bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 3, 2024
ABSTRACT
In
prokaryotes
lateral
gene
transfer
(LGT)
is
a
key
mechanism
leading
to
intra-species
variability
in
content
and
the
phenomenon
of
pangenomes.
microbial
eukaryotes,
however,
extent
which
LGT-driven
pangenomes
exist
unclear.
Pelagophytes
are
ecologically
important
marine
algae
that
include
Aureococcus
anophagefferens
–
species
notorious
for
causing
harmful
algal
blooms.
To
investigate
genome
evolution
across
Pelagophyceae
within
,
we
used
long-read
sequencing
produce
high-quality
assemblies
five
strains
Ac.
(52-54
megabase-pairs;
Mbp),
telomere-to-telomere
assembly
Pelagomonas
calceolata
(32
first
reference
Aureoumbra
lagunensis
(41
Mbp).
Using
comparative
genomics
phylogenetics,
show
remarkable
strain
level
genetic
variation
with
pangenome
(23,356
orthogroups)
81.1%
core
18.9%
accessory.
Although
does
not
appear
be
largely
driven
by
recent
prokaryotic
LGTs
(2.6%
accessory
orthogroups),
368
orthogroups
were
acquired
from
bacteria
common
ancestor
all
analyzed
found
P.
or
Au.
.
1,077
viruses
identified
overall,
constituting
3.5-4.0%
each
species.
This
includes
genes
likely
contributing
ecological
success
pelagophytes
globally
long-lasting
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 30, 2024
The
catastrophic
loss
of
aquatic
life
in
the
Central
European
Oder
River
2022,
caused
by
a
toxic
bloom
haptophyte
microalga
Prymnesium
parvum
(in
wide
sense,
s.l.),
underscores
need
to
improve
our
understanding
genomic
basis
toxin.
Previous
morphological,
phylogenetic,
and
studies
have
revealed
cryptic
diversity
within
P.
s.l.
uncovered
three
clade-specific
(types
A,
B,
C)
prymnesin
toxins.
Here,
we
used
state-of-the-art
long-read
sequencing
assembled
first
haplotype-resolved
diploid
genome
type
strain
responsible
for
disaster.
Comparative
analyses
with
A
genomes
genome-size
expansion
driven
repetitive
elements
B.
We
also
found
conserved
chromosomal
synteny
but
divergent
evolution
several
polyketide
synthase
(PKS)
genes,
which
are
known
underlie
toxin
production
combination
environmental
cues.
identified
specific,
approximately
20
kilobase
pair
comprising
deletion
largest
PKS
gene
B
that
link
differences
chemical
structure
types
prymnesins.
Electron-microscopy
flow
cytometry
confirmed
diploidy
closely
related
strains
morphology
ploidy.
Our
results
provide
unprecedented
resolution
better
variability
haptophytes.
reference-quality
will
help
understand
changes
microbial
face
increasing
pressures,
provides
strain-level
monitoring
invasive
future.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 3, 2024
ABSTRACT
In
prokaryotes
lateral
gene
transfer
(LGT)
is
a
key
mechanism
leading
to
intra-species
variability
in
content
and
the
phenomenon
of
pangenomes.
microbial
eukaryotes,
however,
extent
which
LGT-driven
pangenomes
exist
unclear.
Pelagophytes
are
ecologically
important
marine
algae
that
include
Aureococcus
anophagefferens
–
species
notorious
for
causing
harmful
algal
blooms.
To
investigate
genome
evolution
across
Pelagophyceae
within
,
we
used
long-read
sequencing
produce
high-quality
assemblies
five
strains
Ac.
(52-54
megabase-pairs;
Mbp),
telomere-to-telomere
assembly
Pelagomonas
calceolata
(32
first
reference
Aureoumbra
lagunensis
(41
Mbp).
Using
comparative
genomics
phylogenetics,
show
remarkable
strain
level
genetic
variation
with
pangenome
(23,356
orthogroups)
81.1%
core
18.9%
accessory.
Although
does
not
appear
be
largely
driven
by
recent
prokaryotic
LGTs
(2.6%
accessory
orthogroups),
368
orthogroups
were
acquired
from
bacteria
common
ancestor
all
analyzed
found
P.
or
Au.
.
1,077
viruses
identified
overall,
constituting
3.5-4.0%
each
species.
This
includes
genes
likely
contributing
ecological
success
pelagophytes
globally
long-lasting
