought as global brain state but recent work has shown variation across regions, indicating presence fine spatial scale control system. areas, mediated by at least two convergent inputs, one thalamus other cholinergic inputs basal forebrain. have major balance between specific subtypes neurons, synchronization nearby well functional coupling distant areas. reorganization networks strongly affects processing. Thus provide dynamic system moment-by-moment regulation

Язык: Английский

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Task-evoked activity quenches neural correlations and variability across cortical areas

PLoS Computational Biology, Год журнала: 2020, Номер 16(8), С. e1007983 - e1007983

Опубликована: Авг. 3, 2020

Many large-scale functional connectivity studies have emphasized the importance of communication through increased inter-region correlations during task states. In contrast, local circuit demonstrated that states primarily reduce among pairs neurons, likely enhancing their information coding by suppressing shared spontaneous activity. Here we sought to adjudicate between these conflicting perspectives, assessing whether co-active brain regions tend increase or decrease correlations. We found variability and across a variety cortical in two highly distinct data sets: non-human primate spiking human magnetic resonance imaging data. Moreover, this observed correlation reduction was accompanied an overall dimensionality (reflecting less redundancy) states, suggesting decreased capacity. further both neural mass computational models task-evoked activity stability around stable attractor, globally quenching Together, our results provide integrative mechanistic account encompasses measures activity, variability, resting

Язык: Английский

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Sensory coding and the causal impact of mouse cortex in a visual decision

eLife, Год журнала: 2021, Номер 10

Опубликована: Июль 30, 2021

Correlates of sensory stimuli and motor actions are found in multiple cortical areas, but such correlates do not indicate whether these areas causally relevant to task performance. We trained mice discriminate visual contrast report their decision by steering a wheel. Widefield calcium imaging Neuropixels recordings cortex revealed stimulus-related activity (VIS) frontal (MOs) widespread movement-related across the whole dorsal cortex. Optogenetic inactivation biased choices only when targeted at VIS MOs,proportionally each site's encoding stimulus, times corresponding peak stimulus decoding. A neurometric model based on summing subtracting MOs successfully described behavioral performance predicted effect optogenetic inactivation. Thus, signals localized play causal role performance, while correlating with movement reflect processes that role.

Язык: Английский

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Localized semi-nonnegative matrix factorization (LocaNMF) of widefield calcium imaging data

PLoS Computational Biology, Год журнала: 2020, Номер 16(4), С. e1007791 - e1007791

Опубликована: Апрель 13, 2020

Widefield calcium imaging enables recording of large-scale neural activity across the mouse dorsal cortex. In order to examine relationship these signals resulting behavior, it is critical demix recordings into meaningful spatial and temporal components that can be mapped onto well-defined brain regions. However, no current tools satisfactorily extract different regions in individual mice a data-driven manner, while taking account mouse-specific preparation-specific differences. Here, we introduce Localized semi-Nonnegative Matrix Factorization (LocaNMF), method efficiently decomposes widefield video data allows us directly compare multiple by outputting localized functional are significantly more interpretable than traditional decomposition techniques. Moreover, provides natural subspace correlation maps dynamics behaviors, mice, experimental conditions, identification task- movement-related

Язык: Английский

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Wide-Field Calcium Imaging of Dynamic Cortical Networks during Locomotion

Cerebral Cortex, Год журнала: 2021, Номер 32(12), С. 2668 - 2687

Опубликована: Сен. 15, 2021

Motor behavior results in complex exchanges of motor and sensory information across cortical regions. Therefore, fully understanding the cerebral cortex's role requires a mesoscopic-level description regions engaged, their functional interactions, how these interactions change with behavioral state. Mesoscopic Ca2+ imaging through transparent polymer skulls mice reveals elevated activation dorsal cortex during locomotion. Using correlations between time series fluorescence from 28 (nodes) obtained using spatial independent component analysis (sICA), we examined changes connectivity rest to locomotion goal state that facilitate Both transitions show marked increases correlation among most nodes. However, once steady continued is reached, many nodes, including primary somatosensory decreases correlations, while retrosplenial anterior nodes secondary increases. These highlight cortex, representing on return rest.

Язык: Английский

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A deep-learning approach for online cell identification and trace extraction in functional two-photon calcium imaging

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Март 22, 2022

Abstract In vivo two-photon calcium imaging is a powerful approach in neuroscience. However, processing data computationally intensive and time-consuming, making online frame-by-frame analysis challenging. This especially true for large field-of-view (FOV) imaging. Here, we present CITE-On (Cell Identification Trace Extraction Online), convolutional neural network-based algorithm fast automatic cell identification, segmentation, identity tracking, trace extraction data. processes thousands of cells online, including during mesoscopic imaging, extracts functional measurements from most neurons the FOV. Applied to publicly available datasets, offline version achieves performance similar that state-of-the-art methods analysis. Moreover, generalizes across indicators, brain regions, acquisition parameters anesthetized awake head-fixed mice. represents tool speed up image facilitate closed-loop approaches, example combined all-optical manipulation experiments.

Язык: Английский

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Multiregion neuronal activity: the forest and the trees

Nature reviews. Neuroscience, Год журнала: 2022, Номер 23(11), С. 683 - 704

Опубликована: Окт. 3, 2022

Язык: Английский

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