The core microbiota across the green lineage DOI Creative Commons
Paloma Durán

Current Opinion in Plant Biology, Год журнала: 2023, Номер 77, С. 102487 - 102487

Опубликована: Дек. 5, 2023

The study of plant–microbe interactions and the characterization plant-associated microbiota has been focus plant researchers in last decades due to its importance for health natural conditions. Here, I explore persistent core associated with different species across environments by performing a meta-analysis publicly available datasets. Intra-specific analyses revealed that diverse genotypes growing similar habitats interact common set microbial groups but some these are species- or environment-specific. Furthermore, interspecific demonstrates conservation seven bacterial orders photosynthetic organisms, including microalgae, suggesting conserved capacity interaction microbes throughout evolutionary history. However, specific functions members whether hosts remain largely unexplored. therefore discuss understanding roles propose future research directions, exploration kingdoms. By investigating functions, it will be possible leverage this knowledge sustainable agricultural management goals.

Язык: Английский

Genetic architecture of heritable leaf microbes DOI Creative Commons
Julia A. Boyle, Megan E. Frederickson, John R. Stinchcombe

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 12(7)

Опубликована: Июнь 6, 2024

Host-associated microbiomes are shaped by both their environment and host genetics, often impact performance. The scale of genetic variation important to microbes is largely unknown yet fundamental the community assembly host-associated microbiomes, with implications for eco-evolutionary dynamics hosts. Using

Язык: Английский

Процитировано

0
Genetic modification of the shikimate pathway to reduce lignin content in switchgrass ( Panicum virgatum L.) significantly impacts plant microbiomes DOI Creative Commons

Shuang Liu,

Ming‐Yi Chou, Gian Maria Niccolò Benucci

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 13(1)

Опубликована: Ноя. 26, 2024

Switchgrass (Panicum virgatum L.) is considered a sustainable biofuel feedstock, given its fast-impact growth, low input requirements, and high biomass yields. Improvements in bioenergy conversion efficiency of switchgrass could be made by reducing lignin content. Engineered that expresses bacterial 3-dehydroshikimate dehydratase (QsuB) has reduced content improved saccharification due to the rerouting shikimate pathway towards simple aromatic protocatechuate at expense biosynthesis. However, impacts this QsuB trait on microbiome structure function remain unclear. To address this, wild-type QsuB-engineered were grown field soils, samples collected from inflorescences, leaves, roots, rhizospheres, bulk soils for analysis. We investigated how expression influenced switchgrass-associated fungal communities using high-throughput Illumina MiSeq amplicon sequencing ITS 16S rDNA. Compared wild-type, hosted different microbial rhizosphere, leaves. Specifically, plants had lower relative abundance arbuscular mycorrhizal fungi (AMF). Additionally, fewer Actinobacteriota root rhizosphere samples. These findings may indicate changes plant metabolism impact both AMF similarly or potential interactions between community. This study enhances understanding plant-microbiome providing baseline data developing beneficial bioengineering strategies assessing nontarget engineered traits microbiome. Bioenergy crops provide an important strategy mitigating climate change. Reducing improve fermentable sugar yields more efficient into bioproducts. In study, we assessed impacted aboveground belowground Our results show unexpected reductions mycorrhizas actinobacteria tissues, raising questions resilience genetically agricultural systems.

Язык: Английский

Процитировано

0
Interactions between grasses and soil biota : From genes to climate change-induced drought DOI Open Access
Paola Rallo

Опубликована: Сен. 25, 2023

Plants can benefit from interacting with their soil microbiomes.To harness these interactions for improved plant performance, it is necessary to understand the genetic basis of effects on microbiome composition.To date, only few studies have aimed pinpoint variants that affect composition, and grasses this issue remains largely unexplored.Using 16S ITS amplicon sequencing, here we characterized in rhizospheres root endospheres 154 genotypes Lolium perenne, an important turf forage grass species.Using available single nucleotide polymorphisms (SNPs) data, used a genome-wide association study (GWAS) identify candidate genes associated variation microbiomes.Our results suggest bacterial fungal communities are modulated by similar biological processes, which pathways related defenses development strongly implicated.Genes lateral formation seem particularly relevant shaping plant-fungal interactions.In endosphere microbiomes, perenne had stronger influence than plant-bacterial communities.Our adds growing evidence within species modulates provides insight mechanisms plants control composition.Genome-wide pinpoints loci rhizosphere | 25Genome-wide 27Genome-wide 29Genome-wide 35Genome-wide 37Genome-wide 43Genome-wide 45Genome-wide

Язык: Английский

Процитировано

0
Bacterial assembly in the switchgrass rhizosphere is shaped by phylogeny, host genotype, and growing site DOI Creative Commons
Jeremy Sutherland, Terrence H. Bell, Stacy A. Bonos

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 17, 2023

Summary Since microbial traits are conserved at different taxonomic levels, plant hosts may influence microbiome composition differently levels to broadly promote or resist microbiota with that impact host fitness. We tested this hypothesis by assessing signals of genetic on bacterial in the switchgrass rhizosphere using 128 genotypes dissimilar growing sites. employed three common gardens, combined mapping, 16S rRNA gene sequence analysis, hierarchical modeling, tests phylogenetic conservation influence, and genome-wide association analyses determine contributions genetics shaping levels. Modeling assembly showed site was a strong factor rhizosphere, though played significant role. The heritability abundance strongest genus level. Phylogenetic signal for detected within phylogeny but clades differed between gardens. identified shared variants associated related metabolism. Our results suggest further investigation is required regarding genotype-by-environment-by-microbiome relationship elucidate factors agroecological dynamics phenotype.

Язык: Английский

Процитировано

0
The core microbiota across the green lineage DOI Creative Commons
Paloma Durán

Current Opinion in Plant Biology, Год журнала: 2023, Номер 77, С. 102487 - 102487

Опубликована: Дек. 5, 2023

The study of plant–microbe interactions and the characterization plant-associated microbiota has been focus plant researchers in last decades due to its importance for health natural conditions. Here, I explore persistent core associated with different species across environments by performing a meta-analysis publicly available datasets. Intra-specific analyses revealed that diverse genotypes growing similar habitats interact common set microbial groups but some these are species- or environment-specific. Furthermore, interspecific demonstrates conservation seven bacterial orders photosynthetic organisms, including microalgae, suggesting conserved capacity interaction microbes throughout evolutionary history. However, specific functions members whether hosts remain largely unexplored. therefore discuss understanding roles propose future research directions, exploration kingdoms. By investigating functions, it will be possible leverage this knowledge sustainable agricultural management goals.

Язык: Английский

Процитировано

0