Multisensory integration enhances audiovisual responses in the Mauthner cell

eLife, Год журнала: 2024, Номер 13

Опубликована: Июль 26, 2024

Multisensory integration (MSI) combines information from multiple sensory modalities to create a coherent perception of the world. In contexts where is limited or equivocal, it also allows animals integrate individually ambiguous stimuli into clearer more accurate percept and, thus, react with adaptive behavioral response. Although responses multisensory have been described at neuronal and levels, causal direct link between these two still missing. this study, we studied audiovisual inputs in Mauthner cell, command neuron necessary sufficient trigger stereotypical escape response fish. We performed intracellular recordings adult goldfish while presenting diverse range determine which stimulus properties affect their integration. Our results show that modality, intensity, temporal structure, interstimulus delay input summation. Mechanistically, found distinct decay dynamics FFI triggered by auditory visual can account for certain aspects Altogether, rare example characterization MSI cell clear relevance, providing both phenomenological mechanistic insights how depends on properties.

Язык: Английский

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Brain-wide circuitry underlying altered auditory habituation in zebrafish models of autism.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 5, 2024

Auditory processing is widely understood to occur differently in autism, though the patterns of brain activity underlying these differences are not well understood. The diversity autism also means brain-wide networks may change various ways produce similar behavioral outputs. We used larval zebrafish investigate auditory habituation four genetic lines relevant autism:

Язык: Английский

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NemoTrainer: Automated Conditioning for Stimulus-Directed Navigation and Decision Making in Free-Swimming Zebrafish

Animals, Год журнала: 2022, Номер 13(1), С. 116 - 116

Опубликована: Дек. 28, 2022

Current methods for associative conditioning in animals involve human intervention that is labor intensive, stressful to animals, and introduces experimenter bias the data. Here, we describe a simple apparatus flexible, microcontroller-based paradigm minimizes intervention. Our methodology exploits directed movement towards target depends on spatial working memory, including processing of sensory inputs, motivational drive, attentional mechanisms. Within stimulus-driven designed train zebrafish, present localized pulse light via LEDs and/or sounds an underwater transducer. A webcam placed below glass tank records fish-swimming behavior. For classical conditioning, simply associate sound or with unconditioned stimulus, such as small food reward presented at fixed location, swim location obtain few grains dispensed automatically sensor-triggered, stepper motor. During operant fish must first approach proximity sensor remote then location. both types timing-gated interrupt activates motors custom software embedded within microcontroller (Arduino). "Ardulink", Java facility, implements Arduino-computer communication protocols. In this way, Java-based user interface running host computer can provide full experimental control. Alternatively, similar level control achieved Arduino script communicating event-driven application controller computer. Either enable precise, multi-day scheduling training, timing, intensity stimulus parameters; feeder. Learning be tracked by monitoring turning, response times, directional swimming individual fish. This facilitates comparison performance across cohort animals. ("NemoTrainer") used study multiple aspects species-specific behaviors well effects them various interventions.

Язык: Английский

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Brain-wide impacts of sedation on spontaneous activity and auditory processing in larval zebrafish

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 30, 2024

Abstract Despite their widespread use, we have limited knowledge of the mechanisms by which sedatives mediate effects on brain-wide networks. This is, in part, due to technical challenge observing activity across large populations neurons normal and sedated brains. In this study, examined sedative dexmedetomidine, its antagonist atipamezole, spontaneous brain dynamics auditory processing zebrafish larvae. Our brain-wide, cellular-resolution calcium imaging reveals, for first time, regions involved these network-scale individual that are affected within those regions. Further analysis reveals a variety dynamic changes at baseline, including marked reductions activity, correlation, variance. The variance represent “quieter” state during sedation, an effect causes highly correlated evoked system stand out more than it does un-sedated We also observe reduction response latencies suggesting removal leaves core pathway free impingement from other non-auditory information. Finally, describe less network with higher energy barrier lower probability transitions sedation. total, our shows sedation leads quieter, stable, brain, against background, responses become sharper prominent. Significance Statement Animals’ states constantly fluctuate environment context, leading perception behavioral choices. Alterations perception, sensorimotor gating, selection hallmarks numerous neuropsychiatric disorders, but circuit- network-level underpinnings alterations poorly understood. Pharmacological alters responsiveness provides controlled repeatable manipulation studying underlying circuitry. Here, show larval dexmedetomidine reduces locomotion portions behavior intact. computationally model levels neurons, local circuits, networks lead altered sensory

Язык: Английский

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Multisensory integration enhances audiovisual responses in the Mauthner cell

Опубликована: Ноя. 25, 2024

Multisensory integration combines information from multiple sensory modalities to create a coherent perception of the world. In contexts where is limited or equivocal, it also allows animals integrate individually ambiguous stimuli into clearer more accurate percept and, thus, react with adaptive behavioral response. Although responses multisensory have been described at neuronal and levels, causal direct link between these two still missing. this study, we studied audiovisual inputs in Mauthner cell, command neuron necessary sufficient trigger stereotypical escape response fish. We performed intracellular recordings adult goldfish while presenting diverse range determine which stimulus properties affect their integration. Our results show that modality, intensity, temporal structure, interstimulus delay input summation. Mechanistically, found distinct decay dynamics feedforward inhibition triggered by auditory visual can account for certain aspects Altogether, rare example characterization cell clear relevance, providing both phenomenological mechanistic insights how depends on properties.

Язык: Английский

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