Targeted gene knockout via CRISPR/Cas9: precise genome editing in eggplant (Solanum melongena) through phytoene desaturase gene disruption

Journal of Crop Science and Biotechnology, Год журнала: 2023, Номер 27(2), С. 249 - 259

Опубликована: Ноя. 9, 2023

Язык: Английский

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A prime example of precisely delivered DNA

Trends in Genetics, Год журнала: 2023, Номер 39(10), С. 717 - 718

Опубликована: Июль 20, 2023

Язык: Английский

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Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 13, 2024

Abstract In the ongoing plant-pathogen arms race, plants employ pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs), while in successful pathogens, PAMPs can evolve evade detection. Engineering PRRs evading could potentially generate broad-spectrum and durable disease resistance. this study, we reverse-engineered two natural FLAGELLIN SENSING 2 (FLS2) variants, VrFLS2XL GmFLS2b, with extended specificities towards flg22 variants. We identified minimal gain-of-function residues enabling blind FLS2s otherwise uncovered strategies: (i) enhancing FLS2-flg22 interaction around flg22’s key evasion sites, (ii) strengthening direct between FLS2 its co-receptor BAK1 overcome weak agonistic antagonistic flg22s, respectively. Additionally, leveraged polymorphisms that enhance through unknown mechanisms engineer superior capability. These findings offer basic design principles for broader spectra, paving way PRR engineering using precise gene-editing increase resistance crops.

Язык: Английский

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Identification of a key gain-of-function residue for effector binding byin vitroshuffling of barleyMla NLRgenes

Опубликована: Окт. 29, 2024

Abstract Natural plant populations maintain high resistance ( R ) gene diversities that provide effective pathogen resistance; however, such diversity has been reduced significantly by the genetic bottlenecks associated with domestication and breeding. Agricultural crops typically contain limited so is often short-lived as pathogens evolve rapidly to evade recognition. The mildew locus A Mla family of barley wheat represents a rich source natural variation ideal for mining disease specificities. genes encode immune receptor proteins nucleotide-binding leucine-rich repeat (NLR) class recognise unrelated binding secreted virulence termed effectors. NLRs MLA13 MLA7 confer different strains powdery through direct interaction effectors AVR A13 A7 respectively. Using DNA shuffling, we generated variant library recombining Mla7 Mla13 in vitro . was cloned into yeast generating ∼4,000 independent clones screened using yeast-two-hybrid (Y2H) assay. This yielded number NLR interacted Sequence analysis showed interacting MLA can be clustered three groups, all which critical residue from MLA13. While differ 30 residues across LRR domain, replacement leucine serine at this position facilitated -dependent signalling planta We have established pipeline evolves MLAs distinct without requirement protein structural knowledge use rationale design. suggest these findings represent step towards evolving novel recognition capabilities

Язык: Английский

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No plant is an island

Current Biology, Год журнала: 2023, Номер 33(11), С. R453 - R455

Опубликована: Июнь 1, 2023

Язык: Английский

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