ROS are universal cell-to-cell stress signals

Current Opinion in Plant Biology, Год журнала: 2024, Номер 79, С. 102540 - 102540

Опубликована: Апрель 21, 2024

Язык: Английский

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Rapid systemic responses of Arabidopsis to waterlogging stress

PLANT PHYSIOLOGY, Год журнала: 2023, Номер 193(3), С. 2215 - 2231

Опубликована: Авг. 3, 2023

Waterlogging stress (WLS) negatively impacts the growth and yield of crops resulting in heavy losses to agricultural production. Previous studies have revealed that WLS induces a systemic response shoots is partially dependent on plant hormones ethylene abscisic acid. However, role rapid cell-to-cell signaling pathways, such as reactive oxygen species (ROS) calcium waves, responses plants unknown at present. Here, we reveal an abrupt treatment Arabidopsis (Arabidopsis thaliana) growing peat moss triggers ROS wave WLS-triggered ROS-generating RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD), calcium-permeable channels GLUTAMATE-LIKE RECEPTOR 3.3 3.6 (GLR3.3 GLR3.6), aquaporin PLASMA MEMBRANE INTRINSIC PROTEIN 2;1 (PIP2;1) proteins. We further show accompanied by transcriptomic evident early 10 min following waterlogging initiation, includes many hypoxia-response transcripts, RBOHD. Interestingly, resulted triggering hydraulic transient opening stomata leaves. In addition, it induced heightened state tolerance subsequent submergence stress. Taken together, our findings initiation physiological involve ROS, calcium, well induction hypoxia acclimation mechanisms tissues.

Язык: Английский

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Systemic stomatal responses in plants: Coordinating development, stress, and pathogen defense under a changing climate

Plant Cell & Environment, Год журнала: 2024, Номер 47(4), С. 1171 - 1184

Опубликована: Янв. 1, 2024

Abstract To successfully survive, develop, grow and reproduce, multicellular organisms must coordinate their molecular, physiological, developmental metabolic responses among different cells tissues. This process is mediated by cell‐to‐cell, vascular and/or volatile communication, involves electric, chemical hydraulic signals. Within this context, stomata serve a dual role coordinating to the environment with neighbouring at epidermis, but also other present on parts of plant. As represent one most important conduits between plant its above‐ground environment, as well directly affect photosynthesis, respiration status controlling gas vapour exchange atmosphere, overall response within leaves tissues plays cardinal in growth, development reproduction. Here, we discuss examples local systemic stomatal coordination, signalling pathways that mediate them, importance coordination our food supply, ecosystems weather patterns, under changing climate. We further potential biotechnological implications regulating for enhancing agricultural productivity warmer CO 2 ‐rich environment.

Язык: Английский

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Ca2+/calmodulin-mediated desensitization of glutamate receptors shapes plant systemic wound signalling and anti-herbivore defence

Nature Plants, Год журнала: 2024, Номер 10(1), С. 145 - 160

Опубликована: Янв. 2, 2024

Язык: Английский

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Transformation of flg22 perception into electrical signals decoded in vasculature leads to sieve tube blockage and pathogen resistance

Science Advances, Год журнала: 2025, Номер 11(9)

Опубликована: Фев. 26, 2025

This study focuses on the question how and where information acquired by FLS2 perception of flg22 is transformed into electrical signals crucial for generation local systemic defense responses. In Arabidopsis thaliana Vicia faba leaves, density was high in epidermis vascular parenchyma, low mesophyll, absent sieve elements (SEs). Aequorin-based examinations disclosed dual cytosolic Ca 2+ peaks shortly after application, which corresponded with two voltage shifts from to SEs. These were converted rapid long-range action potentials (APs) or slower short-range variation (VPs). Modified phytohormone-levels demonstrated AP effects. Jasmonic acid up-regulation significantly higher wild-type than Atseor1/2 mutants. Abundant influx associated VPs responsible transient element occlusion (SEO) near site, whereas SEO Atfls2 Biological relevance susceptibility mutants Pseudomonas syringae plants.

Язык: Английский

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Aboveground whole‐plant live imaging method for nitric oxide (NO) reveals an intricate relationship between NO and H₂O₂

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Nitric oxide (NO) is a key regulator of plant development, growth, and responses to the environment. Together with hydrogen peroxide (H2O2), NO modifies structure function proteins, controlling redox signaling. Although has been studied extensively at cellular subcellular levels, very little known about changes in content whole-plant level. Here, we report on development an aboveground live imaging method for NO. Using mutants altered as well donor/scavenger, demonstrate specificity detection Arabidopsis thaliana plants were found produce basal level under control conditions. levels accumulated enzymatically following heat stress applied entire plant, systemic manner different locally stimuli. Similar or opposing accumulation patterns also H2O2 during response Our findings reveal that accumulates local stimulus. In addition, they shed new light intricate relationships between H2O2. The reported opens way multiple future studies NO's role biology.

