Journal of Molecular Biology,
Год журнала:
2025,
Номер
unknown, С. 169151 - 169151
Опубликована: Апрель 1, 2025
The
endoplasmic
reticulum
(ER)
is
a
multifunctional
organelle
essential
for
protein
and
lipid
synthesis,
ion
transport
inter-organelle
communication.
It
comprises
highly
dynamic
network
of
membranes
that
continuously
reshape
to
support
wide
range
cellular
processes.
During
differentiation,
extensive
remodelling
both
ER
architecture
its
proteome
required
accommodate
alterations
in
cell
morphology
function.
Autophagy,
ER-phagy
particular,
plays
pivotal
role
reshaping
the
ER,
enabling
cells
meet
their
evolving
needs
adapt
developmental
cues.
Despite
ER's
critical
mechanisms
responsible
regulating
dynamics
are
not
fully
understood.
Emerging
evidence
suggests
transcriptional
post-translational
regulation
play
fine-tuning
unfolded
response
(UPR).
This
review
explores
molecular
basis
autophagy
ER-phagy,
highlighting
during
differentiation.
A
deeper
understanding
these
processes
could
open
new
avenues
targeted
therapeutic
approaches
conditions
where
impaired.
Autophagy,
Год журнала:
2025,
Номер
unknown, С. 1 - 13
Опубликована: Янв. 5, 2025
Macroautophagy
is
a
catabolic
process
that
maintains
cellular
homeostasis
by
recycling
intracellular
material
through
the
use
of
double-membrane
vesicles
called
autophagosomes.
In
turn,
autophagosomes
fuse
with
vacuoles
(in
yeast
and
plants)
or
lysosomes
metazoans),
where
resident
hydrolases
degrade
cargo.
Given
conservation
autophagy,
Porcine
deltacoronavirus
(PDCoV)
is
an
emerging
enteropathogenic
coronavirus
that
causes
severe
diarrhea
in
neonatal
piglets
worldwide
and
presents
a
significant
public
health
threat
due
to
its
potential
for
cross-species
transmission.
Selective
macroautophagy/autophagy,
mediated
by
autophagy
receptors
such
as
NBR1
(NBR1
cargo
receptor),
plays
key
role
restricting
viral
infection
modulating
the
host
immune
response.
In
this
study,
we
revealed
overexpression
of
inhibits
PDCoV
replication,
while
knockdown
increases
titers.
Further
analysis
demonstrated
interacts
with
envelope
(E)
protein
independently
ubiquitination,
directing
it
phagophores
autophagic
degradation
limit
proliferation.
To
counteract
defense,
3C-like
protease,
encoded
NSP5,
cleaves
porcine
at
glutamine
353
(Q353),
impairing
selective
function
antiviral
activity.
Additionally,
NSP5
proteases
from
other
coronaviruses
including
PEDV,
TGEV,
SARS-CoV-2
also
cleave
same
site,
suggesting
employ
conserved
strategy
NSP5-mediated
cleavage
evade
responses
facilitate
infection.
Overall,
our
study
underscores
importance
NBR1-mediated
host's
defense
against
reveals
which
evades
mechanisms
promote
successful
Journal of Molecular Biology,
Год журнала:
2025,
Номер
unknown, С. 169151 - 169151
Опубликована: Апрель 1, 2025
The
endoplasmic
reticulum
(ER)
is
a
multifunctional
organelle
essential
for
protein
and
lipid
synthesis,
ion
transport
inter-organelle
communication.
It
comprises
highly
dynamic
network
of
membranes
that
continuously
reshape
to
support
wide
range
cellular
processes.
During
differentiation,
extensive
remodelling
both
ER
architecture
its
proteome
required
accommodate
alterations
in
cell
morphology
function.
Autophagy,
ER-phagy
particular,
plays
pivotal
role
reshaping
the
ER,
enabling
cells
meet
their
evolving
needs
adapt
developmental
cues.
Despite
ER's
critical
mechanisms
responsible
regulating
dynamics
are
not
fully
understood.
Emerging
evidence
suggests
transcriptional
post-translational
regulation
play
fine-tuning
unfolded
response
(UPR).
This
review
explores
molecular
basis
autophagy
ER-phagy,
highlighting
during
differentiation.
A
deeper
understanding
these
processes
could
open
new
avenues
targeted
therapeutic
approaches
conditions
where
impaired.
