A microRNA that controls the emergence of embryonic movement DOI Creative Commons
Jonathan A. C. Menzies, André Maia Chagas, Tom Baden

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Июнь 13, 2024

Movement is a key feature of animal systems, yet its embryonic origins are not fully understood. Here, we investigate the genetic basis underlying onset movement in Drosophila focusing on role played by small non-coding RNAs (microRNAs, miRNAs). To this end, first develop quantitative behavioural pipeline capable tracking large populations fly embryos, and using system, discover that miRNA miR-2b-1 plays emergence movement. Through combination spectral analysis motor patterns, cell sorting RNA situs, reconstitution tests, neural optical imaging define influences exerting actions developing nervous system. Furthermore, through bioinformatics coupled to manipulation expression phenocopy tests identify previously uncharacterised (but evolutionarily conserved) chloride channel encoding gene – which term Mo vement Modula tor ( Motor ) as target mechanistically links Cell-specific null mutant background, followed assays analyses, suggest affects effects sensory elements circuitry, rather than domain. Our work thus reports system regulating movement, suggesting other miRNAs likely play developmental process well species.

Язык: Английский

The gut contractile organoid: a novel model for studying the gut motility regulated by coordinating signals between interstitial cells of Cajal and smooth muscles DOI Open Access

Rei Yagasaki,

Ryô Nakamura, Yuuki Shikaya

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 13, 2024

The gut undergoes peristaltic movements regulated by intricate cellular interactions. How there interactions emerge in the developing remains poorly explored due to a lack of model system.We here developed novel contractile organoid that is derived from muscle layer chicken embryonic hindgut. contained smooth cells (SMCs) and interstitial Cajal (ICCs; pacemaker) with few enteric neurons, underwent periodic contractions. formed self-organization morphological arrangements ICCs (internal) SMCs (peripheral), allowing identification these live. GCaMP-Ca2+ imaging analyses revealed Ca2+ transients between ICC-ICC, SMC-SMC or SMC-ICC were markedly coordinated. Pharmacological studies further suggested role gap junctions ICC-to-SMC signaling, also possible mechanical feedback SMC's contraction ICC's pace-making activities. In addition, two organoids different rhythm became synchronized when mediated SMCs, unveiling contribution pace-making. this study offers useful understand how coordination between/among maintained during development.

Язык: Английский

Процитировано

0
A microRNA that controls the emergence of embryonic movement DOI Open Access
Jonathan A. C. Menzies, André Maia Chagas, Claudio R. Alonso

и другие.

Опубликована: Март 20, 2024

Movement is a key feature of animal systems, yet its embryonic origins are not fully understood. Here we investigate the genetic basis underlying onset movement in Drosophila focusing on role played by small non-coding RNAs (microRNAs, miRNAs). To this end, first develop quantitative behavioural pipeline capable tracking large populations fly embryos, and using system, discover that miRNA miR-2b-1 plays emergence movement. Through combination spectral analysis motor patterns, cell sorting RNA situs , reconstitution tests, neural optical imaging define influences exerting actions developing nervous system. Furthermore, through bioinformatics coupled to manipulation expression phenocopy tests identify previously uncharacterised (but evolutionarily conserved) chloride channel encoding gene – which term Janus as target mechanistically links Cell-specific null mutant background, followed assays analyses, suggest affects effects sensory elements circuitry, rather than domain. Our work thus reports system regulating movement, suggesting other miRNAs likely play developmental process well species.

Язык: Английский

Процитировано

0
CRASH2p: Closed-loop Two Photon Imaging in Freely Moving Animals DOI Creative Commons

P. J. McNulty,

Rui Wu, Akihiro Yamaguchi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 26, 2024

Direct measurement of neural activity in freely moving animals is essential for understanding how the brain controls and represents behaviors. Genetically encoded calcium indicators report as changes fluorescence intensity, but motion confounds quantitative fluorescence. Translation, rotation, deformation movements intervening scattering or auto-fluorescent tissue all alter amount fluorescent light captured by a microscope. Compared to single-photon approaches, two photon microscopy less sensitive off-target fluorescence, more motion, imaging has always required anchoring microscope brain. We developed closed-loop resonant axial-scanning high-speed (CRASH2p) real-time 3D correction unrestrained animals, without implantation reference markers. complemented CRASH2p with novel scanning strategy multi-stage registration pipeline. performed volumetric ratiometrically corrected functional CNS Drosophila larvae discovered previously unknown correlates behavior.

Язык: Английский

Процитировано

0
A microRNA that controls the emergence of embryonic movement DOI Open Access
Jonathan A. C. Menzies, André Maia Chagas, Tom Baden

и другие.

Опубликована: Июнь 4, 2024

Movement is a key feature of animal systems, yet its embryonic origins are not fully understood. Here we investigate the genetic basis underlying onset movement in Drosophila focusing on role played by small non-coding RNAs (microRNAs, miRNAs). To this end, first develop quantitative behavioural pipeline capable tracking large populations fly embryos, and using system, discover that miRNA miR-2b-1 plays emergence movement. Through combination spectral analysis motor patterns, cell sorting RNA situs , reconstitution tests, neural optical imaging define influences exerting actions developing nervous system. Furthermore, through bioinformatics coupled to manipulation expression phenocopy tests identify previously uncharacterised (but evolutionarily conserved) chloride channel encoding gene – which term Mo vement Modula tor ( Motor) as target mechanistically links Cell-specific null mutant background, followed assays analyses, suggest affects effects sensory elements circuitry, rather than domain. Our work thus reports system regulating movement, suggesting other miRNAs likely play developmental process well species.

Язык: Английский

Процитировано

0
A microRNA that controls the emergence of embryonic movement DOI Creative Commons
Jonathan A. C. Menzies, André Maia Chagas, Tom Baden

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Июнь 13, 2024

Movement is a key feature of animal systems, yet its embryonic origins are not fully understood. Here, we investigate the genetic basis underlying onset movement in Drosophila focusing on role played by small non-coding RNAs (microRNAs, miRNAs). To this end, first develop quantitative behavioural pipeline capable tracking large populations fly embryos, and using system, discover that miRNA miR-2b-1 plays emergence movement. Through combination spectral analysis motor patterns, cell sorting RNA situs, reconstitution tests, neural optical imaging define influences exerting actions developing nervous system. Furthermore, through bioinformatics coupled to manipulation expression phenocopy tests identify previously uncharacterised (but evolutionarily conserved) chloride channel encoding gene – which term Mo vement Modula tor ( Motor ) as target mechanistically links Cell-specific null mutant background, followed assays analyses, suggest affects effects sensory elements circuitry, rather than domain. Our work thus reports system regulating movement, suggesting other miRNAs likely play developmental process well species.

Язык: Английский

Процитировано

0