Taste triggers a homeostatic temperature control in Drosophila

Опубликована: Фев. 28, 2024

Hungry animals consistently show a desperate desire to obtain food. Even brief sensory detection of food can trigger bursts physiological and behavioral changes. However, the underlying mechanisms by which sensation triggers acute response remain elusive. We have previously shown in Drosophila that hunger drives preference for low temperature. Because is small ectotherm, temperature implies body metabolic rate. Here, we taste sensing switch from high hungry flies. stimulation artificial sweeteners or optogenetics an warm preference, but not sufficient reach fed state. Instead, nutrient intake required The data suggest starvation recovery controlled two components: taste-evoked nutrient-induced preferences, quality play distinct roles recovery. Animals are motivated eat based on time day hunger. found clock genes signals profoundly control preferences. Thus, our one critical layers regulatory representing internal energy homeostasis metabolism.

Язык: Английский

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Molecular characterization of gustatory second-order neurons reveals integrative mechanisms of gustatory and metabolic information

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 17, 2024

Abstract Animals must balance the urgent need to find food during starvation with critical necessity avoid toxic substances ensure their survival. In Drosophila , specialized Gustatory Receptors (GRs) expressed in Receptor Neurons (GRNs) are for distinguishing between nutritious and potentially food. GRNs project axons from taste organs Subesophageal Zone (SEZ) Central Brain (CB) of where gustatory information is processed. Although roles GRs well- documented, processing SEZ remains unclear. To better understand sensory feeding decision-making, we molecularly characterized first layer interneurons, referred as Second Order (G2Ns), which receive direct input GRNs. Using trans-synaptic tracing trans- Tango, cell sorting, bulk RNAseq under fed starved conditions, discovered that G2Ns vary based on molecular profile changes fly’s metabolic state. Further data analysis has revealed a pair neurons SEZ, expressing neuropeptide Leucokinin (SELK neurons), simultaneous sensing bitter (potentially toxic) sweet (nutritious) information. Additionally, these also inputs regarding levels fly. These results highlight novel mechanism regulation integration.

Язык: Английский

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Evolution of taste processing shifts dietary preference

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Abstract Food choice is an important driver of speciation and invasion novel ecological niches. However, we know little about the mechanisms leading to changes in dietary preference. Here, use three closely-related species Drosophila sechellia , D. simulans melanogaster study taste circuit food evolution. sechellia, a host specialist, feeds exclusively on single fruit ( Morinda citrifolia noni) - latter two are generalists living various substrates. Using quantitative feeding assays, recapitulate preference for noni detect conserved sweet but altered bitter sensitivity via calcium imaging peripheral neurons. Noni surprisingly activates sensing neurons more strongly due small deletion one gustatory receptor. volumetric ventral brain, show that instead physiology, species-specific processing sugar signals sensorimotor circuits recapitulates differences Our data support receptor alone cannot explain rather modifications how sensory information transformed into motor commands.

Язык: Английский

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Convergent olfactory circuits for courtship inDrosophilarevealed byds-Tango

Опубликована: Окт. 23, 2024

Summary Animals exhibit sex-specific behaviors that are governed by sexually dimorphic circuits. One such behavior in male Drosophila melanogaster , courtship, is regulated various sensory modalities, including olfaction. Here, we reveal how olfactory pathways flies converge at the third-order, onto lateral horn output neurons, to regulate courtship. To achieve this, developed ds -Tango, a modified version of monosynaptic tracing and manipulation tool trans- Tango. In two distinct configurations Tango positioned series, thus providing selective genetic access not only partners starter neurons but also their disynaptic connections. Using identified node convergence for three pathways. Silencing this results deficits sex recognition potential partners. Our identify required proper courtship establish -Tango as circuit tracing.

Язык: Английский

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Molecular characterization of gustatory second-order neurons reveals integrative mechanisms of gustatory and metabolic information

Опубликована: Ноя. 8, 2024

Animals must balance the urgent need to find food during starvation with critical necessity avoid toxic substances ensure their survival. In Drosophila , specialized Gustatory Receptors (GRs) expressed in Receptor Neurons (GRNs) are for distinguishing between nutritious and potentially food. GRNs project axons from taste organs Subesophageal Zone (SEZ) Central Brain (CB) of where gustatory information is processed. Although roles GRs well- documented, processing SEZ remains unclear. To better understand sensory feeding decision-making, we molecularly characterized first layer interneurons, referred as Second Order (G2Ns), which receive direct input GRNs. Using trans-synaptic tracing trans- Tango, cell sorting, bulk RNAseq under fed starved conditions, discovered that G2Ns vary based on molecular profile changes fly’s metabolic state. Further data analysis has revealed a pair neurons SEZ, expressing neuropeptide Leucokinin (SELK neurons), simultaneous sensing bitter (potentially toxic) sweet (nutritious) information. Additionally, these also inputs regarding levels fly. These results highlight novel mechanism regulation integration.

Язык: Английский

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