bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Окт. 13, 2023
To gain insights into how juvenile hormone (JH) came to regulate insect metamorphosis, we studied its function in the ametabolous firebrat,
Язык: Английский
National Science Review, Год журнала: 2025, Номер unknown
Опубликована: Янв. 23, 2025
Inter-organelle communication coordinates cellular homeostasis and function. Juvenile hormone (JH) is produced in the corpora allata (CA) acts as a gonadotrophic most insects. Using transcriptomic, biochemical, molecular, genetic analyses, here we investigated underlying mechanism of how inter-organelle dynamically orchestrates JH biosynthesis female reproduction American cockroach, Periplaneta americana. The extracellular stimuli insulin allatostatin act through their membrane receptors antagonistically regulate RyR-mediated Ca2+ release from endoplasmic reticulum CA cells. Ca2+-activated CaMKII stimulates energy metabolism mitochondria partially via SLC25A6, induces expression biosynthetic genes HMGR, Jhamt, Cyp15a1 activating transcription factor CREB, which recruits CBP for histone acetylation nucleus. Additionally, interact with CREB-CBP mitonuclear to biosynthesis. From perspective communication, this comprehensive study significantly advanced our understanding reproductive biology
Язык: Английский
Процитировано
2
Development, Год журнала: 2024, Номер 151(7)
Опубликована: Апрель 1, 2024
ABSTRACT The germline provides the genetic and non-genetic information that passes from one generation to next. Given this important role in species propagation, egg sperm precursors, called primordial germ cells (PGCs), are of first cell types specified during embryogenesis. In fact, PGCs form well before bipotential somatic gonad is specified. This common feature development necessitates migrate through many tissues reach gonad. During their journey, must respond select environmental cues while ignoring others a dynamically developing embryo. complex multi-tissue, combinatorial nature PGC migration an excellent model for understanding how navigate environments vivo. Here, we discuss recent findings on migratory path, shepherd PGCs, guidance provide, response these establish pool future generations. We end by discussing fate wayward fail diverse species. Collectively, field poised yield insights into emerging reproductive technologies.
Язык: Английский
Процитировано
6
BMC Biology, Год журнала: 2024, Номер 22(1)
Опубликована: Окт. 11, 2024
Juvenile hormone (JH) is an insect-exclusive involved in regulating diverse aspects of insect physiology, and the evolution its function widely interesting. Studying embryogenesis basal wingless insects important to understand functional JH; however, experimental studies this regard are scarce. In study, we conducted CRISPR/Cas9-mediated knockout (KO) genes JH biosynthesis signaling cascades ametabolous firebrat, Thermobia domestica. Additionally, investigated whether primitive action conserved hemimetabolous cricket, Gryllus bimaculatus. We observed that KO JHAMT, CYP15A1, Met, Kr-h1 resulted embryonic lethality T. Deprivation or arrested progression extraembryonic fluid resorption after dorsal closure hatching, which consistent with gene expression pattern showing high late embryos The deficient displayed wrinkled weak legs. Comparative transcriptome analysis revealed promotes leg maturation through inducing energy supply muscle activity, as validated by transmission electron microscopy (TEM). addition, exhibited similar effects G. This study reveals indispensable role facilitating terminal tissues during embryogenesis, demonstrated regulation development, insects. These findings further indicate functions evolved earlier than anti-metamorphic postembryonic development.
Язык: Английский
Процитировано
4
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(46)
Опубликована: Ноя. 4, 2024
Caste differentiation involves many functional traits that diverge during larval growth and metamorphosis to produce adults irreversibly adapted reproductive division of labor. Investigating developmental is important for general biological understanding has increasingly been explored social phenotypes in parallel from similar genotypes. Here, we use Monomorium pharaonis ants investigate the extent which canalized worker development can be shifted toward gyne (virgin-queen) by juvenile hormone (JH) treatment. We show excess JH activate gyne-biased workers so wing-buds, ocelli, antennal genital imaginal discs, flight muscles, gyne-like fat bodies brains emerge after pupation. However, ovary remained unresponsive treatment, indicating JH-sensitive germline sequestration happens well before somatic differentiation. Our findings reveal qualitative restrictions treatment redirect these constraints are independent body size. corroborate a key inducing caste but this process asymmetric higher colony-level versus superorganisms as defined century ago Wheeler. quantified gene expression changes response throughout identified set genes responsible emergence traits. study suggests gonadotropic role maturation individual level solitary insects colony an evolutionary-derived highly polygynous superorganism like pharaoh ant.
Язык: Английский
Процитировано
4
Genetics, Год журнала: 2025, Номер unknown
Опубликована: Апрель 4, 2025
Abstract The continuity of a species depends on germ cells. Germ cells are different from all the other cell types body (somatic cells) as they solely destined to develop into gametes (sperm or egg) create next generation. In this review, we will touch 4 areas embryonic development in Drosophila melanogaster: assembly and function germplasm, which houses determinants for specification fate mitochondria generation; process pole formation, give rise primordial (PGCs); toward PGC fate; finally, migration PGCs somatic gonadal precursors, where they, together with form testis ovary.
Язык: Английский
Процитировано
0
Journal of Asia-Pacific Entomology, Год журнала: 2025, Номер unknown, С. 102410 - 102410
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Journal of Lipid Research, Год журнала: 2025, Номер unknown, С. 100815 - 100815
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
eLife, Год журнала: 2023, Номер 12
Опубликована: Ноя. 21, 2023
To gain insights into how juvenile hormone (JH) came to regulate insect metamorphosis, we studied its function in the ametabolous firebrat, Thermobia domestica. Highest levels of JH occur during late embryogenesis, with only low thereafter. Loss-of-function and gain-of-function experiments show that acts on embryonic tissues suppress morphogenesis cell determination promote their terminal differentiation. Similar actions hemimetabolous insects short germ band embryos indicate JH's role preceded derived as postembryonic regulator metamorphosis. The expansion likely followed evolution flight. Archaic flying were considered lack metamorphosis because tiny, movable wings evident thoraces young juveniles positive allometric growth eventually allowed them support flight juveniles. Like Thermobia, assume these lacked JH. However, a reappearance wing redirected development make pad rather than wing. Maintenance then disappearance mature Subsequent modification action for hemi- holometabolous lifestyles are discussed.
Язык: Английский
Процитировано
8
BMC Biology, Год журнала: 2024, Номер 22(1)
Опубликована: Май 13, 2024
Abstract Background Juvenile hormones (JH) play crucial role in regulating development and reproduction insects. The most common form of JH is III, derived from MF through epoxidation by CYP15 enzymes. However, the higher dipterans, such as fruitfly, Drosophila melanogaster , a bis-epoxide JHB3, accounted detected. Moreover, these dipterans have lost gene their genomes. As result, identity P450 epoxidase biosynthesis pathway remains unknown. Results In this study, we show that Cyp6g2 serves major responsible for JHB3 III D. . predominantly expressed corpus allatum (CA), concurring with expression pattern jhamt another well-studied last steps biosynthesis. Mutation leads to severe disruptions larval-pupal metamorphosis exhibits reproductive deficiencies, exceeding those seen mutants. Notably, −/− ::jhamt 2 double mutants all died at pupal stage but could be rescued topical application analogs. titer analyses revealed both mutant lacking while overexpression or caused significant increase titer. Conclusions These findings collectively established laid groundwork further understanding pave way developing specific inhibitors insect growth regulators insecticides.
Язык: Английский
Процитировано
3
Current Opinion in Insect Science, Год журнала: 2024, Номер 67, С. 101289 - 101289
Опубликована: Окт. 30, 2024
Язык: Английский
Процитировано
3