Editorial: Diversity of beetles and associated microorganisms

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Июль 26, 2023

EDITORIAL article Front. Microbiol., 26 July 2023Sec. Microbial Symbioses Volume 14 - 2023 | https://doi.org/10.3389/fmicb.2023.1252736

Язык: Английский

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Field survey of Cassidinae beetles (Coleoptera, Chrysomelidae) and their host plants in southern Guangxi, China

Biodiversity Data Journal, Год журнала: 2023, Номер 11

Опубликована: Июль 31, 2023

Few systematic studies have been conducted on the faunal composition and food web structure of Cassidinae China. During 2013-2019, we systematically investigated beetles their host plants in southern Guangxi. A total 2,255 individuals from 66 species, 23 genera ten tribes were collected Most species belonged to tribe Hispini (23 34.8%), followed by Gonophorini (13 19.7%), Cassidini (eight 12.1%) Aspidimorphini (six 9.1%). The others (16 species) Anisoderini, Botryonopini, Callispini, Oncocephalini, Notosacanthini Leptispini. was recorded Guangxi for first time. Neownesia (Botryonopini), Gonophora (Gonophorini), Micrispa Notosacantha (Notosacanthini) Prionispa (Oncocephalini) firstly In total, obtained 47 newly-recorded 33 whole Guangxi, which, Callispa frontalis Medvedev, 1992 newly Dactylispa feae Gestro (625 individuals) D. chinensis Weise (565 most common species. 69 53 19 families identified Many plant associations are new records Cassidinae. Quantitative analysis indicated that primarily fed Poaceae, Convolvulaceae, Cyperaceae Rosaceae. Generally, plant-Cassidinae webs moderately complex stable This is large contribution knowledge diversity

Язык: Английский

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Adipokinetic hormone signaling mediates the enhanced fecundity ofDiaphorina citriinfected by ‘CandidatusLiberibacter asiaticus’

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 27, 2023

Abstract Diaphorina citri serves as the primary vector for ‘ Candidatus Liberibacter asiaticus’ ( C Las), bacterium associated with severe Asian form of huanglongbing. Las-positive D. are more fecund than their Las-negative counterparts and require extra energy expenditure. Therefore, understanding molecular mechanisms linking metabolism reproduction is particular importance. In this study, we found adipokinetic hormone DcAKH ) its receptor DcAKHR were essential increasing lipid fecundity in response to Las infection citri. Knockdown not only resulted accumulation triacylglycerol a decline glycogen, but also significantly decreased titer ovaries. Combined vivo vitro experiments showed that miR-34 suppresses expression by binding 3’ untranslated region, whilst overexpression ovaries caused defects mimicked knockdown phenotypes. Additionally, reduced juvenile (JH) JH signaling pathway genes fat bodies ovaries, including receptor, methoprene-tolerant DcMet ), transcription factor, Krüppel homolog 1 (DcKr-h1) , acts downstream it, well egg development related vitellogenin 1-like DcVg-1-like A1-like DcVg-A1-like DcVgR ). As result, hijacks AKH/AKHR-miR-34-JH improve fecundity, while simultaneously replication Las, suggesting mutualistic interaction between

Язык: Английский

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Extracellular symbiont colonizes insect during embryo development

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 5, 2023

Abstract Insects typically acquire their beneficial microbes early in development. Endosymbionts housed intracellularly are commonly integrated during oogenesis or embryogenesis, whereas extracellular only known to be acquired after hatching by immature instars such as larvae nymphs. Here, however, we report on an symbiont that colonises its host embryo Tortoise leaf beetles (Chrysomelidae: Cassidinae) digestive bacterial Stammera extracellularly within foregut symbiotic organs, and ovary-associated glands ensure vertical transmission. We outline the initial stages of colonization observe while organs develop three days prior larval emergence, they remain empty until final 24 hours Infection occurs timeframe, hatching. By experimentally manipulating availability embryos egg, describe a 12-hour developmental window governing . Symbiotic form normally aposymbiotic larvae, demonstrating these -bearing structures autonomously. In adults, already colonized following metamorphosis stable population facilitate folivory. The glands, initially lack Symbiont abundance subsequently increases transmission thereby ensuring sufficient titers at onset oviposition ∼29 metamorphosis. Collectively, our findings reveal precedes where proliferation is eventually decoupled adult match nutritional reproductive requirements host.

Язык: Английский

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Paleocene origin of a streamlined digestive symbiosis in leaf beetles

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 13, 2023

Abstract Timing the acquisition of a beneficial microbe relative to evolutionary history its host can shed light on adaptive impact partnership. Here, we investigated onset and molecular evolution an obligate symbiosis between Cassidinae leaf beetles Candidatus Stammera capleta, γ-proteobacterium. Residing extracellularly within foregut symbiotic organs, upgrades digestive physiology by supplementing plant cell wall-degrading enzymes. We observe that is shared symbiont across tortoise hispine collectively comprise subfamily, despite differences in their folivorous habits. In contrast transcriptional profile during vertical transmission, elevates expression genes encoding enzymes while matching nutritional requirements host. Symbiont Paleocene (∼62 Mya) did not coincide with origin beetles, widespread distribution subfamily. Early-diverging lineages lack specialized organs house it. Reconstructing ancestral state host-beneficial factors revealed encoded three at symbiosis, including polygalacturonase – pectinase universally shared. While non-symbiotic cassidines encode endogenously, repertoire more limited compared supplemented from . Highlighting potential condition upgraded metabolic potential, -harboring exploit greater variety plants are speciose members Cassidinae.

Язык: Английский

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