Язык: Английский

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Rapid Propagation of Ca2+ Waves and Electrical Signals in the Liverwort Marchantia polymorpha

Plant and Cell Physiology, Год журнала: 2024, Номер 65(4), С. 660 - 670

Опубликована: Янв. 9, 2024

Abstract In response to both biotic and abiotic stresses, vascular plants transmit long-distance Ca2+ electrical signals from localized stress sites distant tissues through their vasculature. Various models have been proposed for the mechanisms underlying signaling, primarily centered around presence of bundles. We here demonstrate that non-vascular liverwort Marchantia polymorpha possesses a mechanism propagating waves in wounding. The propagation velocity these was approximately 1–2 mm s-1, equivalent observed plants. Both were inhibited by La3+ as well tetraethylammonium chloride, suggesting crucial importance channel(s) K+ wound-induced membrane depolarization subsequent signal propagation. Simultaneous recordings indicated tight coupling between dynamics two signaling modalities. Furthermore, molecular genetic studies revealed GLUTAMATE RECEPTOR-LIKE (GLR) channel plays central role signals. Conversely, none three two-pore channels implicated either These findings shed light on evolutionary conservation rapid wave involving GLRs land plants, even absence tissue.

Язык: Английский

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Plant electrophysiology with conformable organic electronics: Deciphering the propagation of Venus flytrap action potentials

Science Advances, Год журнала: 2023, Номер 9(30)

Опубликована: Июль 26, 2023

Electrical signals in plants are mediators of long-distance signaling and correlate with plant movements responses to stress. These studied single surface electrodes that cannot resolve signal propagation integration, thus impeding their decoding link function. Here, we developed a conformable multielectrode array based on organic electronics for large-scale high-resolution electrophysiology. We performed precise spatiotemporal mapping the action potential (AP) Venus flytrap found AP actively propagates through tissue constant speed without strong directionality. also spontaneously generated APs can originate from unstimulated hairs they trap movement. Last, demonstrate circuitry be activated by cells other than sensory hairs. Our work reveals key properties establishes capacity bioelectronics resolving electrical contributing mechanistic understanding plants.

Язык: Английский

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Functional analysis of reactive oxygen species‐driven stress systemic signalling, interplay and acclimation

Plant Cell & Environment, Год журнала: 2024, Номер 47(8), С. 2842 - 2851

Опубликована: Март 21, 2024

Abstract Reactive oxygen species (ROS) play a critical role in plant development and stress responses, acting as key components rapid signalling pathways. The ‘ROS wave’ triggers essential acclimation processes, ultimately ensuring survival under diverse challenges. This review explores recent advances understanding the composition functionality of ROS wave within cells. During their initiation propagation, waves interact with other pathways, hormones various molecular compounds. Recent research sheds light on intriguing lack rigid hierarchy governing these interactions, highlighting complex interplay between signals. Notably, culminate systemic acclimation, crucial outcome for enhanced tolerance. emphasizes versatility ROS, which act flexible players network short‐ long‐term factors contributing to resilience. Unveiling intricacies interactions molecules holds immense potential developing strategies augment tolerance, improved agricultural practices overall ecosystem well‐being.

Язык: Английский

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The redox code of plants

Plant Cell & Environment, Год журнала: 2023, Номер 47(8), С. 2821 - 2829

Опубликована: Дек. 13, 2023

Abstract Central metabolism is organised through high‐flux, Nicotinamide Adenine Dinucleotide (NAD + /NADH) and NADP /NADPH systems operating at near equilibrium. As oxygen indispensable for aerobic organisms, these are also linked to the levels of reactive species, such as H 2 O , regulation macromolecular structures activities, via kinetically controlled sulphur switches in redox proteome. Dynamic changes production, scavenging transport, associated with development, growth responses environment are, therefore, state cell regulate cellular function. These basic principles form ‘redox code’ cells were first defined by D. P. Jones H. Sies 2015. Here, we apply plants which recent studies have shown that they can explain cell‐to‐cell even plant‐to‐plant signalling processes. The code is, an integral part biological be used multiple processes subcellular, cellular, tissue, whole organism perhaps community ecosystem levels. environmental conditions on our planet worsening due global warming, climate change increased pollution levels, new needed applying changes.

Язык: Английский

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